Speciation of iron in breast milk and infant formulas whey by size exclusion chromatography-high performance liquid chromatography and electrothermal atomic absorption spectrometry

Bermejo, Pilar; Peña, E.; Domı́nguez, Raquel; Bermejo, A.; Fraga, José M.; Cocho, José A.

doi:10.1016/s0039-9140(99)00233-7

Cited by 46 publications

(17 citation statements)

References 8 publications

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“…The percentage of iron observed in each region (biomolecules) is given in Table 3. A high percentage of iron (75.9-93.0%) in association to immunoglobulins (>160-180 kDa) and smaller percentages in association to lactoferrin, transferrin or albumin (2-8.5%) in the regions of 70-90 kDa, and in association with low molecular weight molecules (20-10 kDa: 0-4% and b10 kDa: 0.6-17%) are consistent with previous studies [15,[31][32][33][34][35][36]. Some authors have detected iron mainly bound to high molecular weight (540 kDa) biocompounds [31] or lactoferrin [32].…”

Section: Discussionsupporting

confidence: 91%

“…We found that amount of iron eluted in the region of lactoferrin is higher in colostrum than in mature milk (see Table 3), this may be because human colostrum has a very high levels of lactoferrin (tipically up to 5 g/L, which falls to 1.5-2 g/L after 10 days. Conversely, they found that iron profile obtained in commercial formulas was mostly spread over fractions of low molecular weight [32,35]. In our study we found that less than 5% of iron content of commercial formulas was bound to high molecular fractions, while more than 75% was in fractions of 20-10 kDa and b10 kDa, a highly significant difference in Fe speciation in comparison with that found for human milk samples.…”

Section: Discussioncontrasting

confidence: 44%

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Iron content and its speciation in human milk from mothers of preterm and full-term infants at early stages of lactation: A comparison with commercial infant milk formulas

Sánchez

Remy

Iglesias

et al. 2012

Microchemical Journal

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Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 44%

Iron content and its speciation in human milk from mothers of preterm and full-term infants at early stages of lactation: A comparison with commercial infant milk formulas

Sánchez

Remy

Iglesias

et al. 2012

Microchemical Journal

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“…According to statistical reports from 2007, few European women breast-feed their infants exclusively at 6 months of age, and only 33% of infants in the USA are exclusively breast-fed up to 3 months of age (http://ec.europa.eu/ health/ph_projects/2002/promotion/fp_promotion_2002_frep_18_ en.pdf). Thus, infant formula, liquids, or reconstituted powdered food have become useful and convenient substitutes for human milk having a major role in infants' diets due to the source of nutrients (Bermejo et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Probabilistic assessment of the intake of mineral and trace elements by consumption of infant formulas and processed cereal-based food in Spain

Moreno-Rojas

Cañal-Ruiz

Amaro-López

et al. 2014

CyTA - Journal of Food

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In this study a probabilistic model was developed to estimate the intake level of Fe, Zn, Ca, P, Mg, Mn, Cu, Na, and K derived from consumption of infant formulas and processed cereal-based food in Spain. Infant formulas were classified in two categories (first term and follow-on) while cereal-based foods were split up in six groups according to their composition (without gluten, only with gluten, fruits, honey, cocoa, and integral cereal). Contributions to Dietary Reference Intakes (DRIs) were studied for two population groups: 0-6-monthold and 6-month-1-year-old infants. The results reported that mineral and trace elements contents in these types of food cover the established DRIs for Ca, Mg, P, Fe, and K being relatively low for Zn and Cu. Conversely, Na and Mn contents were moderately high and should be decreased to reduce the prevalence of certain diseases.Se ha desarrollado un modelo probabilístico para determinar la ingesta de Fe, Zn, Ca, P, Mg, Mn, Cu, Na, y K a partir del consumo de fórmulas y cereales infantiles. Las fórmulas infantiles se clasificaron en dos categorías (inicio y continuación) y los cereales en seis categorías (con o sin gluten, con frutas, miel, cacao e integrales). Las contribuciones a la Ingestas Dietéticas de Referencia (IDR) se han realizado para dos grupos de población: bebés de 0-6 meses y de 6 meses-1 año. Los resultados muestran como el contenido de minerales y elementos traza presentes en este tipo de alimentos cubren la IDR establecida para Ca, Mg, P, Fe, K, y fueron bajos para cubrir la IDR de Zn y Cu. Por contra, el contenido de Na y Mn es alto y debe reducirse para reducir la incidencia de determinadas patologías.

