Specific Activation of an I-Like Element in Drosophila Interspecific Hybrids

Carnelossi, Elias Alberto Gutierrez; Lerat, Emmanuelle; Henri, Hélène; Martı́nez, Sonia; Carareto, Claudia M. A.; Vieira, Cristina

doi:10.1093/gbe/evu141

Cited by 16 publications

(16 citation statements)

References 58 publications

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“…However, TE expression variation between reciprocal hybrids in the female germ line stresses the necessity of further population studies in order to investigate whether these mobile elements might contribute to post-zygotic reproductive isolation between D. mojavensis and D. arizonae . We have shown that some specific TEs are upregulated in the male germline depending on the source population of males and females, and the direction of the cross53. Thus, sterility in males could be associated with the mobilization of TEs and a transcritome analysis of the male germ line is needed because it could explain why TEs, despite a strong negative selection against deleterious transposition effects, remain successfully active in the male line.…”

Section: Discussionmentioning

confidence: 99%

Identification of misexpressed genetic elements in hybrids between Drosophila-related species

Lopez-Maestre

Carnelossi

Lacroix

et al. 2017

Sci Rep

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Crosses between close species can lead to genomic disorders, often considered to be the cause of hybrid incompatibility, one of the initial steps in the speciation process. How these incompatibilities are established and what are their causes remain unclear. To understand the initiation of hybrid incompatibility, we performed reciprocal crosses between two species of Drosophila (D. mojavensis and D. arizonae) that diverged less than 1 Mya. We performed a genome-wide transcriptomic analysis on ovaries from parental lines and on hybrids from reciprocal crosses. Using an innovative procedure of co-assembling transcriptomes, we show that parental lines differ in the expression of their genes and transposable elements. Reciprocal hybrids presented specific gene categories and few transposable element families misexpressed relative to the parental lines. Because TEs are mainly silenced by piwi-interacting RNAs (piRNAs), we hypothesize that in hybrids the deregulation of specific TE families is due to the absence of such small RNAs. Small RNA sequencing confirmed our hypothesis and we therefore propose that TEs can indeed be major players of genome differentiation and be implicated in the first steps of genomic incompatibilities through small RNA regulation.

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Section: Discussionmentioning

confidence: 99%

Identification of misexpressed genetic elements in hybrids between Drosophila-related species

Lopez-Maestre

Carnelossi

Lacroix

et al. 2017

Sci Rep

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“…(). The TD was conducted combining methods from previous studies (Akkouche et al., ; Carnelossi et al., ; Munroe et al., ; Roy et al., ). First, individual extracted DNA (≈ 75 ng) was used for enzymatic digestion in a total volume of 20 μl, with HindIII enzyme (10 U/μl) and buffer R (Thermo Scientific) for 3 hr at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…The total DNA was extracted from whole adult bodies following the phenol-chloroform protocol described by Minard et al (2015). The TD was conducted combining methods from previous studies (Akkouche et al, 2012;Carnelossi et al, 2014;Munroe et al, 1994;Roy et al, 1999). First, individual extracted DNA (% 75 ng) was used for enzymatic digestion in a total volume of 20 ll, with HindIII enzyme (10 U/ll) and buffer R (Thermo Scientific) for 3 hr at 37°C.…”

Section: Dna Extraction and Td Adapter Ligationmentioning

confidence: 99%

High‐throughput sequencing of transposable element insertions suggests adaptive evolution of the invasive Asian tiger mosquito towards temperate environments

et al. 2017

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Invasive species represent unique opportunities to evaluate the role of local adaptation during colonization of new environments. Among these species, the Asian tiger mosquito, Aedes albopictus, is a threatening vector of several human viral diseases, including dengue and chikungunya, and raises concerns about the Zika fever. Its broad presence in both temperate and tropical environments has been considered the reflection of great "ecological plasticity." However, no study has been conducted to assess the role of adaptive evolution in the ecological success of Ae. albopictus at the molecular level. In the present study, we performed a genomic scan to search for potential signatures of selection leading to local adaptation in one-hundred-forty field-collected mosquitoes from native populations of Vietnam and temperate invasive populations of Europe. High-throughput genotyping of transposable element insertions led to the discovery of more than 120,000 polymorphic loci, which, in their great majority, revealed a virtual absence of structure between the biogeographic areas. Nevertheless, 92 outlier loci showed a high level of differentiation between temperate and tropical populations. The majority of these loci segregate at high insertion frequencies among European populations, indicating that this pattern could have been caused by recent adaptive evolution events in temperate areas. An analysis of the overlapping and neighbouring genes highlighted several candidates, including diapause, lipid and juvenile hormone pathways.

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“…Transposable elements (TEs) may play an outsized role in establishing such incompatibilities because of their ability to rapidly proliferate. Studies of hybridization across diverse systems have revealed complex patterns of increased TE expression and transposition in hybrids [4][5][6][7][8][9][10][11][12]. However, it is not clear if increased TE activity is a general response to hybridization.…”

Section: Introductionmentioning

confidence: 99%

Hybrid dysgenesis in Drosophila virilis results in clusters of mitotic recombination and loss-of-heterozygosity but leaves meiotic recombination unaltered

et al. 2020

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Background: Transposable elements (TEs) are endogenous mutagens and their harmful effects are especially evident in syndromes of hybrid dysgenesis. In Drosophila virilis, hybrid dysgenesis is a syndrome of incomplete gonadal atrophy that occurs when males with multiple active TE families fertilize females that lack active copies of the same families. This has been demonstrated to cause the transposition of paternally inherited TE families, with gonadal atrophy driven by the death of germline stem cells. Because there are abundant, active TEs in the male inducer genome, that are not present in the female reactive genome, the D. virilis syndrome serves as an excellent model for understanding the effects of hybridization between individuals with asymmetric TE profiles. Results: Using the D. virilis syndrome of hybrid dysgenesis as a model, we sought to determine how the landscape of germline recombination is affected by parental TE asymmetry. Using a genotyping-by-sequencing approach, we generated a high-resolution genetic map of D. virilis and show that recombination rate and TE density are negatively correlated in this species. We then contrast recombination events in the germline of dysgenic versus non-dysgenic F1 females to show that the landscape of meiotic recombination is hardly perturbed during hybrid dysgenesis. In contrast, hybrid dysgenesis in the female germline increases transmission of chromosomes with mitotic recombination. Using a de novo PacBio assembly of the D. virilis inducer genome we show that clusters of mitotic recombination events in dysgenic females are associated with genomic regions with transposons implicated in hybrid dysgenesis. Conclusions: Overall, we conclude that increased mitotic recombination is likely the result of early TE activation in dysgenic progeny, but a stable landscape of meiotic recombination indicates that either transposition is ameliorated in the adult female germline or that regulation of meiotic recombination is robust to ongoing transposition. These results indicate that the effects of parental TE asymmetry on recombination are likely sensitive to the timing of transposition.

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Specific Activation of an I-Like Element in Drosophila Interspecific Hybrids

Cited by 16 publications

References 58 publications

Identification of misexpressed genetic elements in hybrids between Drosophila-related species

Identification of misexpressed genetic elements in hybrids between Drosophila-related species

High‐throughput sequencing of transposable element insertions suggests adaptive evolution of the invasive Asian tiger mosquito towards temperate environments

Hybrid dysgenesis in Drosophila virilis results in clusters of mitotic recombination and loss-of-heterozygosity but leaves meiotic recombination unaltered

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