2016
DOI: 10.1016/j.jviromet.2016.07.024
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Species-specific differentiation of variola, monkeypox, and varicella-zoster viruses by multiplex real-time PCR assay

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Cited by 101 publications
(89 citation statements)
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“…Samples for MPX virus (MPXV) PCR testing were taken from blood, throat, anal region, skin vesicles and urine [ 6 , 7 ]. See the Supplement for details concerning diagnostic tests.…”
Section: Clinical Case Descriptionmentioning
confidence: 99%
“…Samples for MPX virus (MPXV) PCR testing were taken from blood, throat, anal region, skin vesicles and urine [ 6 , 7 ]. See the Supplement for details concerning diagnostic tests.…”
Section: Clinical Case Descriptionmentioning
confidence: 99%
“…Various RT-PCR combinations were used in the design of the methods for the detection and species-specific identification of orthopoxviruses. A large set of methods providing the detection of orthopoxviruses have been elaborated so far; mainly, they utilize TaqMan and LightCycler (Roche Indianapolis, IN, USA) variants of RT-PCR [ 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 ]. Several of these assays have undergone review by regulatory agencies for diagnostic use.…”
Section: Methods For Species-specific Diagnosis Of Orthopoxvirusesmentioning
confidence: 99%
“…In the case of orthopoxviruses pathogenic for humans, test kits that provide genus-specific DNA identification for an assayed virus with concurrent species-specific differentiation are a priority. The VECTOR team was the first to elaborate such methods based on classical multiplex PCR [ 52 , 53 ] and multiplex real-time PCR [ 54 - 58 ].…”
Section: Species-specific Dna Diagnostics Of Orthopoxvirusesmentioning
confidence: 99%