Species identification method for marine products of Seriola and related species

Abstract

The complete nucleotide sequences of mitochondrial DNA (mtDNA) from four Seriola spp. (S. quinqueradiata, S. lalandi, S. dumerili, and S. rivoliana) were determined with the aim of developing a species identification analysis method for discriminating between commercially important Seriola spp. and other related species. In addition, the nucleotide sequences of the mitochondrial cytochrome b gene (Cytb) from five related but less expensive species in terms of market value (Seriolella brama, S. caerulea, S. punctata, Hyperoglyphe japonica, and Rachycentron canadum), which are often used as substitutes for Seriola spp., were determined. Restriction enzyme sites were examined by comparing the nucleotide sequences, and species-specific primers were designed for PCR-based restriction fragment length polymorphism (RFLP) analysis. Based on the results of the PCR amplification studies, the four Seriola spp. and the five related species tested could be categorized into three groups according to their PCR product pattern: a 373-bp product from the four Seriola spp., a 513-bp product from three Seriolella spp. and H. japonica, and a 204-bp product from R. canadum. In addition, RFLP analysis of the PCR products was able to differentiate these fish species.

Identification of Puffer Fish of the GenusLagocephalus: L. lunaris, L. spadiceusandL. inermis, Using Multiplex PCR

“…2). The sequence variations of both the 12S rRNA and Cyt b genes have been utilized in the identification of various marine species; for example, fish species of the genus Seriola (Iguchi et al, 2012), species of Cyprinidae fish (Chen et al, 2012) order Lamniformes (Hoelzel, 2001) and penaeid shrimp species (Pascoal et al, 2008).…”

Characterization of Greater Amberjack Microsatellite Markers in Lesser Amberjacks, Yellowtail Jacks, Almaco Jacks, and Banded Rudderfish

“…For aquaculture facilities producing larvae that are subsequently grown for future sale, parentage assignments (Gold et al 2010) can identify broodstock contribution and can be used to maximize mating design and output. Differences in microsatellite allele sizes between species can be used to authenticate the labeling of aquaculture products as they go to market (Iguchi et al 2012). As part of a larger set of markers, these microsatellites can be employed for the mapping of quantitative trait loci and the marker-assisted selection of future broodfish (Liu and Cordes 2004).…”

“…Nuclear-encoded microsatellites are useful markers for elucidating stock structure of "wild" fish (Miller et al 2011) and can contribute to fisheries management decisions (Reiss et al 2009). Microsatellites also are valuable for use in aquaculture programs, as they can serve as tools for parentage assignments (Gold et al 2010), species authentication (Iguchi et al 2012), and marker-assisted selection in "farmed" fish (Liu and Cordes 2004). In this paper, we evaluate the amplification of 31 microsatellites developed from the genomic DNA of greater amberjacks for cross-amplification in lesser amberjacks, yellowtail jacks, almaco jacks, and banded rudderfish.…”

A genetic approach for authentication on morphological differences of cultivated pompano species in Indonesia

“…Applied DNA barcode marker sequences to specific taxonomic organisms are invaluable for understanding species diversity, community ecology, trophic interactions, the evolution of functional traits, and conservation (Kress et al 2015). Complete mitochondrial genome sequences can provide essential data for understanding molecular systematics, population structure, stock evaluation, and conservation genetics (Iguchi et al 2012). The data also helps develop appropriate policies to formulate fisheries resource management strategies, especially T. blochii.…”