Speciation of essential and toxic elements in edible mushrooms: size‐exclusion chromatography separation with on‐line UV–inductively coupled plasma mass spectrometry detection

Abstract

Size-exclusion liquid chromatography was coupled to UV and inductively coupled plasma mass spectrometry (ICP-MS) for detection to perform elemental speciation studies on different edible mushrooms. Molecular weight (MW) distribution patterns of several elements among different fractions present in various edible mushrooms are presented. The association of the elements with the high and low MW fractions was observed using sequential detection by UV and ICP-MS. Separation was performed using a Superdex 75 column. Variability of the fractionation patterns with three different extraction media (0.05 mol l −1 NaOH; 0.05 mol l −1 HCl; hot water at 60 • C) was evaluated for mushroom species. A comparative elemental speciation study was performed in order to determine the differences in the fractionation patterns of silver, arsenic, cadmium, mercury, lead, and tin in Boletus edulis, Agaricus bisporus, and Lentinus edodes. Differences in the fractionation patterns of the elements were found to depend on the mushroom species and the extraction medium. Most of the elements were associated with high mw fractions. It was not possible to assess the trace metal contributions from the mushroom growth media.

Atomic spectrometry update. Environmental analysis

“…SEC was used 122 along with ion chromatography, both coupled with on-line ICP-MS detection, to investigate thallium speciation in the Tl-hyperaccumulating plant Iberis intermedia: Tl I was found to be the dominant chemical form in roots, stems and leaves. Wuilloud et al 123,124 used SEC-ICP-MS to study trace element speciation in edible mushrooms. Separation into high and low molecular weight fractions was achieved with a Superdex 75 column.…”

“…Kumar et al 226 developed a rapid method for synthesizing a mixed standard of phenyltin species enriched with 124 Sn based on the phenylation of tin iodide and 124 Sn enriched metal. Using GC-ICP-MS with SS-ID calibration, the isotopeenriched standard was employed to evaluate different extraction procedures currently in use for the determination of phenyltin species in sediments.…”

Nitrogen doped nano porous graphene as a sorbent for separation and preconcentration trace amounts of Pb, Cd and Cr by Ultrasonic assisted in‐syringe dispersive micro solid phase extraction

“…Several analytical techniques such as flame atomic absorption spectrometry (FAAS), inductively coupled plasma atomic emission spectrometry (ICP‐AES), electro‐thermal atomic absorption spectrometry (ETAAS), inductively coupled plasma‐optical emission spectrometry (ICP‐OES), and inductively coupled plasma mass spectrometry (ICP‐MS) have been used for the measuring of metal ions. Among these methods, FAAS usually is used as low cost, easy operation, and high sample throughput method.…”

Non-chromatographic speciation of mercury in mushrooms

“…In early studies, Stegnar (1973), Stijve (1974), Minagawa (1980), Bargagli (1984) and Fischer (1995) employed gas chromatography atomic absorption spectroscopy (GC-AAS) with a limit of detection (LOD) for MeHg of about 0.01 mg g À1 . [17][18][19][20][21] Recently, Wuilloud (2004) determined the differences in the fractionation patterns of Hg through size exclusion chromatography inductively coupled plasma mass spectrometry (SEC-UV-ICP-MS) 22 and Rieder (2011) and Pilz (2011) employed GC atomic uorescence spectroscopy (GC-AFS) with a LOD for MeHg of about 0.05 ng g À1 , and liquid chromatography (LC) combined with chemical vapour generation (CVG) and ICP-MS, respectively. 23,24 In the aforementioned studies, MeHg expressed as percentage from t-Hg ranged between 0.01 and 26%.…”