Species boundaries in the Ophrys iricolor group in Tunisia: do local endemics always matter?

Gögler, Julia; Zitari, Anis; Paulus, Hannes F.; Cozzolino, Salvatore; Ayasse, Manfred

doi:10.1007/s00606-015-1271-5

Cited by 7 publications

(2 citation statements)

References 25 publications

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“…Although (Z)-11-C23/C25enes are found in other Orchidinae taxa with different pollination mechanisms, the production of these compounds does not show any clear association with any other pollinator guild (i.e., ants, bees, flies excluding tachinids, moths, and wasps). The compounds (Z)-11-C23/C25enes have also been recorded among sexually deceptive (i.e., Ophrys; Mant et al, 2005;Stökl et al, 2005Stökl et al, , 2007Stökl et al, , 2008Stökl et al, , 2009Gögler et al, 2016;Robustelli della Cuna et al, 2019) and non-sexually deceptive orchids with mixed systems (i.e., Anacamptis, Cleisostoma, Neotinea, Serapias; Schiestl & Cozzolino, 2008;Pellegrino et al, 2012;Robustelli della Cuna et al, 2019;Son et al, 2020;Martel et al, 2021). Although Ophrys species have also been recorded producing high relative concentrations of (Z)-11-C23/C25enes, this is represented exclusively by (Z)-11pentacosene since no production of (Z)-11-tricosene was recorded (Mant et al, 2005;Stökl et al, 2005Stökl et al, , 2007Stökl et al, , 2008Stökl et al, , 2009Gögler et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The compounds (Z)-11-C23/C25enes have also been recorded among sexually deceptive (i.e., Ophrys; Mant et al, 2005;Stökl et al, 2005Stökl et al, , 2007Stökl et al, , 2008Stökl et al, , 2009Gögler et al, 2016;Robustelli della Cuna et al, 2019) and non-sexually deceptive orchids with mixed systems (i.e., Anacamptis, Cleisostoma, Neotinea, Serapias; Schiestl & Cozzolino, 2008;Pellegrino et al, 2012;Robustelli della Cuna et al, 2019;Son et al, 2020;Martel et al, 2021). Although Ophrys species have also been recorded producing high relative concentrations of (Z)-11-C23/C25enes, this is represented exclusively by (Z)-11pentacosene since no production of (Z)-11-tricosene was recorded (Mant et al, 2005;Stökl et al, 2005Stökl et al, , 2007Stökl et al, , 2008Stökl et al, , 2009Gögler et al, 2016). To the best of our knowledge, (Z)-11tricosene in Ophrys flowers is only known for O. holoserica (Burm.…”

Section: Discussionmentioning

confidence: 99%

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The evolution of tachinid pollination in Neotinea ustulata is related to floral cuticular composition and the combined high relative production of (Z)‐11‐C23/C25enes

Martel

Rákosy

Romero

et al. 2021

J of Sytematics Evolution

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Among terrestrial orchids, and particularly among the subtribe Orchidinae, flies are underrepresented as pollinators. The European Neotinea ustulata, which developed specialized pollination by tachinid flies, is known to produce high relative concentrations of the floral cuticular alkenes (Z)-11-tricosene and (Z)-11-pentacosene (referred to as (Z)-11-C23/C25enes), which seem to be uncommon among orchid flowers. If the evolution of tachinid pollination is related to that of (Z)-11-C23/C25enes, we can expect that closely related species have a different floral chemical pattern and significantly small or no production of (Z)-11-C23/C25enes, independently of their pollinator guild identity (e.g., bees, flies, moths). We chemically compared the floral cuticular composition among Neotinea species, performed electrophysiological analyses, reconstructed the phylogenetic Orchidinae tree, and identified the evolutionary history of pollinator guild and (Z)-11-C23/C25enes production within the Orchidinae. Neotinea ustulata has evolved a markedly different floral cuticular composition compared to other Neotinea and produces both compounds ((Z)-11-C23/C25enes) in high relative quantities (i.e., above 8% in combination), which are detectable by tachinid antennae. Moreover, most Orchidinae taxa have minimal or no production of these alkenes, independently of the identity of their pollinator guild. Our ancestral reconstruction suggested that (Z)-11-C23/C25enes production was an evolutionary exaptation in Neotinea, whereas tachinid pollination was a unique evolutionary innovation for N. ustulata. Floral cuticular composition and, in particular, the combined production of (Z)-11-C23/C25enes at relatively high concentrations is intimately linked to the evolution of tachinid pollination within the Orchidinae.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The evolution of tachinid pollination in Neotinea ustulata is related to floral cuticular composition and the combined high relative production of (Z)‐11‐C23/C25enes

Martel

Rákosy

Romero

et al. 2021

J of Sytematics Evolution

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Sexual Deception in the Eucera-Pollinated Ophrys leochroma: A Chemical Intermediate between Wasp- and Andrena-Pollinated Species

et al. 2017

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Ophrys flowers mimic sex pheromones of attractive females of their pollinators and attract males, which attempt to copulate with the flower and thereby pollinate it. Virgin females and orchid flowers are known to use the same chemical compounds in order to attract males. The composition of the sex pheromone and its floral analogue, however, vary between pollinator genera. Wasp-pollinated Ophrys species attract their pollinators by using polar hydroxy acids, whereas Andrena-pollinated species use a mixture of non-polar hydrocarbons. The phylogeny of Ophrys shows that its evolution was marked by episodes of rapid diversification coinciding with shifts to different pollinator groups: from wasps to Eucera and consequently to Andrena and other bees. To gain further insights, we studied pollinator attraction in O. leochroma in the context of intra- and inter-generic pollinator shifts, radiation, and diversification in the genus Ophrys. Our model species, O. leochroma, is pollinated by Eucera kullenbergi males and lies in the phylogeny between the wasp and Andrena-pollinated species; therefore, it is a remarkable point to understand pollinator shifts. We collected surface extracts of attractive E. kullenbergi females and labellum extracts of O. leochroma and analyzed them by using gas chromatography with electroantennographic detection (GC-EAD) and gas chromatography coupled with mass spectrometry (GC-MS). We also performed field bioassays. Our results show that O. leochroma mimics the sex pheromone of its pollinator's female by using aldehydes, alcohols, fatty acids, and non-polar compounds (hydrocarbons). Therefore, in terms of the chemistry of pollinator attraction, Eucera-pollinated Ophrys species might represent an intermediate stage between wasp- and Andrena-pollinated orchid species.

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Floral scent in a sexually deceptive Ophrys orchid: from headspace collections to solvent extractions

Zito

Rosselli

Bruno

et al. 2018

Plant Signaling & Behavior

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Species boundaries in the Ophrys iricolor group in Tunisia: do local endemics always matter?

Cited by 7 publications

References 25 publications

The evolution of tachinid pollination in Neotinea ustulata is related to floral cuticular composition and the combined high relative production of (Z)‐11‐C23/C25enes

The evolution of tachinid pollination in Neotinea ustulata is related to floral cuticular composition and the combined high relative production of (Z)‐11‐C23/C25enes

Sexual Deception in the Eucera-Pollinated Ophrys leochroma: A Chemical Intermediate between Wasp- and Andrena-Pollinated Species

Floral scent in a sexually deceptive Ophrys orchid: from headspace collections to solvent extractions

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