Species Identification of Pufferfish Products Using RAPD Analysis

Abstract

A simple and rapid method was developed to identify the source species of pufferfish products. Randomly amplified polymorphic DNA RAPD analysis was applied to identify 8 species of pufferfish. Commercial kits were used for DNA extraction and amplification. Simultaneous identification was possible by polyacrylamide gel electrophoresis of PCR products. Two primers were chosen based on the result of pre-examination with 40 primers, and the PCR conditions were optimized. Characteristic RAPD patterns were obtained for each pufferfish species. The developed method was applied to identify the source species of 26 pufferfish products. The results suggest that the developed method would be useful for verification of the labeled species of pufferfish products.

Underwater Undulating Propulsion Biomimetic Robots: A Review

“…The most representative diodontiform fish species is the puffer fish. The puffer fish utilizes fluctuation of the pectoral fins on both sides of its body to propel [ 52 ]. Compared with rajiform fish species, the pectoral fins of diodontiform fish are smaller; thus, the traveling speed is slower.…”