Species diversity regarding the presence of proximal tubular progenitor cells of the kidney

The impressive progress of histochemistry over the last 50 years has led to setting up specific and sensitive techniques to describe dynamic events, through the detection of specific molecules in the very place where they exist in live cells. The scientific field where histochemistry has most largely been applied is histopathology, with the aim to identify disease-specific molecular markers or to elucidate the etiopathological mechanisms. Numerous authors did however apply histochemistry to a variety of other research fields; their interests range from the microanatomy of animal and plant organisms to the cellular mechanisms of life. This is especially apparent browsing the contents of the histochemical journals where the articles on subjects other than pathology are the majority; these journals still keep a pivotal role in the field of cell and tissue biology, while being a forum for a diverse range of biologists whose scientific interests expand the research horizon of histochemistry to ever novel subjects. Thus, histochemistry can always receive inspiring stimuli toward a continuous methodological refinement.

Section: Applications Of Histochemistry In the Recent Scientific Literaturementioning

confidence: 99%

Histochemistry as a versatile research toolkit in biological research, not only an applied discipline in pathology

Pellicciari

2018

“…66,67 The presence of progenitor stem cells has been detected in the kidney, 68 cerebral cortex 69 and cerebellum 70 during human embryogenesis. Viable chondrogenic stem cells have been detected in the knee joint loose body, 71 and the ultrastructural features of mesenchymal stem cells after in vitro expansion have been reviewed.…”

Section: Stem Cells and Development Tissue Biology In Human And Animalsmentioning

confidence: 99%

Is there still room for novelty, in histochemical papers?

Pellicciari

2016

Histochemistry continues to be widely applied in biomedical research, being nowadays mostly addressed to detect and locate single molecules or molecular complexes inside cells and tissues, and to relate structural organization and function at the high resolution of the more advanced microscopical techniques. In the attempt to see whether histochemical novelties may be found in the recent literature, the articles published in the European Journal of Histochemistry in the period 2014-2016 have been reviewed. In the majority of the published papers, standardized methods have been preferred by scientists to make their results reliably comparable with the data in the literature, but several papers (approximately one fourth of the published articles) described novel histochemical methods and procedures. It is worth noting that there is a growing interest for minimally-invasive in vivo techniques (magnetic resonance imaging, autofluorescence spectroscopy), which may parallel conventional histochemical analyses to acquire evidence not only on the morphological features of living organs and tissues, but also on their functional, biophysical and molecular characteristics. Thanks to this unceasing methodological refinement, histochemistry will continue to provide innovative applications in the biomedical field.

“…Several papers were aimed at illustrating the tissue organization and protein expression of still poorly described organs from various Vertebrate and Invertebrate species: [42][43][44][45][46][47][48][49][50][51][52][53][54] here, the morphological description of the microanatomical and histological features was paralleled by the detection of specific molecules responsible for the tissue functional characteristics, also as a consequence of seasonal dynamic changes. [55][56][57] The immunolabeling of marker proteins was extensively applied in studies aimed at distinguishing stem/progenitor cells or at elucidating the process of cell differentiation and morphogenesis during pre-and post-natal development in mammals, including humans, [58][59][60][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76] with special attention to the nervous system, 65,66 sensory organs, [67][68][69] skin, 70 lung, 70,71 and the skeletal apparatus. [72][73][74][75] Also in plants, the histochemical d...…”

Section: Histochemistry Of Single Moleculesmentioning

confidence: 99%

Histochemistry today: Detection and location of single molecules

Pellicciari¹

2017

Especially in the latest years, histochemical investigations have progressively been oriented toward the visualization and quantitative assessment of single molecules, thanks to the availability of stains, reactions and procedures allowing to detect in situ proteins, or carboydrates or nucleic acid sequences with high specificity. This is evident from the recent literature, where in the large majority of the published articles immunohistochemistry, lectin histochemistry or fluorescence in situ hybridization were used as experimental methodologies. Since in biomedical research it is crucial to specifically label and localize molecules there, where they exert their structural roles and activities, histochemistry will continue to provide scientists the most appropriate tools for tracing molecular maps suitable for reaching a mechanistic explanation of cell functions in tissues.