Species-specific and differential expression of BSP-5 and other BSP variants in normozoospermic and asthenozoospermic buffalo (Bubalus bubalis) and cattle (Bos taurus) seminal plasma

Divyashree, Bannur C.; Roy, Sudhir C.

doi:10.1016/j.theriogenology.2017.10.014

Cited by 9 publications

(10 citation statements)

References 28 publications

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“…Thus, fertility was found to be correlated with sperm motility (Zhang et al 1998;Januskauskas et al 2003), and with viability and chromatin integrity (Januskauskas et al 2003;Nongbua et al 2014). The differences in expression of BSP proteins in normospermic and asthenospermic bulls were shown to be related to sperm motility (Divyashree and Roy, 2018).…”

Section: Introductionmentioning

confidence: 97%

Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity

Nongbua

Al-Essawe

Edman³

et al. 2018

Systems Biology in Reproductive Medicine

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Section: Introductionmentioning

confidence: 97%

Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity

Nongbua

Al-Essawe

Edman³

et al. 2018

Systems Biology in Reproductive Medicine

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“…At fertilization, these proteins have been shown to play a role in removing membrane phospholipids and cholesterol, initiating sperm capacitation (Manjunath & Thérien, ; Thérien et al, ). Furthermore, BSP5 was over‐expressed in both cattle and buffaloes with increased sperm motility (Divyashree & Roy, ). In our study, BSP5 was expressed in greater protein abundance in cattle.…”

Section: Discussionmentioning

confidence: 99%

Cross comparison of seminal plasma proteins from cattle and buffalo (Bubalus bubalis)

Codognoto

Yamada

Schmith

et al. 2019

Reprod Domestic Animals

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The objective of this study was to evaluate seminal plasma proteins from cattle and buffalo (Bubalus bubalis), to identify differences between related species. Sixteen buffaloes and 16 cattle between 30 and 60 months of age were used. Semen collection was performed by electroejaculation, followed by macroscopic and microscopic subjective analyses. After analysis, the samples were centrifuged at 800 g for 10 min, and the supernatant (seminal plasma) was recentrifuged at 10,000 g for 30 min at 4°C.The total protein concentration was determined by the Bradford method, and the proteins were digested in solution for mass spectrometry (nLC-MS/MS). Multivariate statistical analysis was used to evaluate the proteomics results by non-hierarchical clustering the considering exponentially modified protein abundance index (emPAI).Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were used for clustering. Proteomics identified 78 proteins, and multivariate analysis showed 4 that were over-expressed in buffaloes (cystatin C, prosaposin, peptide YY and keratin type II cytoskeletal 5) and 9 in cattle (spermadhesin-1, seminal plasma protein PDC-109, ribonuclease 4, metalloproteinase inhibitor 2, acrosin inhibitor 1, seminal ribonuclease, C-type natriuretic peptide, angiogenin-1 and osteopontin). Among the proteins identified in seminal plasma, the C-type natriuretic peptide and metalloproteinase inhibitors were described for the first time in buffaloes. Some protease inhibitors were found over-expressed in buffaloes, and important proteins in seminal plasma of cattle were not identified or were found at lower expression levels in buffaloes, which can contribute to reproductive performance in this species. K E Y W O R D Smale, proteomics, semen, spectrometry-mass | INTRODUC TI ONBuffaloes are marked by rusticity and were the first species inserted in regions in which cattle farming was infeasible due to climatic and/ or geographical conditions. Posteriorly, with the improvement in productive techniques associated with species characteristics such as high adaptability and fertility, potentiality and productive longevity, feed conversion efficiency, meat and milk production capacity, traction strength, resistance to parasitism and development of infectious diseases, they were reintroduced into areas already devoted to cattle All authors have contributed equally to this work.

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“…The major aliquot of semen was centrifuged to separate sperm pellet and seminal plasma as described earlier (Divyashree & Roy, 2018). The sperm pellet obtained was subjected to sonication followed by sucrose gradient centrifugation to separate sperm head and tail according to the method of O'Brien & Bellve (1980) as modified by Carrera et al (1994).…”

Section: Separation Of Buffalo Sperm Head and Tailmentioning

confidence: 99%

“…Proteins separated by SDS-PAGE gels were transferred on to the Immobilon-P PVDF membrane (pore size, 0.45 mm) as described earlier (Divyashree & Roy, 2018). The blots were incubated with goat anti-human Tektin-2 and SPAG6 polyclonal antibodies @ 2.6 μg/mL and 2.0 μg/mL, respectively (sc-160104, sc-165528; Santa Cruz Biotech, Dallas, Texas, US); mouse monoclonal antiphosphotyrosine antibody @ 1.0 μg/mL (P1869, Sigma-Aldrich, St. Louis, MO, USA) for 2 hours at room temperature.…”

Section: Sds-page and Western Blot For Detection Of Tektin-2 Spag6 And Tyrosine Phosphorylated Proteinsmentioning

confidence: 99%

ASTHENOZOOSPERMIC BUFFALO (Bubalus bubalis) SPERM TAIL IS ASSOCIATED WITH LOWER EXPRESSION OF TEKTIN-2 AND SPAG6 AND HIGHER EXPRESSION OF SPECIFIC TYROSINE PHOSPHORYLATED PROTEINS

Divyashree¹,

Physiology²,

Roy³

et al. 2019

JEBAS

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Study of motility-associated proteins is a direct approach to decode the underlying mechanisms of the motility-regulation pathway in a mammalian spermatozoon. In this aspect, attempts were made to characterize and study the expression pattern of two candidate motility-associated proteins of mammalian sperm such as Tektin-2 and SPAG6 and post-translationally modified tyrosine phosphorylated proteins between the two defined groups of buffaloes: normozoospermic (NS) and asthenozoospermic (AS). 1-D Western blot demonstrated three Tektin-2 immunoreactive bands (95.3, 55.7 and 33.0 kDa) in buffalo sperm tails. Of these, the intensity of 95.3 kDa band reduced significantly (P ≤ 0.05) in sperm tail of AS group as compared to that of NS group. Similarly, the sperm ta il demonstrated a single SPAG6-immnunoreactive band of 31.6 kDa that reduced significantly (P ≤ 0.05) in sperm tail of AS group as compared to that of NS group. Both AS and NS group sperm tail demonstrated at least five tyrosine phosphorylated proteins (89.5, 53.3, 44.5, 30.9 and 16.7 kDa). Of these, the intensity of 44.5 kDa band was significantly higher (P ≤ 0.05) in AS group as compared to that of NS group. Interestingly, within the AS group, degree of expression of 53.3 kDa tyrosine phosphorylated protein was bull-specific. The findings of the present study have demonstrated that AS group of buffalo bulls are associated with lower expression of Tektin-2 and SPAG6 and higher expression of specific tyrosine phosphorylated proteins.

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Species-specific and differential expression of BSP-5 and other BSP variants in normozoospermic and asthenozoospermic buffalo (Bubalus bubalis) and cattle (Bos taurus) seminal plasma

Cited by 9 publications

References 28 publications

Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity

Adding bovine seminal plasma prior to freezing improves post-thaw bull sperm kinematics but decreases mitochondrial activity

Cross comparison of seminal plasma proteins from cattle and buffalo (Bubalus bubalis)

ASTHENOZOOSPERMIC BUFFALO (Bubalus bubalis) SPERM TAIL IS ASSOCIATED WITH LOWER EXPRESSION OF TEKTIN-2 AND SPAG6 AND HIGHER EXPRESSION OF SPECIFIC TYROSINE PHOSPHORYLATED PROTEINS

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