Species determination using the red blood cells morphometry in domestic animals

Abstract

Aim:This investigation is placed in the context of continuity of a preliminary study already published; it was conducted in cattle, sheep, goats, horses, and dogs; the main aim is to reveal and develop criteria for the animal species determination based on the morphometric parameters of red blood cells.Materials and Methods:Blood samples were taken from the jugular vein; and the smears were confectioned on slides immediately after the blood collection and stained according to the May-Gründwald Giemsa method. For the morphometric study, three parameters were considered which are: The diameter, the circumference, and the surface of erythrocytes; and measurements were achieved using the OPTIKATM Vision Pro software. Statistical analysis was performed by both analysis of variances and Student’s t analytical tests.Results:The recorded data showed that the three morphometric parameters of red blood cells are higher in dogs followed, respectively, by those of horses, cattle, and sheep, whereas, the goats have the lowest ones. In addition, the obtained results allowed us to propose new reference values for the circumference and the surface of erythrocyte in considered species.Conclusion:This investigation permit concluding that from a drop of blood it is possible to characterize the different animal species, taking into account the diameter, the circumference, and the surface of erythrocytes.

A New Method of Assessing Sheep Red Blood Cell Types from Their Morphology

“…In this study, the mean and standard error values for RBC length and breadth were 5.51 ± 0.47 and 4.9 ± 0.42 µm, respectively. A minor difference in the values of the mentioned morphometric parameters in this study compared to Adili et al [3,10] is likely to be related to the different ovine breed and breeding methods. Breed, age, and sex are known to affect the values of morphometric parameters [11,24].…”

“…Detection of RBC morphology is routinely performed by subjective microscopic evaluation, which is difficult and highly dependent on the operator's expertise. Therefore, it is necessary to implement an automated methodology to analyse red blood cell shape changes to support and improve the operator's capability and to expedite measurements [10,22]. Interpretation of RBC morphology should be carried out in conjunction with other quantitative data from the complete blood count [23].…”

“…Therefore, according to the above-mentioned results of this study, most of the investigated morphometric parameters values cannot be compared. The morphometric study of RBCs seems to show that the diameter, circumference and area of RBCs have a significant influence on the determination of the species of domestic animals [10]. So, ovine RBCs are somewhat smaller than most mammalian RBCs [2], they have a diameter of 4.5 µm, a width of 3.2-5 µm and a lifespan of 70-150 days, they do not aggregate or deform as readily as RBCs of other non-ruminant species [3,10].…”

“…Furthermore, changes in RBC morphometric indicators have been detected in certain human [4,7,8] and dog ailments [9]. Morphometric studies of RBCs showed several morphological changes; the most important one is due to age and breed in sheep and goats [10], and also in fish [11]. Buslovskaya et al [12] defined a set of changes of several morphometric and functional parameters of RBCs and white blood cells, characteristic of different adaptive reactions of the chicken organism.…”

Effects of Therapeutic and Toxic Agents on Erythrocytes of Different Species of Animals

“…But temperature has no effect on flexibility of horse, cattle, sheep, goat, and some human erythrocytes indicating that blood viscosity varies with temperature [108]. Diameter, circumference, and surface area are higher in erythrocytes of dog followed by horse, cattle, sheep, and goat in that order [109].…”