Species diversity of xerophilic <i>Aspergillus</i> and <i>Penicillium</i> in marine surface waters revealed by isolation using osmophilic medium

Abstract

The species diversity of xerophilic and halophilic fungi distributed in marine surface water was studied at four local sites located in two geographically distant regions in Japan. At each site, 5 -10 samples were collected and isolated using an osmophilic medium. Species identification was conducted based on nucleotide sequence of calmodulin or β-tubulin and morphological characteristics for Aspergillus, Penicillium, and Talaromyces, and on the sequences of rRNA internal transcribed spacer for the other taxa. Overall, 231 strains were isolated from all sites and classified into 85 species belonged to 12 orders and 33 genera. The isolates that showed better mycelial growth than the control (no NaCl added) in the halotolerance test were defined as halophilic fungi, and only 22 species (10 Aspergillus species and 12 Penicillium species) were halophilic. Comparison of the halophilic fungal flora of the two regions revealed that four species common to both regions were isolated for Aspergillus, but no such species were isolated for Penicillium. Given that 15 halophilic species (10 Aspergillus and 5 Penicillium species) are known to be xerophilic species distributed in indoor environments, it can be inferred that indoor xerophilic species are likely to be widely distributed in marine surface water.