Species delimitation polyphasic approach reveals Meyerella similis sp. nov.: a new species of “small green balls” within the Chlorella-clade (Trebouxiophyceae, Chlorophyta)

Morphology, phylogeny and fatty acid profiles of Meyerella similis from freshwater ponds and Meyerella krienitzii sp. nov. from soil (Trebouxiophyceae, Chlorophyta)

“…They had a short helix I (only 12 nt), which is the smallest in genus Meyerella and all Chlorella-clade. Also, in the present study, the "molecular signature" of M. similis strains was con rmed (Krivina et al 2023). Combination A-U in 4 bp of helix I remains unique within the genus Meyerella.…”

“…Our strains also had the molecular signature of the genus Meyerella (AA in 152-153 bp, A in 190 bp, T in 242 bp) (Fig. 3), which distinguishes this genus from others within the Chlorella-clade (Krivina et al 2023). The studied ACSSI strains, unlike other Meyerella strains, had a speci c signature -СC (88-89 bp), G (205 bp).…”

“…Phylogenetic analysis of the 18S-ITS1-5.8S-ITS2 fragment showed that the studied strains ACSSI 428 and ACSSI 429 formed an independent phylogenetic lineage within the genus Meyerella. The genetic distances between strains ACSSI 428 and ACSSI 429 were less than 0.1% and corresponded to intraspeci c level both within the genus Meyerella and for the whole Chlorella-clade (Krivina and Temraleeva 2020; Krivina et al 2023). The genetic difference between the studied soil ACSSI strains and aquatic Meyerella species (3.1-3.4%) was signi cantly higher than between the actual aquatic species (2.4%) and corresponded to the interspeci c level.…”

“…After that, it was considered monotypic for quite a long time, although the results of analysis of individual 18S rRNA gene fragments and internal transcribed spacers ITS1 and ITS2 indicated otherwise (Fučikova et al 2014; Lanzoni et al 2016). For some time past another species M. similis was described (Krivina et al 2023). This species inhabits freshwater bodies and includes both free-living organisms and facultative ciliate endosymbionts.…”

Plants, Cells, Algae, and Cyanobacteria In Vitro and Cryobank Collections at the Institute of Plant Physiology, Russian Academy of Sciences—A Platform for Research and Production Center

“…The algae and cyanobacteria collection currently utilizes mid-term preservation at –70 °C to back up some strains from the active collection. Synechocystis mutant strains, model cyanobacteria C. subtropica IPPAS B-1603, Acaryochloris marina IPPAS B-1601, and type strains of novel cyanobacterial and green algal species [ 203 , 209 , 219 , 227 , 228 , 229 , 230 ] are preserved at −70 °C in a freezer with 10% dimethyl sulfoxide or 10% glycerol as cryoprotectors, as described by Iwamoto et al [ 231 ]. The strains C. subtropica IPPAS B-1603 and Spongiosarcinopsis terrestris IPPAS C-2041 were successfully recovered after several months of such storage, and A. marina IPPAS B-1601 was recovered after five years.…”