“…Various RT-PCR combinations were used in the design of the methods for the detection and species-specific identification of orthopoxviruses. A large set of methods providing the detection of orthopoxviruses have been elaborated so far; mainly, they utilize TaqMan and LightCycler (Roche Indianapolis, IN, USA) variants of RT-PCR [ 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 ]. Several of these assays have undergone review by regulatory agencies for diagnostic use.…”