Speciation of Selenium in Biological Samples by Ion Chromatography With Inductively Coupled Plasma Mass Spectrometry

Abstract

& A comprehensive review is presented addressing recent trends in the speciation and determination of selenium in biological sample matrices, including important analytical aspects such as sample clean up, pre-concentration, method development, and removal of instrumental interferences. A brief outline of analytical principles, together with an overview of the recent developments and applications of selenium speciation determination is included. The newer methods for detecting selenium, including preparation schemes are also discussed together with speciation methodology based on ion chromatography hyphenated with inductively coupled plasma mass spectrometric techniques.

Overview of hyphenated techniques using an ICP-MS detector with an emphasis on extraction techniques for measurement of metalloids by HPLC–ICPMS

“…As and Se [42] can cause signal enhancement. The majority of published speciation measurements have used anion, cation, C8 or C18 reverse phase columns [43][44][45][46] utilizing aqueous phases or mobile phases with only 5-10% v/v of an organic solvent to reduce the retention times of species. However, with the interest in organometallic species such as arsenic lipids [47,48], the use of desolvating systems [49] and oxygen as an auxiliary gas [50] allows the use of high concentrations of organic solvents in mobile phases.…”

Atomic spectrometry update. Elemental speciation

“…The importance of Se biochemistry has produced two reviews of the determination of relevant compounds by chromatography with ESD. 47,48 Finally, the chemical methodology that underpins the on-going monitoring of the environment for OTCs, particularly TBT and TPhT, has been reviewed 49 with particular attention to extraction, derivatization and cleanup 2 Sample preparation 2…”

“…The importance of Se biochemistry has produced two reviews of the determination of relevant compounds by chromatography with ESD. 47,48 Finally, the chemical methodology that underpins the on-going monitoring of the environment for OTCs, particularly TBT and TPhT, has been reviewed 49 with particular attention to extraction, derivatization and cleanup 2 Sample preparation 2.1 Analyte stability It has been pointed out 50 that the literature concerned with the preservation of arsenic species in environmental water samples is confusing and contradictory by reviewers who consider that microbially mediated reactions and those with the Fe II /Fe III redox system are not properly controlled. The reviewers conclude that filtration (as soon as possible), refrigeration and storage in the dark are prerequisites for the stabilization of As III and As V , as these processes remove suspended matter and microbes, suppress biotic and abiotic reactions, and prevent photochemical reactions, respectively.…”

Atomic spectrometry update. Clinical and biological materials, foods and beverages

“…15 The reviewers highlighted the complications associated with the quantitative determination and identification of Se species and noted that improvements in the standardization and availability of techniques for species quantification were required. Another group 153 presented a comprehensive review on recent trends in the determination and speciation of Se in biological matrices using chromatographic and ICP-mass spectrometric techniques. The review addressed important aspects such as sample preparation and interferences on quantitative determination.…”