Species-specific roles of sulfolipid metabolism in acclimation of photosynthetic microbes to sulfur-starvation stress

Sato, Norihiro; Kamimura, Ryohei; Kaneta, Kodai; Yoshikawa, Misato; Tsuzuki, Mikio

doi:10.1371/journal.pone.0186154

Cited by 13 publications

(8 citation statements)

References 37 publications

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“…These biomolecules included carotenoids, amino acids, lipids, phospholipids, and saccharides. Specific responses observed in photosynthetic organisms during acclimation to Mg and S deprivation include glycogen accumulation (Schwarz & Forchhammer, 2005), changes in amino acids composition (Kiyota, Ikeuchi & Hirai, 2012) as well as lipids (Sato et al, 2017) and degradation of photosynthetic components (i.e., chlorophyll a, PBS and carotenoids) (Jensen & Rachin, 1984;Richaud et al, 2001;Schmidt, Erdle & Köst, 2014). Interestingly, most of these biomolecules were identified with Raman shift contributions, suggesting that our findings are in an agreement with previously established results.…”

Section: Discussionsupporting

confidence: 90%

Characterizing metabolic stress-induced phenotypes ofSynechocystisPCC6803 with Raman spectroscopy

Tanniche

Collakova

Denbow

et al. 2020

PeerJ

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Background During their long evolution, Synechocystis sp. PCC6803 developed a remarkable capacity to acclimate to diverse environmental conditions. In this study, Raman spectroscopy and Raman chemometrics tools (RametrixTM) were employed to investigate the phenotypic changes in response to external stressors and correlate specific Raman bands with their corresponding biomolecules determined with widely used analytical methods. Methods Synechocystis cells were grown in the presence of (i) acetate (7.5–30 mM), (ii) NaCl (50–150 mM) and (iii) limiting levels of MgSO4 (0–62.5 mM) in BG-11 media. Principal component analysis (PCA) and discriminant analysis of PCs (DAPC) were performed with the RametrixTM LITE Toolbox for MATLABⓇ. Next, validation of these models was realized via RametrixTM PRO Toolbox where prediction of accuracy, sensitivity, and specificity for an unknown Raman spectrum was calculated. These analyses were coupled with statistical tests (ANOVA and pairwise comparison) to determine statistically significant changes in the phenotypic responses. Finally, amino acid and fatty acid levels were measured with well-established analytical methods. The obtained data were correlated with previously established Raman bands assigned to these biomolecules. Results Distinguishable clusters representative of phenotypic responses were observed based on the external stimuli (i.e., acetate, NaCl, MgSO4, and controls grown on BG-11 medium) or its concentration when analyzing separately. For all these cases, RametrixTM PRO was able to predict efficiently the corresponding concentration in the culture media for an unknown Raman spectra with accuracy, sensitivity and specificity exceeding random chance. Finally, correlations (R > 0.7) were observed for all amino acids and fatty acids between well-established analytical methods and Raman bands.

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Section: Discussionsupporting

confidence: 90%

Characterizing metabolic stress-induced phenotypes ofSynechocystisPCC6803 with Raman spectroscopy

Tanniche

Collakova

Denbow

et al. 2020

PeerJ

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“…Cells were harvested at the indicated times for the extraction of total lipids, which were then used for separation of individual polar lipids by two-dimensional TLC 57 . A lipid that appeared under −P conditions was identified through mass spectrometric analyses.…”

Section: Methodsmentioning

confidence: 99%

Diacylglyceryl-N,N,N-trimethylhomoserine-dependent lipid remodeling in a green alga, Chlorella kessleri

et al. 2022

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Membrane lipid remodeling contributes to the environmental acclimation of plants. In the green lineage, a betaine lipid, diacylglyceryl-N,N,N-trimethylhomoserine (DGTS), is included exclusively among green algae and nonflowering plants. Here, we show that the green alga Chlorella kessleri synthesizes DGTS under phosphorus-deficient conditions through the eukaryotic pathway via the ER. Simultaneously, phosphatidylcholine and phosphatidylethanolamine, which are similar to DGTS in their zwitterionic properties, are almost completely degraded to release 18.1% cellular phosphorus, and to provide diacylglycerol moieties for a part of DGTS synthesis. This lipid remodeling system that substitutes DGTS for extrachloroplast phospholipids to lower the P-quota operates through the expression induction of the BTA1 gene. Investigation of this lipid remodeling system is necessary in a wide range of lower green plants for a comprehensive understanding of their phosphorus deficiency acclimation strategies.

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“…These nutrients are known to play a vital role in the growth of marine Synecchococcus spp. (Scanlan et al ., ; Sato et al ., ).…”

Section: Resultsmentioning

confidence: 99%

Comparative membrane proteomics reveal contrasting adaptation strategies for coastal and oceanic marine Synechococcus cyanobacteria

Teoh

Shah

Ostrowski

et al. 2020

Environmental Microbiology

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Marine cyanobacteria genus Synechococcus are among the most abundant and widespread primary producers in the open ocean. Synechococcus strains belonging to different clades have adapted distinct strategies for growth and survival across a range of marine conditions. Clades I and IV are prevalent in colder, mesotrophic, coastal waters, while clades II and III prefer warm, oligotrophic open oceans. To gain insight into the cellular resources these unicellular organisms invest in adaptation strategies we performed shotgun membrane proteomics of four Synechococcus spp. strains namely CC9311 (clade I), CC9605 (clade II), WH8102 (clade III) and CC9902 (clade IV). Comparative membrane proteomes analysis demonstrated that CC9902 and WH8102 showed high resource allocation for phosphate uptake, accounting for 44% and 38% of overall transporter protein expression of the species. WH8102 showed high expression of the iron uptake ATP-binding cassette binding protein FutA, suggesting that a high binding affinity for iron is possibly a key adaptation strategy for some strains in oligotrophic ocean environments. One protein annotated as a phosphatase 2c (Sync_2505 and Syncc9902_0387) was highly expressed in the coastal mesotrophic strains CC9311 and CC9902, constituting 14%-16% of total membrane protein, indicating a vital, but undefined function, for strains living in temperate mesotrophic environments.

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Species-specific roles of sulfolipid metabolism in acclimation of photosynthetic microbes to sulfur-starvation stress

Cited by 13 publications

References 37 publications

Characterizing metabolic stress-induced phenotypes ofSynechocystisPCC6803 with Raman spectroscopy

Characterizing metabolic stress-induced phenotypes ofSynechocystisPCC6803 with Raman spectroscopy

Diacylglyceryl-N,N,N-trimethylhomoserine-dependent lipid remodeling in a green alga, Chlorella kessleri

Comparative membrane proteomics reveal contrasting adaptation strategies for coastal and oceanic marine Synechococcus cyanobacteria

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