Specific accumulation and revised structures of acridone alkaloid glucosides in the tips of transformed roots of Ruta graveolens

Кузовкина, И. Н.; Alterman, I. E.; Schneider, Bernd Uwe

doi:10.1016/j.phytochem.2004.03.003

Cited by 66 publications

(35 citation statements)

References 27 publications

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“…This can be achieved in part by extensive use of two-dimensional techniques (Fan 1996), but from work in the biomedical field it is clear that a more complete analysis can usually be achieved by linking the NMR analysis to an in-line chromatographic separation technique, such as liquid chromatography (LC; Lindon et al 1996). In fact LCNMR is a well established analytical approach for natural products (Bringmann et al 1998;Kuzovkina et al 2004) and more complicated schemes, incorporating parallel MS and NMR analysis, have been implemented (Exarchou et al 2003).…”

Section: Metabolomics and Metabolic Flux Analysismentioning

confidence: 99%

NMR analysis of plant nitrogen metabolism

Mesnard¹,

Ratcliffe

2005

Photosynth Res

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The analysis of primary and secondary nitrogen metabolism in plants by nuclear magnetic resonance (NMR) spectroscopy is comprehensively reviewed. NMR is a versatile analytical tool, and the combined use of (1)H, (2)H, (13)C, (14)N and (15)N NMR allows detailed investigation of the acquisition, assimilation and metabolism of nitrogen. The analysis of tissue extracts can be complemented by the in vivo NMR analysis of functioning tissues and cell suspensions, and by the application of solid state NMR techniques. Moreover stable isotope labelling with (2)H-, (13)C- and (15)N-labelled precursors provides direct insight into specific pathways, with the option of both time-course and steady state analysis increasing the potential value of the approach. The scope of the NMR method, and its contribution to studies of plant nitrogen metabolism, are illustrated with a wide range of examples. These include studies of the GS/GOGAT pathway of ammonium assimilation, investigations of the metabolism of glutamate, glycine and other amino acids, and applications to tropane alkaloid metabolism. The continuing development of the NMR technique, together with potential applications in the emerging fields of metabolomics and metabolic flux analysis, leads to the conclusion that NMR will play an increasingly valuable role in the analysis of plant nitrogen metabolism.

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Section: Metabolomics and Metabolic Flux Analysismentioning

confidence: 99%

NMR analysis of plant nitrogen metabolism

Mesnard¹,

Ratcliffe

2005

Photosynth Res

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“…Rue is a native of the Mediterranean region but cultivated throughout Europe and many Asian countries, including China, India, and Japan. The plant contains more than 120 compounds of different classes of natural products such as acridone alkaloids, coumarins, essential oils, flavonoids and furoquinolines [9]. The components of Ruta species are of great interest in medicinal chemistry, as these compounds show a broad range of biological activity and a number of them are used in medicine.…”

Section: Introductionmentioning

confidence: 99%

Protective Effects of Isolated Polyphenolic and Alkaloid Fractions of Ruta graveolens L. on Acute and Chronic Models of Inflammation

et al. 2009

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Ruta graveolens L. (Rutaceae) are traditionally used for the treatment of rheumatism, arthritis and other inflammatory conditions in the traditional medicine of India, were evaluated for their protective effect in acute and chronic models of inflammation. Carrageenan induced rat paw edema and adjuvant induced arthritis were employed as the experimental models of acute and chronic inflammation respectively. Isolated polyphenolic and alkaloid fraction (AFR) from Ruta graveolens and evaluated its anti inflammatory activity in carrageenan induced acute model. AFR with a dose 10 mg/kg showed higher anti inflammatory effect than polyphenols and standard drug diclofenec. AFR significantly decreased the paw edema in arthritic rats. TBARS, COX-2, 5-LOX and MPO level were decreased and the levels of antioxidant enzymes and GSH level were increased on treatment with AFR. The increment in CRP level and ceruloplasmin level observed in arthritic animals were also found to be significantly restored in AFR treated rats. The results demonstrated the potential beneficiary effect of isolated polyphenolic and alkaloid fraction of Ruta graveolens L. on acute and chronic models of inflammation in rats.

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“…In the Siparuna guianensis ('negramina') were found flavonoids (Leitão et al, 2005), essential oils (Fischer et al, 2005) and alkaloids (Simas et al, 2001). Ruta graveolens ('arruda') contains alkaloids (Kuzovkina et al, 2004;Michael, 2005), flavonoids (Saieed et al, 2006), coumarins (Kostova et al, 1999), furanocoumarins (Ekiert and Czygan, 2005), triacylglycerines (Asilbekova, 2001) and glycosides (Chen et al, 2001). For Dorstenia asaroides ('caiá-piá') the phytochemical study showed the presence of furanocoumarins (Cardoso et al, 1999).…”

Section: Catalepsy Testmentioning

confidence: 99%

Preliminary investigation of the central nervous system effects of ‘Tira‐capeta’ (Removing the Devil), a cigarette used by some Quilombolas living in Pantanal Wetlands of Brazil

Rodrigues

Gianfratti

Tabach

et al. 2008

Phytotherapy Research

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During an ethnopharmacological survey carried out among some Quilombolas living in Brazil, 48 plants with possible central nervous system (CNS) action were cited. A mixture of nine plants, known as 'Tira-capeta' (Removing the Devil) cigarette, has been utilized for years as a tonic for the brain. The effects observed after consuming this cigarette are: dizziness, lightness sensation, humor changes, yawns, heavy eyes, hunger, sleep and relaxation. This study aimed to verify the effects of a hydroalcohol extract of 'Tira-capeta' cigarette (ETC), as well as to evaluate the phytochemical profile. The phytochemical screening carried out through characterization reactions, thin layer chromatography and high efficiency liquid chromatography indicated the presence of tannins, phenolic acids, flavonoids, saponins and alkaloids; tannins and phenolic acids being the principal constituents. The pharmacological tests showed that ETC induced a biphasic effect, with intense initial stimulation of the CNS, followed by a general depressor state; decreased the latency for sleeping and increased the total sleeping time (50, 100 and 500 mg/kg), without causing prejudice in motor coordination (doses up to 200 mg/kg); induced catalepsy in mice, verified 10 and 50 min after drug administration (500 mg/kg). Also, no anxiolytic or anxiogenic effects were verified in rats submitted to the elevated plus-maze.

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Specific accumulation and revised structures of acridone alkaloid glucosides in the tips of transformed roots of Ruta graveolens

Cited by 66 publications

References 27 publications

NMR analysis of plant nitrogen metabolism

NMR analysis of plant nitrogen metabolism

Protective Effects of Isolated Polyphenolic and Alkaloid Fractions of Ruta graveolens L. on Acute and Chronic Models of Inflammation

Preliminary investigation of the central nervous system effects of ‘Tira‐capeta’ (Removing the Devil), a cigarette used by some Quilombolas living in Pantanal Wetlands of Brazil

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