Species determination and characterization of developmental stages of ticks by whole-animal matrix-assisted laser desorption/ionization mass spectrometry

Karger, Axel; Kampen, Helge; Bettin, Barbara; Dautel, Hans; Ziller, Mario; Hoffmann, Bernd; Süß, Jochen; Klaus, Christine

doi:10.1016/j.ttbdis.2011.11.002

Cited by 83 publications

(88 citation statements)

References 27 publications

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“…Contrary to a recent report (14), we found that the use of tick legs appears to be an effective means to identify tick vectors if a reference database is available. The remainder of the body can be reserved for other purposes, such as the detection of pathogens, as performed in this study.…”

Section: Discussioncontrasting

confidence: 99%

“…A similar discrepancy was observed in a preliminary study using the entire body of the tick (14). Therefore, although MALDI-TOF MS has opened new doors for the phylogenetic study of arthropods and bacteria, additional data are still needed, and detailed studies should be conducted.…”

Section: Discussionsupporting

confidence: 62%

“…An intercomparison of the reference spectra of each species was performed to interrogate the database (14,16). The "start identification" function in the MALDI Biotyper allows the identification of each specimen to be tested, and the results were presented as log score values.…”

Section: Arthropodsmentioning

confidence: 99%

“…In 2011, a blind test in which 111 wild specimens were compared to the database profiles of Culicoides species showed that MALDI-TOF MS can differentiate species of Culicoides biting midges collected in the field (13). More recently, a MALDI-TOF MS study of seven ticks reported that whole ticks or body parts, excluding the legs, generate spectra that are sufficient for species identification (14).…”

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confidence: 99%

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Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors

et al. 2013

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A method for rapid species identification of ticks may help clinicians predict the disease outcomes of patients with tick bites and may inform the decision as to whether to administer postexposure prophylactic antibiotic treatment. We aimed to establish a matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) spectrum database based on the analysis of the legs of six tick vectors: Amblyomma variegatum, Rhipicephalus sanguineus, Hyalomma marginatum rufipes, Ixodes ricinus, Dermacentor marginatus, and Dermacentor reticulatus. A blind test was performed on a trial set of ticks to identify specimens of each species. Subsequently, we used MALDI-TOF MS to identify ticks obtained from the wild or removed from patients. The latter tick samples were also identified by 12S ribosomal DNA (rDNA) sequencing and were tested for bacterial infections. Ticks obtained from the wild or removed from patients (R. sanguineus, I. ricinus, and D. marginatus) were accurately identified using MALDI-TOF MS, with the exception of those ticks for which no spectra were available in the database. Furthermore, one damaged specimen was correctly identified as I. ricinus, a vector of Lyme disease, using MALDI-TOF MS only. Six of the 14 ticks removed from patients were found to be infected by pathogens that included Rickettsia, Anaplasma, and Borrelia spp. MALDI-TOF MS appears to be an effective tool for the rapid identification of tick vectors that requires no previous expertise in tick identification. The benefits for clinicians include the more targeted surveillance of patients for symptoms of potentially transmitted diseases and the ability to make more informed decisions as to whether to administer postexposure prophylactic treatment.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 62%

Section: Arthropodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors

et al. 2013

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“…Additionally, this proteomic approach has been used for the identification of metazoans, such as fish species (33), plants (lentil varieties) (34), ticks (35,36), and insects, such as Drosophila fruit flies (37,38) and mosquitoes (39,40).…”

mentioning

confidence: 99%

Comparison of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal

et al. 2015

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Biting midges of the genus Culicoides are implicated as vectors for a wide variety of pathogens. The morphological identification of these arthropods may be difficult because of a lack of detailed investigation of taxonomy for this species in Africa. However, matrix-assisted laser desorption ionization؊time of flight mass spectrometry (MALDI-TOF MS) profiling is efficient for arthropod identification at the species level. This study established a spectrum database of Culicoides spp. from Senegal using MALDI-TOF. Identification of Culicoides insects to the species level before mass spectrometry was performed on the basis of morphological characters. MALDI-TOF MS reference spectra were determined for 437 field-caught Culicoides of 10 species. The protein profiles of all tested Culicoides revealed several peaks with mass ranges of 2 to 20 kDa. In a validation study, 72 Culicoides specimens in the target species were correctly identified at the species level with a similarity of 95 to 99.9%. Four Culicoides protein profiles were misidentified. Nevertheless, six SuperSpectra (C. imicola, C. enderleini, C. oxystoma, C. kingi, C. magnus, and C. fulvithorax) were created. Abdomens of midges were used to amplify and sequence a portion of the mitochondrial cytochrome oxidase I gene (COI). The results obtained using the MALDI-TOF MS method were consistent with the morphological identification and similar to the genetic identification. Protein profiling using MALDI-TOF is an efficient approach for the identification of Culicoides spp., and it is economically advantageous for approaches that require detailed and quantitative information of vector species that are collected in field. The database of African Culicoides MS spectra created is the first database in Africa. The COI sequences of five Culicoides species that were previously noncharacterized using molecular methods were deposited in GenBank. Biting midges of the genus Culicoides are among the smallest hematophagous insects, and they can be a nuisance to humans and animals (1). These insects are implicated as vectors for a wide variety of viruses, such as bluetongue virus (2), African horse sickness virus (3), epizootic hemorrhagic disease virus (4), the Schmallenberg virus that recently emerged in Europe (5-7), Toggenburg orbivirus (8), and the Oropouche virus (9), which is the only human virus transmitted by Culicoides. Additionally, biting midges are involved in the transmission of various human filarial parasites, such as Mansonella perstans (10-13), Mansonella streptocerca, Mansonella ozzardi, and Mansonella rodhaini (14). Recently, Leishmania spp. and Leishmania infantum were detected in Culicoides imicola and Culicoides spp. (15).Vector identification is an important step in the epidemiology of vector-borne diseases. Information on the major vector species may provide a clearer indication of the geographic distribution of the disease or its potential distribution, the location of danger points for high risks of contact with the vector, and access to alternative...

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Criteria for Development of MALDI‐TOF Mass Spectral Database

Kostrzewa

Maier

2017

MALDI‐TOF and Tandem MS for Clinical Microbiology

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Species determination and characterization of developmental stages of ticks by whole-animal matrix-assisted laser desorption/ionization mass spectrometry

Cited by 83 publications

References 27 publications

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors

Comparison of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal

Criteria for Development of MALDI‐TOF Mass Spectral Database

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