In the reservoirs of the Vinnytsia region, we have identified 12 species of naked amoebas. This species: Saccamoeba stagnicola Page, 1974, Saccamoeba sp., Thecamoeba striata (Penard, 1890) Schaeffer, 1926, Stenamoeba stenopodia (Page, 1969) Smirnov et al., 2007, Mayorella cantabrigiensis Page, 1983, Mayorella sp., Korotnevella diskophora Smirnov, 1999, Vexillifera sp., Acanthamoeba sp. (1), Cochliopodium sp., Vahlkampfia sp. (1), Vahlkampfia sp. (2). Two species of naked amoebae (Vahlkampfia sp. (1), Vahlkampfia sp. (2)) belong to heterolobosea amoeba from the group Discoba Simpson in Hampl et al., 2009. In terms of frequency of occurrence, the most common were the amoeba Vahlkampfia sp. (2), Vahlkampfia sp. (1), the least common are Acanthamoeba sp. (1), M. cantabrigiensis, S. stagnicola, Saccamoeba sp., Vexillifera sp., Mayorella sp., K. diskophora, the middle position on the frequency of occurrence is species of Cochliopodium sp., T. striata, S. stenopodia. The species lists of naked amoebas in the reservoirs of the Vinnytsia region are combined into two complexes. The first complex (swamps) contained the amoeba Vahlkampfia sp. (1), in the second (rivers and floodplain water bodies) – S. stagnicola, Saccamoeba sp., T. striata, S. stenopodia, M. cantabrigiensis, Mayorella sp., K. diskophora, Vexillifera sp., Acanthamoeba sp. (1), Cochliopodium sp., Vahlkampfia sp. (1), Vahlkampfia sp. (2). The formation of species complexes of naked amoebae in the water of Vinnytsia region affected by water temperature, water acidity, concentration of dissolved oxygen in the water and organic substances (permanganate oxidizability). The species found by us belong to 7 morphotypes: eruptive, monopodial, striate, lingulate, mayorellian, dactylopodial, acanthopodial, lens-like.