1998
DOI: 10.3109/15419069809109149
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Species-Specific Association of the Cell-Aggregation Molecule Mediates Recognition in Marine Sponges

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Cited by 16 publications
(9 citation statements)
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“…When AFs purified from 3 different sponge species were coupled to colored beads and used in an overlay assay with the same AFs bound to a membrane, a striking specificity was observed (Popescu and Misevic, 1997). In a similar approach (Jarchow and Burger, 1999), purified AFs of different sponge species were dotted onto a membrane and overlaid with the corresponding biotinylated AFs. When the protein in the factors had been biotinylated, only homoassociation was observed with the exception of H. panicea AF, which also bound weakly to S. ficus AF.…”
Section: Sponge Aggregation Factorsmentioning
confidence: 99%
See 1 more Smart Citation
“…When AFs purified from 3 different sponge species were coupled to colored beads and used in an overlay assay with the same AFs bound to a membrane, a striking specificity was observed (Popescu and Misevic, 1997). In a similar approach (Jarchow and Burger, 1999), purified AFs of different sponge species were dotted onto a membrane and overlaid with the corresponding biotinylated AFs. When the protein in the factors had been biotinylated, only homoassociation was observed with the exception of H. panicea AF, which also bound weakly to S. ficus AF.…”
Section: Sponge Aggregation Factorsmentioning
confidence: 99%
“…There is no doubt about the ability of sponge AFs to aggregate in a species-specific way formaldehyde-fixed cells (Burger and Jumblatt, 1977;Jumblatt et al, 1980) and cells kept at low temperatures where cell metabolism is inhibited (Humphreys, 1963;Moscona, 1963). Species-specificity has even been claimed in cell-free systems (Jarchow and Burger, 1999;Popescu and Misevic, 1997), suggesting that sponge cell aggregation might be the result of the passive interaction of chemi-cal structures, without any participation of the active metabolism of the cells.…”
Section: Allogeneic Reactions In Sponges: a Possible Link Between Celmentioning
confidence: 99%
“…AFs (1 mg of protein/ml) were biotinylated with biotin-6-aminocaproic acid-hydroxysuccinimidester (Fluka) as described (Jarchow and Burger, 1998). Glycans obtained after pronase digestion were labeled under the same conditions, except that a large molar excess of the reactive agent (at least 20-fold) was added for amino-terminal labeling of the remaining amino acid residue.…”
Section: Sponges and Preparation Ofmentioning
confidence: 99%
“…1C). This model has been demonstrated using cell-free techniques that include bead aggregation experiments (12,16,22), membrane blot assays (23, 24), and force spectroscopy studies (12,25). Recent data have shown that the binding forces recorded between g200 from the same species were significantly stronger than those observed between g200 from different species (12, 13), indicating that the measurement of adhesive forces mediated by surface glycans may contribute to the study of molecular systems having determinant implications in cellular recognition.…”
mentioning
confidence: 99%