Species identification of dermatophytes isolated in China by matrix‐assisted laser desorption ionisation‐time‐of‐flight mass spectrometry

Shao, Jinyan; Wan, Zhe; Li, Ruoyu; Yu, Jin

doi:10.1111/myc.13175

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…Additionally, molecular identification studies have classified T. benhamiae within the T. benhamiae series and other related species, such as T. verrucosum, T. concentricum, and T. erinacei [62]. This classification has been supported by genetic diversity analyses, which have revealed the intraspecies variability and mating behavior of A. benhamiae, indicating its close relationship with other species within the T. benhamiae series [60].…”

Section: Discussionmentioning

confidence: 86%

A Current Diagnostic and Therapeutic Challenge: Tinea Capitis

Ion,

Popa,

Porumb-Andrese

et al. 2024

JCM

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Tinea capitis is a dermatophyte scalp infection with a marked prevalence among the pediatric population. However, in the last few years, its epidemiology has changed due to increasing population migration worldwide. Host-specific and environmental factors contribute to the pathogenesis of tinea capitis. Clinically, tinea capitis may present as a subtle hair loss accompanied by scalp scaling, alopecia with scaly patches, or alopecia with black dots. A more severe form of tinea capitis is represented by kerion celsi, which clinically presents as a tender plaque covered by pustules and crusts. If left untreated, this dermatophytic infection may resolve with permanent scarring and alopecia. The pathological changes found in tinea capitis are reflected by a spectrum of clinical changes. Zoophilic infections typically prompt an extensive inflammatory reaction, while anthropophilic dermatophytoses often lack inflammation and result in more persistent lesions. Tinea capitis typically requires systemic antifungal therapy. Griseofulvin, terbinafine, itraconazole, and fluconazole are the main antifungal agents used. Currently, the duration of antifungal therapy varies based on the clinical presentation and type of dermatophyte involved. Through the reported cases and literature review, we aim to emphasize the importance of the early recognition of atypical variants of tinea capitis in immunocompetent children for the prompt initiation of systemic antifungal therapy, minimizing the need for prolonged treatment. Additionally, we emphasize the importance of regular laboratory testing during systemic antifungal therapy, particularly liver enzyme tests, to prevent adverse events, especially in cases requiring long-term treatment.

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Section: Discussionmentioning

confidence: 86%

A Current Diagnostic and Therapeutic Challenge: Tinea Capitis

Ion,

Popa,

Porumb-Andrese

et al. 2024

JCM

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“…In addition, we found the MSP dendrogram cannot differentiate species in the S. apiospermum complex. Shao et al (2020) also found that the MSP dendrogram was difficult to distinguish species in the Trichophyton mentagrophytes series. These results indicated discrimination of close species in some species complex by MSP dendrogram is difficult.…”

Section: Discussionmentioning

confidence: 98%

Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species

et al. 2022

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Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful microorganism identification tool. Research on MALDI-TOF MS identification of rare filamentous fungi is still lacking. This study aimed to evaluate the performance of MALDI-TOF MS in the identification of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus species. Sabouraud broth cultivation and formic acid/acetonitrile protein extraction were used for MALDI-TOF MS identification by a Bruker Biotyper system. An in-house database containing 29 isolates of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus spp. was constructed. A total of 52 clinical isolates were identified using the Bruker Filamentous Fungi Library v1.0 (FFL v1.0) alone, and Filamentous Fungi Library v1.0 plus the in-house library, respectively. The mass spectrum profile (MSP) dendrograms of the 28 Scedosporium isolates, 26 Acremonium-like isolates, and 27 Scopulariopsis and Microascus isolates were constructed by MALDI Biotyper OC 4.0 software, respectively. The correct species identification rate significantly improved when using the combined databases compared with that when using FFL v1.0 alone (Scedosporium spp., 75% versus 0%; Acremonium-like spp., 100% versus 0%; Scopulariopsis and Microascus spp., 100% versus 62.5%). The MSP dendrograms differentiated Acremonium-like species, Scopulariopsis and Microascus species clearly, but cannot distinguish species in the Scedosporium apiospermum complex. In conclusion, with an expanded database, MALDI-TOF MS is an effective tool for the identification of Scedosporium, Acremonium-like, Scopulariopsis, and Microascus species.

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Mini-Review: The Diagnostic Methods of Tinea Capitis

Wei

Qiao

2023

Mycopathologia

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Species identification of dermatophytes isolated in China by matrix‐assisted laser desorption ionisation‐time‐of‐flight mass spectrometry

Cited by 5 publications

References 30 publications

A Current Diagnostic and Therapeutic Challenge: Tinea Capitis

A Current Diagnostic and Therapeutic Challenge: Tinea Capitis

Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Scedosporium, Acremonium-Like, Scopulariopsis, and Microascus Species

Mini-Review: The Diagnostic Methods of Tinea Capitis

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