Species Composition of<i>Bacteroidales</i>Order Bacteria in the Feces of Healthy People of Various Ages

Kulagina, Elena V.; Ефимов, Б. А.; Maximov, Phillipp Y.; Кафарская, Л. И.; Chaplin, Andrei V.; Shkoporov, Andrey N.

doi:10.1271/bbb.110434

Cited by 36 publications

(29 citation statements)

References 6 publications

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“…Of the group of 24 OTUs found to be MIT-specific, several were classified as taxa that are associated with healthy microbiota in humans, such as Odoribacter , Parabacteroides merdae , and Alistipes [42]–[45]. One OTU was identified as Clostridium XlVb , a taxon also identified from altered Schaedler flora (ASF) sequences (Table S4), implying that the corresponding organism is closely related to strain ASF 356 in the widely used defined 8-strain rodent ASF [33].…”

Section: Discussionmentioning

confidence: 99%

Intestinal Microbiota Composition of Interleukin-10 Deficient C57BL/6J Mice and Susceptibility to Helicobacter hepaticus-Induced Colitis

et al. 2013

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The mouse pathobiont Helicobacter hepaticus can induce typhlocolitis in interleukin-10-deficient mice, and H. hepaticus infection of immunodeficient mice is widely used as a model to study the role of pathogens and commensal bacteria in the pathogenesis of inflammatory bowel disease. C57BL/6J Il10−/− mice kept under specific pathogen-free conditions in two different facilities (MHH and MIT), displayed strong differences with respect to their susceptibilities to H. hepaticus-induced intestinal pathology. Mice at MIT developed robust typhlocolitis after infection with H. hepaticus, while mice at MHH developed no significant pathology after infection with the same H. hepaticus strain. We hypothesized that the intestinal microbiota might be responsible for these differences and therefore performed high resolution analysis of the intestinal microbiota composition in uninfected mice from the two facilities by deep sequencing of partial 16S rRNA amplicons. The microbiota composition differed markedly between mice from both facilities. Significant differences were also detected between two groups of MHH mice born in different years. Of the 119 operational taxonomic units (OTUs) that occurred in at least half the cecum or colon samples of at least one mouse group, 24 were only found in MIT mice, and another 13 OTUs could only be found in MHH samples. While most of the MHH-specific OTUs could only be identified to class or family level, the MIT-specific set contained OTUs identified to genus or species level, including the opportunistic pathogen, Bilophila wadsworthia. The susceptibility to H. hepaticus-induced colitis differed considerably between Il10−/− mice originating from the two institutions. This was associated with significant differences in microbiota composition, highlighting the importance of characterizing the intestinal microbiome when studying murine models of IBD.

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Section: Discussionmentioning

confidence: 99%

Intestinal Microbiota Composition of Interleukin-10 Deficient C57BL/6J Mice and Susceptibility to Helicobacter hepaticus-Induced Colitis

et al. 2013

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“…Members of this taxonomic group account for 20–40 % of the total bacterial load in human faeces and reach absolute counts of 10 10 –10 11 c.f.u. g − 1 (Arumugam et al , 2011; Kulagina et al , 2012; Lagier et al , 2012a; Rajilić-Stojanović & de Vos, 2014).…”

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confidence: 99%

“…Most species of Alistipes were isolated from the healthy human GI tract microbiota, where they can be found in relatively high titres exceeding 10 9 c.f.u. (g faeces) − 1 (Kulagina et al , 2012). In addition, cases have been reported of isolation of members of the genus Alistipes from blood, appendicular, abdominal, perirectal and brain abscesses, urine and intra-abdominal fluid (Song et al , 2006).…”

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confidence: 99%

Alistipes inops sp. nov. and Coprobacter secundus sp. nov., isolated from human faeces