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“…12,[16][17][18][19][20][21] In addition, several works in the literature have described elemental speciation in milk samples by size exclusion chromatography (SEC), 22 in combination with specific element detectors, such as electrothermal atomic absorption spectrometry (ET AAS), 18,23 inductively coupled plasma optical emission spectrometry (ICP OES) 24,25 and inductively coupled plasma mass spectrometry (ICP-MS). 4,16,17 Of these techniques, the most appropriate are ET AAS and ICP-MS, due to their low detection limits for trace elements.…”

Section: Introductionmentioning

confidence: 99%

Studying the distribution of Selenium in buffalo and cow's milk whey

Pereira¹,

Fernandes²

2011

J. Braz. Chem. Soc.

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Neste estudo, a cromatografia por exclusão de tamanho (SEC) com detecção online ultravioleta (UV), espectrometria de absorção atômica em forno de grafite (GF AAS) e a espectrometria de massa por tempo de voo com dessorção/ionização de matriz assistida por laser (MALDI-TOF-MS) foram usadas para estudar a associação de selênio com macromoléculas e compostos presentes no soro de leite de búfala e bovino. Os perfis de SEC-UV obtidos para as amostras de soro de leite de búfala e soro de leite bovino indicaram a presença de espécies de alta e baixa massa molecular. A combinação das informações obtidas com SEC-UV, GF AAS e MALDI-TOF-MS para as frações < 10 kDa confirmou a associação de selênio com espécies de baixa massa molecular.In this study, size exclusion chromatography (SEC) coupled with an online ultraviolet detection (UV) and graphite furnace atomic absorption spectrometry (GF AAS) and matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF-MS) were used to study the association of selenium with macromolecules and compounds present in buffalo and cow's milk whey. SEC-UV profiles obtained from buffalo and cow's milk whey samples indicate the presence of high molecular weight (HMW) and low molecular weight (LMW) species in both samples studied. The combined information obtained with SEC-UV, GF AAS and MALDI-TOF-MS for fractions < 10 kDa confirmed the association of selenium with LMW species. Keywords: buffalo and cow's milk whey, SEC offline GF AAS, SEC online UV, MALDI-TOF-MS IntroductionBuffalo (Bubalus bubalis) milk is a food highly nutritious and beneficial to the human health due to the biological value of its constituents. It has pronounced differences when compared to cow's milk, due to the presence of greater percentages of constituents such as fat, protein, lactose, total solids and some minerals. 1 Buffalo milk is used in northern Brazil for making different dairy products, including butter, various cheeses and yogurt. 2 However, studies concerning the chemical composition of buffalo milk are limited compared with cow's milk ones.Milk proteins are classified according to their structural and physicochemical properties, and consist of the following: casein, whey proteins, fat globule membrane proteins, enzymes and growth factors. However, from a nutritional and industrial perspective, casein and whey proteins are more widely applicable and have significant economic value. The total protein concentration and ratio of casein and whey protein fractions vary considerably in milk from different species. a-lactalbumin, bovine serum albumin, immunoglobulin, lactoferrin and lysozyme are whey proteins that provide health benefits. b-lactoglobulin, which is not found in human milk, 3 is the most abundant protein in cow's milk whey and is associated with allergies, particularly in children.The elemental composition of food plays important biological roles. However, the bioavailability and toxicity of these elements strongly depend on the chemical form in which they occur in biologica...

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Speciation of iron in breast milk and infant formulas whey by size exclusion chromatography-high performance liquid chromatography and electrothermal atomic absorption spectrometry

Cited by 46 publications

References 8 publications

Iron content and its speciation in human milk from mothers of preterm and full-term infants at early stages of lactation: A comparison with commercial infant milk formulas

Iron content and its speciation in human milk from mothers of preterm and full-term infants at early stages of lactation: A comparison with commercial infant milk formulas

Probabilistic assessment of the intake of mineral and trace elements by consumption of infant formulas and processed cereal-based food in Spain

Studying the distribution of Selenium in buffalo and cow's milk whey

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