Species identification in meat products using real-time PCR

Jonker, K. M.; Tilburg, Jeroen J. H. C.; Hägele, Geke; Boer, E de

doi:10.1080/02652030701584041

Cited by 79 publications

(45 citation statements)

References 9 publications

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“…Application of the 4-h pre-enrichment step in the present research enables to provide the results within the same working day. Moreover, the detection limit for the designed 4-h pre-enrichment/real-time PCR protocol amounted to 1 cfu·25 mg −1 , which proved a very high sensitivity of the probe/primers set for the identification of Y. enterocolitica species in raw pork meat (Jonker et al 2008;Kesmen et al 2009;Koppel et al 2011). The very low detection limit of Yersinia strains can be also explained by the fact that in meat there is a very high availability of nutrients, and lack of growth inhibitors in pre-enriched meat samples which might encourage the multiplication of Yersinia to achieve the levels that are detectable within only 4 h (Kesmen et al 2007).…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays, molecular methods based on the sequencing of nucleic acids involving the polymerase chain reaction (PCR) and the real-time polymerase chain reaction (real-time PCR) are used as they are reliable, specific, sensitive and time saving protocols for both qualitative and quantitative detection of various pathogenic microorganisms in meat and meat products (Tanabe et al 2007;Jonker et al 2008). Special attention should be paid to the real-time PCR method which is gaining popularity in different analytical applications in food processing, making it possible to detect various pathogenic bacteria and monitor the gene expression of bacteria caused by industrial procedures (Dooley et al 2004).…”

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confidence: 99%

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Designing a time-effective TaqMan probe-based real-time polymerase chain reaction protocol for the identification of Yersinia enterocolitica in raw pork meat

Stachelska

2017

Acta Vet. Brno

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The aim of this study was to design a time-effective method comprising a short pre-enrichment step in a non-selective broth in combination with the TaqMan probe applied in the real-time polymerase chain reaction to detect Yersinia enterocolitica strains in raw pork meat. The method enabled to detect 1 colony forming unit per 25 mg of Yersinia enterocolitica in pork meat. The specificity and reliability of the method was not diminished by the company of microflora naturally present in meat. The method was found successful to detect pathogenic Yersinia enterocolitica strains in pork meat. It is advised to be used for assessing the microbial risk and for controlling the microbial quality of meat and meat products.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Designing a time-effective TaqMan probe-based real-time polymerase chain reaction protocol for the identification of Yersinia enterocolitica in raw pork meat

Stachelska

2017

Acta Vet. Brno

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“…Some PCR approaches (nuclear or mitochondrial) are PCR-based species-specific repeat (SSR), random amplified polymorphic DNAs (RAPD-PCR) fingerprints [15], species-specific short tandem repeat (STR) [16], multiplex PCR [17], quantitative intra-short interspersed element PCR [18], microsatellites analysis [19], and Real-time PCR [20]. Others are focused on sequencing of specific mitochondrial genes such as (12S, 16S and 18S) rRNA [21][22][23][24][25], the Hypervariable Displacement Loop (D-loop) region [21,25], and the cytochrome-b gene (cyt-b) [25,26].…”

Section: Introductionmentioning

confidence: 99%

Using species-specific repeat and PCR–RFLP in typing of DNA derived from blood of human and animal species

El‐Sayed

Mohamed

Ashry

et al. 2009

Forensic Sci Med Pathol

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Species determination of tissue specimens, including blood, is an important component of forensic analysis to distinguish human from animal remains. DNA markers based on a method of species-specific PCR and amplifying the 359-base pair (bp) fragment of the mitochondrially encoded cytochrome-b gene and then digestion with the TaqI restriction enzyme were developed for detection and discrimination of human, cattle, buffalo, horse, sheep, pig, dog, cat and chicken blood samples. The results reveal that PCR-amplification of the gene encoding the species-specific repeat (SSR) region generated 603 bp in cattle and buffalo, 221 bp in horse, 374 bp in sheep,

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“…If the Ct value is measured in exponential phase, as long as the reagent is not limited to, real-time PCR can accurately and reliably calculate the amount of DNA present in the reaction. There are several types of fluorescence based on chemicals used for the detection of real-time PCR, which can be classified into four types: probe hydrolysis as TaqMan®, probe hairpin as molecule Beacon molecule, probe hybridization labeled with fluorescence (FRET) and the DNA intercalation dye as SYBR® Green and EvaGreen® [46,47].…”

Section: Confirmation Of Pig Species Using Real-time Pcrmentioning

confidence: 99%

Molecular Based Method Using PCR Technology on Porcine Derivative Detection for Halal Authentication

Erwanto¹

2018

Genotyping

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Halal food and pharmaceuticals were taken into account as food and pharmaceutical products permitted to be consumed by Muslim according to Sharia law. Due to the development of science and technology, especially in food and pharmaceutical industry, some industries use non-halal components such as pig derivatives in food and pharmaceutical products to reduce the production cost. Non-halal components added in food and pharmaceutical products are difficult to detect visually due to close similarity between non-halal and halal components present in food and pharmaceutical products. Some of the methods already developed in the laboratory include spectroscopic methods using infrared radiation, Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy, chromatography-based methods, electronic nose, DNA-based and differential scanning calorimetric for pig component analysis. Food and pharmaceutical matrix is very complex to be analyzed; therefore, the signals obtained during chemical and biological analyses are very complex and are difficult to interpret. Even though the spectroscopy-and chromatography-based methods are able to determine the pig derivative component, there are some difficulties for the application in the field of blind samples, caused by the complex matrix of food or pharmaceutical. Among analytical methods, the polymerase chain reaction (PCR) based on the molecular genetic analysis was believed to be highly sensitive, valid and judgable as well as reliable for the analytical instrument. Therefore, this chapter describes some methodologies based on DNA technology such as conventional PCR using universal primer through restriction fragment length polymorphism or specific primer. This chapter also gives detailed information on the application of the real-time PCR using species-specific primer for porcine determination as well as for halal authentication.

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Species identification in meat products using real-time PCR

Cited by 79 publications

References 9 publications

Designing a time-effective TaqMan probe-based real-time polymerase chain reaction protocol for the identification of Yersinia enterocolitica in raw pork meat

Designing a time-effective TaqMan probe-based real-time polymerase chain reaction protocol for the identification of Yersinia enterocolitica in raw pork meat

Using species-specific repeat and PCR–RFLP in typing of DNA derived from blood of human and animal species

Molecular Based Method Using PCR Technology on Porcine Derivative Detection for Halal Authentication

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