Specific Adsorption via Peptide Tags: Oriented Grafting and Release of Growth Factors for Tissue Engineering

Abstract

Numerous strategies have been proposed to decorate biomaterials with growth factors (GFs) for tissue engineering applications; their practicability as clinical tools, however, remains uncertain. We previously presented two complementary amphipathic peptides, namely, E5 and K5, which could be utilized as tags to direct GF capture onto organic materials via E5/K5 coiled-coil interactions. We here investigated their potential as mediators of GF physical adsorption. Enzyme-linked immunosorbent assays highlighted that both electrostatic and hydrophobic interactions could contribute to the adsorption process, without interfering with the peptides propensity for coiled-coil interactions. E5-tagged vascular endothelial growth factor, in particular, was efficiently adsorbed to poly(allylamine)-functionalized polystyrene, was maintained in a bioactive state and was steadily liberated over several days with little initial burst. This simple immobilization procedure was successfully applied to poly(ethylene terephthalate) films. Altogether, our data demonstrated that coil-tag-directed adsorption is a tunable, versatile and straightforward strategy to decorate biomaterials with GFs.

Positive role of calcium phosphate ceramics regulated inflammation in the osteogenic differentiation of mesenchymal stem cells

“…IGF was also suggested to play a critical role in tissue regeneration (Urbanek et al, ; Welch et al, ). In this study, protein amount of majority growth factors in Ca‐P ceramic groups was less than that in the control, this may be ascribed to the strong adsorption of porous Ca‐P ceramics for growth factors according to our previous research and other related reports (Chen, Wang, Zhu, Fan, & Zhang, ; Murschel, Zaimi, Noel, Jolicoeur, & Crescenzo, ). However, the high contents of IGF and TGF‐β may make more contribution to subsequent biological events.…”

Growth Factor Engineering for Biomaterials

“…Boucher et al 36 used a coiled-coil interaction to prepare tethered EGF, which elicited enhanced growth of human corneal epithelial cells compared with physically adsorbed or soluble EGF. Murschel et al, 92 who utilized tags to directly capture GFs on organic substrates via coiled-coil interactions, efficiently adsorbed a tagged form of VEGF by poly-(allylamine)-functionalized polystyrene or poly(ethylene terephthalate) film through electrostatic and hydrophobic interactions. The adsorbed VEGF maintained its biological activity and was gradually released over several days.…”

Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine

“…The results revealed specific adsorption of VEGF tagged via amphiphilic peptides through coiled‐coil interactions. The method was able to maintain the bioactive state of the VEGF and was steadily liberated over several days with little initial burst, highlighting the improved adsorption mediated by electrostatic and hydrophobic forces toward the organic substrate . Commonly‐used ECM mimetic proteins/biomolecules for surface functionalization of nanobiomaterials to modulate the stem cell microenvironment in vitro for various tissue engineering applications include: poly‐ d ‐lysine (neurons, stem cells), laminin (neurons, stem cells), fibronectin (endothelium), vitronectin (muscle) gelatin (cardiac), Matrigel (cardiac, epithelial, and stem cells), and collagen (bone and cartilage), to list a few …”