Species-specific phytoplankton growth rates via diel DNA synthesis cycles. II DNA quantification and model verification in the dino-flagellate Heterocapsa triquetra

Chang, Jeng Kuei; Carpenter, E. J.

doi:10.3354/meps044287

Cited by 44 publications

(34 citation statements)

References 14 publications

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“…(2) assumes a constant, known t d . Though there are no direct estimates of t d for Heterocapsa triquetra in the field, a t d value can be approximated from laboratory studies of H. triquetra grown at an average daily temperature of 13.5°C (Chang & Carpenter 1988). In these studies, both stationary and exponentially growing cells were stained with a DNA-specific fluorescent dye and the changes in DNA content followed using a sensitive microfluorometry system.…”

Section: Methodsmentioning

confidence: 99%

“…(2) integrated the MI values between 07:00 h one morning and 05:00 h the next morning to reflect any influence of the previous day's light levels on the following night's cell division rate. The maximum growth rates were also estimated by calculating daily water temperatures and a plot of the maximum growth rates of Heterocapsa triquetra at different temperatures derived from non-nutrient limited culture studies (Braarud & Pappas 1951, Yamochi 1984, Anderson & Stolzenbach 1985, Chang & Carpenter 1988.…”

Section: Methodsmentioning

confidence: 99%

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Effect of diel and interday variations in light on the cell division pattern and in situ growth rates of the bloom-forming dinoflagellate Heterocapsa triquetra

Litaker¹,

E.²,

Rhyne³

et al. 2002

Mar. Ecol. Prog. Ser.

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Heterocapsa triquetra is an important bloom-forming dinoflagellate found in estuaries and nearshore regions worldwide. In an initial time-intensive study, the shallow, tidally mixed Newport River estuary, North Carolina, USA, was sampled from a fixed point located in the middle of the estuary every 2 h for 2 wk during the development of an H. triquetra bloom. The objective of this study was to investigate how short-term, high-frequency changes in temperature, light and salinity affected diel and interday cell division patterns and in situ growth rates of H. triquetra. During this study, phytoplankton samples were preserved in buffered formaldehyde and mitotic indices determined by acridine orange staining. The diel division pattern showed a nocturnal maximum between 23:00 and 05:00 h with reduced division during the day, a pattern characteristic of most dinoflagellates. The relative proportion of binucleate cells present during the day was influenced by interday variations in total irradiance, increasing during 2 of the 3 periods when there were 3 or more consecutive high light days (> 28 E m -2 d -1). Approximately 40% of the overall variation in interday division rates could be accounted for by differences in daily irradiance. The interday light differences were largely due to well-developed atmospheric frontal systems that brought increased cloud cover to the study area at regular 3 to 4 d intervals. The initial study, however, was of insufficient length to determine if the transient day-to-day light limitation could significantly affect seasonal bloom formation. A second longer-term, spatially intensive study was therefore undertaken to assess the relative importance of the incident light levels and nutrient inputs in controlling H. triquetra bloom initiation. During the second study, the estuary was monitored for photosynthetically active radiation (PAR), salinity, temperature, inorganic nutrients and cell densities of H. triquetra at 9 locations every wk from 23 December 1997 to 27 March 1998. Maximal H. triquetra bloom formation occurred during a 2 wk period when daily incident light levels were at or near the annual low. This suggested that H. triquetra is well adapted for utilizing low light levels and that variation in in situ growth rates in response to daily changes in PAR had little effect on bloom development. Instead, bloom initiation began with inputs of nitrogen-rich water following a runoff event, indicating that nutrient inputs are much more important in controlling bloom development than is light.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effect of diel and interday variations in light on the cell division pattern and in situ growth rates of the bloom-forming dinoflagellate Heterocapsa triquetra

Litaker¹,

E.²,

Rhyne³

et al. 2002

Mar. Ecol. Prog. Ser.

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“…H. triquetra has a broad temperature growth optimum, with rates > 0.45 d -1 observed from 15 to 26°C, and maximal rates of between 0.55 and 0.69 d -1 occurring at 19 to 20°C (Braarud & Pappas 1951, Yamochi 1984, Chang & Carpenter 1988. However, below 15°C, maximal H. triquetra growth rates decline rapidly from 0.4 d -1 at 15°C, to 0.1 d -1 at 10°C.…”

Section: Low Temperatures Impose Growth Limitation Onmentioning

confidence: 99%

Seasonal niche strategy of the bloom-forming dinoflagellate Heterocapsa triquetra

Litaker¹,

Tester²,

Duke³

et al. 2002

Mar. Ecol. Prog. Ser.

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“…Basically the method is an improvement of the frequency of dividing cells method (Braunwarth and Sommer, 1985;Heller, 1977;Smayda, 1975). The theoretical concepts are well-documented (Chang and Carpenter, 1988;Chang and Carpenter, 1990;Chang and Dam, 1993). Growth rate estimates are based on the DNA frequency distribution traced over a given period of time, usually a 24 h (light/dark) period (Vaulot et al, 1995;Van Bleijswijk and Veldhuis, 1995;Pan and Cembella., 1998; Fig.…”

Section: Growth Ratementioning

confidence: 99%

Application of flow cytometry in marine phytoplankton research: current applications and future perspectives

Veldhuis¹,

Kraay²

2000

Sci. Mar.

169

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SUMMARY: A brief overview is given of current applications of flow cytometry (FCM) in marine phytoplankton research. This paper presents a selection of highlights and various technical and analytical problems we encountered during the past 10 years. In particular, the conversion of the relative values obtained in terms of size and fluorescence applying FCM to quantitative estimates of cell size, pigment concentration, genome size etc., is addressed. The introduction of DNA -cellcycle analysis made easily assessable by flow cytometry has been of great importance, allowing in situ measurement of species specific growth rates. Key questions in ecology such as factors determining the wax and wane of phytoplankton bloom can now be better answered in terms of species specific growth and mortality. Finally, flow cytometry provides detailed information of the physiological status of the individual algal cells. New staining methods enable us to distinguish between viable and non-viable cells and so will help us to elucidate the importance of "automortality" in aquatic ecosystems.

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Species-specific phytoplankton growth rates via diel DNA synthesis cycles. II DNA quantification and model verification in the dino-flagellate Heterocapsa triquetra

Cited by 44 publications

References 14 publications

Effect of diel and interday variations in light on the cell division pattern and in situ growth rates of the bloom-forming dinoflagellate Heterocapsa triquetra

Effect of diel and interday variations in light on the cell division pattern and in situ growth rates of the bloom-forming dinoflagellate Heterocapsa triquetra

Seasonal niche strategy of the bloom-forming dinoflagellate Heterocapsa triquetra

Application of flow cytometry in marine phytoplankton research: current applications and future perspectives

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