Speciation of Serum Copper and Zinc-Binding High- and Low-Molecular Mass Ligands in Dairy Cows Using HPLC-ICP-MS Technique

Atomic spectrometry update: review of advances in elemental speciation

“…Using the Hepes buffer, a value of 10 10 mol À1 was found for the affinity of the small b-amyloid 1-16 for Cu 2+ , which is in agreement with the values obtained for other proteins involved in neurodegenerative diseases. A method for the assessment of the major Cu and Zn containing molecular species in dairy cow blood serum used SEC for their separation with detection using ICP-MS. 178 Speciation of serum Cu and Zn was performed using a Bio SEC-5 column (7.8 Â 300 mm, pore size 300 Å) packed with spherical, high purity porous silica with a hydrophilic polymeric coating. This gave a separation prole in the range of 5 to 1250 kDa.…”

Atomic Spectrometry Update: review of advances in elemental speciation

“…Finally, SEC-ICP-MS was applied to determine HMM and LMM Cu and Zn compounds in dairy cow blood serum. 178 A total of 17 5-6 year old female cows were examined. Blood samples were collected, centrifuged and the separated serum was stored at À70 C. Prior to analysis, samples were diluted with a solution containing 1% butanol-1, 1% TRITON X-100 and 0.07% HNO 3 .…”

“…Finally, SEC-ICP-MS was applied to determine HMM and LMM Cu and Zn compounds in dairy cow blood serum. 178 A total of 17 5–6 year old female cows were examined. Blood samples were collected, centrifuged and the separated serum was stored at −70 °C.…”

“…Using the Hepes buffer, a value of 10 10 mol −1 was found for the affinity of the small β-amyloid 1–16 for Cu 2+ , which is in agreement with the values obtained for other proteins involved in neurodegenerative diseases. A method for the assessment of the major Cu and Zn containing molecular species in dairy cow blood serum used SEC for their separation with detection using ICP-MS. 178 Speciation of serum Cu and Zn was performed using a Bio SEC-5 column (7.8 × 300 mm, pore size 300 Å) packed with spherical, high purity porous silica with a hydrophilic polymeric coating. This gave a separation profile in the range of 5 to 1250 kDa.…”

A novel approach for the determination of exchangeable copper in serum using protein precipitation

“…Despite being very accurate, analysis times per sample are very long and the methods require elaborate instrumentation. [35][36][37][38] CuEXC can also be assessed via Cu 2+ -specic uorescence sensors. The uorophores form a strong complex with Cu 2+ , which quenches the uorescence of the sensor according to the concentration of Cu 2+ in serum.…”