Species diversity of the genus Osmundea (Ceramiales, Rhodophyta) in the Macaronesian region

Abstract

Species diversity within the genus Osmundea in the Macaronesian region was explored by conducting a comprehensive sampling in the Azores, the Canary, and the Madeira archipelagos. Toward identification, all specimens were first observed alive to verify the absence of corps en cerise, a diagnostic character for the genus and morphometric data were measured (thallus length and width, first-order branches length and width, branchlets length and width, cortical cell length and width in surface view, cortical cell length and width in transverse section). Specimens were sequenced for COI-5P (39 specimens) and three species delimitation methods (Generalized Mixed Yule Coalescent, Automatic Barcode Gap Discovery method, and Poisson Tree Processes) were used to assess the threshold between infra- and interspecific relationships. Subsequently, one or several sequences of plastid-encoded large subunit of RuBisCO (21 specimens) per delimited species were generated to assess the phylogenetic relationships among Macaronesian Osmundea. Moreover, for each delineated species, vegetative and reproductive anatomy was thoroughly documented and, when possible, specimens were either assigned to existing taxa or described as novel species. This integrative approach has provided data for (i) the presence of O. oederi, O. pinnatifida, and O. truncata in Macaronesia; (ii) the proposal of two novel species, O. prudhommevanreinei sp. nov. and O. silvae sp. nov.; and (iii) evidence of an additional species referred as "Osmundea sp.1," which is a sister taxon of O. hybrida.

Analysis of chloroplast genomes and a supermatrix inform reclassification of the Rhodomelaceae (Rhodophyta)

“…, Machín‐Sánchez et al. , Rousseau et al. ), Amansieae (Phillips ,b, , Phillips and De Clerck ), Heterocladieae (Phillips et al.…”

“…The Rhodomelaceae is the largest family of the red algae, with 1,054 species and 149 genera recognized (Guiry and Guiry 2017). The number of species is probably underestimated as new taxa are often described when detailed studies using molecular data are performed (e.g., Sherwood et al 2010, Mach ın-S anchez et al 2016, Savoie and Saunders 2016, D ıaz-Tapia et al 2017a. Moreover, there is a large number of synonyms and taxonomic entities of uncertain status, particularly in the most diverse genera such as Polysiphonia and Laurencia (Guiry and Guiry 2017).…”

“…Since the introduction of molecular tools for macroalgal systematics, some taxa of the Rhodomelaceae have been studied in attempts to clarify relationships among genera within the Polysiphonieae (Choi et al 2001, D ıaz-Tapia et al 2017b), Pterosiphonieae (Savoie and Saunders 2016), Bostrychieae (Zuccarello and West 2006), Laurencieae (Nam et al 1994, Martin-Lescanne et al 2010, Cassano et al 2012, Metti et al 2015, Mach ın-S anchez et al 2016, Rousseau et al 2017, Amansieae (Phillips 2002a,b, 2006, Phillips and De Clerck 2005, Heterocladieae and Pleurostichidieae (Phillips 2000).…”

Molecular Assisted Identification Reveals Hidden Red Algae Diversity from the Burica Peninsula, Pacific Panama

“…Both PHYKOS-4617 and PHYKOS-4634 matched the morphological concept of T. cylindrica, and they are resolved with varying levels of support in a clade that includes specimens from the Caribbean where the type was collected. However, the level of sequence variation between the Punta Burica specimens and other specimens in this clade are greater than the intraspecific values that have been found in many studies i.e., [36,37], and although T. cylindrica is assigned to the Punta Burica specimens here, a world-wide reassessment of the species is needed to determine its true distribution. Tricleocarpa cylindrica has been recorded previously from both the Caribbean and Pacific coasts of Central America e.g., [5,38,39].…”

Phylogeography of the Red Algal Laurencia Complex in the Macaronesia Region and Nearby Coastal Areas: Recent Advances and Future Perspectives

“…The following list of species (Table 1) was created from the information included in the monograph on genera Laurencia, Laurenciella, and Palisada by Gil-Rodriguez et al [49], from data in Machín-Sánchez et al [27,36,50], as well as from unpublished results obtained during the research project CGL 2010-14881, entitled "The Laurencia complex in the Atlantic Ocean" funded by the Ministry of Education (Spanish Government), where the two first authors were deeply involved.…”