Species-specific detection and quantification of scyphomedusae in Jiaozhou Bay, China, using a quantitative real-time PCR assay

Wang, Jianyan; Mi, Tiezhu; Yu, Zhigang; Wang, Guoshan; Yang, Jing; Zhen, Yu

doi:10.1007/s00343-020-0160-0

Cited by 3 publications

(1 citation statement)

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“…Environmental DNA (eDNA) techniques, which detect the DNA fragments directly extracted from the environment including DNA from living cells shed by organisms and extracellular DNA freed from cells after an organism dies (Nielsen et al., 2007 ), have emerged as a potential powerful tool to assess aquatic community structures in a specified area. A species‐specific quantitative PCR method utilizes targeted primers focusing on the detection of a few targeted species (e.g., Bolte et al., 2021 ; Gaynor et al., 2017 ; Minamoto et al., 2017 ; Ogata et al., 2021 ; Sathirapongsasuti et al., 2021 ; Takahashi et al., 2020 ; Takasu et al., 2019 ; Wang et al., 2021 ). In contrast, the detection of multiple species can be undertaken through general metabarcoding using conserved primers (e.g., Alexander et al., 2020 ; Ames et al., 2021 ; Beentjes et al., 2022 ; Clark et al., 2020 ; Euclide et al., 2021 ; Pappalardo et al., 2021 ), which is advantageous in biodiversity surveys.…”

Section: Introductionmentioning

confidence: 99%

Application of environmental DNA metabarcoding and quantitative PCR to detect blooming jellyfish in a temperate bay of northern China

Peng,

Wang,

et al. 2023

Ecology and Evolution

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Frequently occurring jellyfish blooms have severe impacts on the socioeconomics of coastal areas, which stress the importance of early detection and assessments of blooming jellyfish taxa. Environmental DNA (eDNA) techniques (quantitative PCR and eDNA metabarcoding) have the advantage of high sensitivity and are an emerging powerful tool for investigations of target species. However, a comprehensive analysis of the biodiversity and biomass of jellyfish taxa in the target area by combining the two eDNA techniques is still lacking. Here, we developed eDNA metabarcoding and quantitative PCR for the detection and assessment of jellyfish taxa in the temperate Yantai Sishili Bay (YSB) and estimated the spatial distribution of Aurelia coerulea. Species‐specific quantitative PCR assays targeting the mitochondrial cytochrome c oxidase subunit I gene of A. coerulea were developed. Additionally, eDNA metabarcoding based on the mitochondrial 16S rDNA sequences identified six jellyfish species in YSB. Moreover, our results indicate that A. coerulea aggregations were more likely to occur in the inner part of the bay than in the outer part, and they gathered in the bottom layer of seawater rather than in the surface layer. Our results demonstrate the potential of two eDNA techniques in jellyfish biomass investigation and jellyfish taxa detection. These eDNA techniques may contribute to the discovery of jellyfish aggregation so as to achieve early warning of large‐scale jellyfish blooms in coastal areas.

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