Species differentiation of thermotolerant Campylobacters based on distinctive banding patterns obtained by multiplex PCR

Vondrakova, Lucie; Purkrtová, Sabina; Pazlarová, J.; Demnerova, Kateřina

doi:10.17221/267/2014-cjfs

Cited by 1 publication

(1 citation statement)

References 20 publications

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“…Campylobacter jejuni is a microaerophilic food-borne pathogen capable of entering the human gastrointestinal tract (GIT), particularly after consumption of undercooked meat. After reaching the target area, usually the lower GIT, Campylobacter is able to induce the gastroenteritis known as campylobacteriosis, found world-wide (European Food Safety Authority 2019; Bolton 2015; Vondrakova et al 2015). During transfer along the food chain, Campylobacter cells have to overcome the hostile conditions of the external environment, including temperature changes and the presence of reactive oxygen species (ROS) formed in aerobic conditions (Gambino and Cappitelli 2016).…”

Section: Introductionmentioning

confidence: 99%

Mutagenic strategies against luxS gene affect the early stage of biofilm formation of Campylobacter jejuni

et al. 2021

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Currently, it is clear that the luxS gene has an impact on the process of bio lm formation in Campylobacter jejuni. However, even within the species naturally occurring strains of Campylobacter lacking the luxS gene exist, which can form bio lms. In order to better understand the genetic determinants and the role of quorum sensing through the LuxS/AI-2 pathway in bio lm formation, a set of mutant/complemented strains of C. jejuni 81-176 were prepared. Additionally, the impact of the mutagenic strategy used against the luxS gene was investigated. Bio lm formation was affected by both the presence and absence of the luxS gene, and by the mutagenic strategy used. Analysis by CLSM showed that all mutant strains formed signi cantly less bio lm mass when compared to the wild-type. Interestingly, the deletion mutant (∆luxS) showed a larger decrease in bio lm mass than the substitution (•luxS) and insertional inactivated ( luxS) mutants, even though all the mutant strains lost the ability to produce autoinducer-2 molecules. Moreover, the bio lm of the ∆luxS mutant lacked the characteristic microcolonies observed in all other strains. The complementation of all mutant strains resulted in restored ability to produce AI-2, to form a complex bio lm, and to develop microcolonies at the level of the wild-type.

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Section: Introductionmentioning

confidence: 99%