Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Vásquez-García, Andrea; Silva, Thaysa dos Santos; Almeida-Queiroz, Sabrina R. de; Godoy, Silvia Helena Seraphin de; Fernandes, Andrezza Maria; Sousa, Ricardo Luiz Moro de; Franzolin, Raul

doi:10.1590/s0100-736x2017000500004

Cited by 15 publications

(11 citation statements)

References 28 publications

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“…The present study showed milk samples contaminated with Staphylococcus aureus (15% cow, 10% buffalo) and with Staphylococcus epidermidis 5% (for each cow and buffalo), this is lower than (15) who found that the most agents isolated from milk samples at four dairy farms in São Paulo State, Brazil, were Staphylococcus epidermidis and Staph. aureus 17% and 15% respectively, and also lower than ( 14) who recorded 44.9% S. aureus from three bovine herds in Egypt.…”

Section: Discussioncontrasting

confidence: 78%

Evaluation of bacterial contaminants and heavy metals in cow and buffalo raw milk sold in Baghdad governorate

Al-Rudha¹,

Khalil²,

Altaai³

2021

IJVS

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The purpose of this study was to investigate bacterial contamination and heavy metal concentrations in 80 samples of raw milk (cow: 40 and buffalo: 40) gathered from local markets in Baghdad, Iraq. The culture results were classified into ten categories: E. coli was 100% in each cows and buffaloes, Enterobacter Spp 23.75% (25% cow, 22.5% buffalo), Pseudomonas Spp 13.75% (15% cow,12.5% buffalo), Klebsiella Spp 15% (17.5% cow, 12.5% buffalo), Staphylococcus aureus 12.5% (15% cow, 10% buffalo), Staph. epidermidis 5% (for each cow and buffalo), Proteus spp. 10% (12.5% cows, 7.5% buffaloes), E. coli O157 15% (25% cow, 5% buffalo), Yersinia enterocolitica 3.75% (5% cow, 2.5% buffalo) and Salmonella 13.75% (25% cow 2.5% buffalo). The averages of heavy metals concentrations in cow milk samples were 0.62±0.25, 0.25±0.22, 0.31±0.20 and 21±2 mg/kg and in buffalo milk samples were 0.60±0.3, 0.33±0.15, 0.27±0.11 and 18±2.5 mg/kg for Lead (Pb), Cadmium (Cd), Copper (Cu) and Nickel (Ni) respectively. The high concentrations of pathogenic bacteria and metals found in the milk products is a sign of inadequate hygiene and sanitation during milking and post-milking operations, as well as excessive levels of heavy metal pollution in the environment which will affect meat and milk produced by these animals.

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Section: Discussioncontrasting

confidence: 78%

Evaluation of bacterial contaminants and heavy metals in cow and buffalo raw milk sold in Baghdad governorate

Al-Rudha¹,

Khalil²,

Altaai³

2021

IJVS

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“…Stenotrophomona s has been previously reported in cases of ruminant mastitis. Twenty buffalo milk samples isolated from subclinical mastitis caused by infection showed that S. maltophilia - induced mastitis accounted for 4% of cases ( 42 ). S. maltophilia was isolated from milk samples from an outbreak of bovine clinical mastitis ( 43 ).…”

Section: Discussionmentioning

confidence: 99%

The Rumen Microbiota Contributes to the Development of Mastitis in Dairy Cows

Mu³

et al. 2022

Microbiol Spectr

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“…Bacteria of the genus Staphylococcus have been considered the main etiological agents associated with cases of SCM [21,22] and CM [23] in buffaloes. However, overall, nonaureus staphylococci (NAS) species are currently the most prevalent pathogens causing SCM, IMI and, rarely, CM in dairy buffaloes [24,25].…”

Section: Discussionmentioning

confidence: 99%

Staphylococcus microti Strains Isolated from an Italian Mediterranean Buffalo Herd

Ambrosio

Nocera

Garofalo

et al. 2023

Animals

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S. microti is a new species among non-aureus staphylococci (NAS) frequently found in bovine milk samples and associated with subclinical mastitis (SCM). The aim of this study was to analyze the presence of S. microti in 200 composite milk samples and 104 milking parlor surface swabs collected at a buffalo farm in Southern Italy to define its presence in milk and a milking parlor environment. The samples were inoculated onto different agar plates, and the isolates were identified by MALDI-TOF MS. The strains identified as S. microti (54/304 samples, 17.8%) were collected, and their purified genomic DNA was subjected to PCR amplification and whole 16S rRNA gene sequencing. Furthermore, their phenotypic resistance profiles were evaluated by a disk diffusion method, and the genotypic characterization of the tetracycline resistance was performed for the tetM and tetK genes by multiplex PCR. Four and forty-seven S. microti isolates from milk samples of lactating animals with subclinical mastitis (SCM) and intramammary infection (IMI), respectively, and three isolates from milking parlor surfaces were recovered. The genomic DNA was purified from the bacterial isolates, and the amplification and sequencing of the 16S gene further supported the proteomic identification as S. microti. No clinical mastitis was detected in the herd during the study period. The antimicrobial susceptibility testing revealed a worrisome 100% resistance to tetracyclines, genotypically mediated by the tetM gene for all strains. This study highlights that S. microti may be commonly isolated from dairy buffalo milk and milking parlor equipment. Its association with SCM or IMI remains to be established.

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Species identification and antimicrobial susceptibility profile of bacteria causing subclinical mastitis in buffalo

Cited by 15 publications

References 28 publications

Evaluation of bacterial contaminants and heavy metals in cow and buffalo raw milk sold in Baghdad governorate

Evaluation of bacterial contaminants and heavy metals in cow and buffalo raw milk sold in Baghdad governorate

The Rumen Microbiota Contributes to the Development of Mastitis in Dairy Cows

Staphylococcus microti Strains Isolated from an Italian Mediterranean Buffalo Herd

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