Shkoporov

Chaplin

Хохлова

et al. 2015

International Journal of Systematic and Evolutionary Microbiology

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Culture-based study of the faecal microbiome in two adult female subjects revealed the presence of two obligately anaerobic, non-spore-forming, rod-shaped, non-motile, Gramnegative bacterial strains that represent novel species. The first strain, designated 627 T , was a fastidious, slow-growing, indole-positive bacterium with a non-fermentative type of metabolism. The strain was characterized by the production of acetic and succinic acids as metabolic end products, the prevalence of iso-C 15 : 0 fatty acid and the presence of menaquinones MK-10 and MK-11. The DNA G+C content was found to be 56.6 mol%. The second strain, designated 177 T , was capable of fermenting a rich collection of carbohydrate substrates, producing acetic acid as a terminal product. The strain was indole-negative and resistant to bile. The major cellular fatty acids were iso-C 15 : 0 and anteiso-C 15 : 0 (in a 1 : 1 ratio) and the predominant menaquinone was MK-11.The DNA G+C content was 37.8 mol%. A phylogenomic analysis of the draft genomes of strains 627 T and 177 T placed these bacteria in the genera Alistipes (family Rikenellaceae) and Coprobacter (family Porphyromonadaceae), respectively. On the basis of the phenotypic and genotypic properties of strains 627 T and 177 T , we conclude that these strains from human faeces represent two novel bacterial species, for which the names Alistipes inops sp. nov. (type strain 627 T 5DSM 28863 T 5VKM B-2859 T ) and Coprobacter secundus sp. nov. (type strain 177 T 5DSM 28864 T 5VKM B-2857 T ) are proposed.

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“…As a part of an ongoing longitudinal study of intestinal Bacteroidales populations in children (Kulagina et al, 2012) a number of faecal samples from a healthy child were collected at different time points and stored frozen as 10 23 dilutions in Columbia broth/15 % (v/v) glycerol. One of the samples, collected at the age of 1 year, was serially diluted in saline, plated on Columbia agar supplemented with 5 % (v/v) horse blood and cultured anaerobically for 72 h at 37 u C. Grown colonies were assessed macroscopically and microscopically and replated aerobically.…”

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confidence: 99%

Coprobacter fastidiosus gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from infant faeces

Shkoporov

Хохлова

Chaplin

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A novel obligately anaerobic, non-spore-forming, rod-shaped, non-motile Gram-reaction-negative bacterium was isolated from infant faeces. The strain, designated NSB1T, was able to grow on rich media at 30–37 °C, in the presence of up to 2 % (w/v) Oxgall and 2 % (w/v) NaCl. Cells of strain NSB1T produced catalase, but not urease and indole. Aesculin was not hydrolysed. The strain was able to utilize d-glucose, lactose, maltose, mannose and raffinose as electron donors. When grown on d-glucose, the main metabolic end products were propionic and acetic acids, with a minor product being succinic acid. The major cellular fatty acids, iso-C15 : 0 and anteiso-C15 : 0, were present at a 1 : 1 molar ratio. The major menaquinone was MK-11. The DNA G+C content was found to be 38.5 mol%. According to 16S rRNA gene sequence analysis strain NSB1T is a member of the family Porphyromonadaceae , phylum Bacteroidetes . The closest relatives of the strain were Barnesiella viscericola (88.2 % identity) and Barnesiella intestinihominis (87.4 % identity). On the basis of phenotypic and genotypic properties of strain NSB1T we conclude that this strain represent a novel species in a new genus within the family of Porphyromonadaceae for which the name Coprobacter fastidiosus gen. nov., sp. nov. is proposed. The type strain of the species is NSB1T ( = DSM 26242T, = VKM B-2743T).

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Species Composition ofBacteroidalesOrder Bacteria in the Feces of Healthy People of Various Ages

Cited by 36 publications

References 6 publications

Intestinal Microbiota Composition of Interleukin-10 Deficient C57BL/6J Mice and Susceptibility to Helicobacter hepaticus-Induced Colitis

Intestinal Microbiota Composition of Interleukin-10 Deficient C57BL/6J Mice and Susceptibility to Helicobacter hepaticus-Induced Colitis

Alistipes inops sp. nov. and Coprobacter secundus sp. nov., isolated from human faeces

Coprobacter fastidiosus gen. nov., sp. nov., a novel member of the family Porphyromonadaceae isolated from infant faeces

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