Specific and non-specific interactions in bacterial adhesion to solid substrata

Busscher, Henk J.

doi:10.1016/0378-1097(87)90062-0

Cited by 43 publications

(51 citation statements)

References 29 publications

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“…The three-step model proposed by Busscher and Weerkamp [14] involves Lifshitz-van der Waals forces operating over several hundred nanometers (nm) as a first step. The second operates over distances of about 20 nm involving Lifshitz-van der Waals forces and electrostatic interactions.…”

Section: Attachmentmentioning

confidence: 99%

Bacterial cell attachment, the beginning of a biofilm

Palmer

Flint

Brooks

2007

J Ind Microbiol Biotechnol

598

333

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The ability of bacteria to attach to surfaces and develop into a biofilm has been of considerable interest to many groups in numerous industries, including the medical and food industry. However, little is understood in the critical initial step seen in all biofilm development, the initial bacterial cell attachment to a surface. This initial attachment is critical for the formation of a bacterial biofilm, as all other cells within a biofilm structure rely on the interaction between surface and bacterial cell for their survival. This review examines what are believed to be some of the most important aspects involved in bacterial attachment to a surface.

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Section: Attachmentmentioning

confidence: 99%

Bacterial cell attachment, the beginning of a biofilm

Palmer

Flint

Brooks

2007

J Ind Microbiol Biotechnol

598

333

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“…Most of the attachment between bacteria and proteins are specific ligand-and receptor-like interactions [33,34]. Nonspecific adhesion results from Van der Waals forces acting between the bacteria and the solid substrate and does not involve specific adhesins or ligands [3]. Colonization of bacteria on cellulose is partially due to nonspecific interactions [32].…”

Section: Introductionmentioning

confidence: 99%

Environmental Factors Affecting the Adhesion of Probiotics to Rice Fiber Fractions

et al. 2011

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The adhesion of pure cultures of probiotics to insoluble dietary fiber (IDF1 and IDF2), soluble dietary fiber (SDF1 and SDF2), and total dietary fiber (TDF1 and TDF2) of two rice varieties (RR1 and RR2) was investigated. Bifidobacteria and Lactobacilli adhered to dietary fiber fractions within 15 to 30 min. A higher percentage of Bifidobacteria (40-70%) adhered to rice fiber than lactobacilli (<40%). Adhesion was not affected by NaCl, or Tween 80 but, was reduced by various sugars and polysaccharides (maltose, moltodextrin, amylopectin, and amylose). The optimal temperature for adhesion was 37°C and live cells are important for adhesion. Adhesion was inhibited by protease and by low pH (pH 3-4). Adhesion was not influenced by proteins or peptides linked with the fiber but involved bacterial cell surface proteins or glycoproteins.

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“…Other studies found linear colonization patterns with decreasing or increasing surface tension [1,28,49]. Exopolymeric compounds are the main mediators in adhesion of bacteria and diatoms to surfaces [9,13,14,16,18,27,33,51,55,58]. The bulk of bacterial exopolymers consists of polysaccharides.…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharide production and attachment strength of bacteria and diatoms on substrates with different surface tensions

Becker¹

1996

Microb Ecol

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Attachment strength and exopolysaccharide (EPS) production of Pseudomonas sp. (bacteria) and the diatom Amphora coffaeformis were studied on six different substrata with surface tensions between 19 and 64.5 mN m -1. Test panels of the materials were exposed to bacterial cultures between 3 and 120 hours, and to diatom cultures between 48 and 72 hours. Exopolysaccharide production by surface-associated cells was measured using the phenol sulfuric acid method. Attachment studies were run by exposing test panels to laminar flow pressure using a radial flow chamber. Highest EPS production by bacteria and diatoms was recorded on substrata with surface tensions above 30 mN m -1. Lowest EPS production occurred on substrata between 20 and 25 mN m -1. Highest EPS production and strongest adhesion was found on polycarbonate (33.5 mN m-~). Both test organisms improved their attachment strength with exposure time on most materials. However, amounts of produced EPS and improvement of attachment indicated that mechanisms other than polysaccharide production are more important on substrata with low surface tensions (<25 mN m-l). Simply producing more polysaccharides is not sufficient to overcome weak attachment on materials with low surface tensions. For example, adhesion of Pseudomonas sp. and A. coffaeformis on polytetrafluorethylene/perfluor-copolymer (PFA; 22 mN m -1) and glass (64.5 mN m ~) was equally strong although EPS production was much higher on glass than on PFA. This is somewhat surprising for A. coffaeformis because polysaccharide production has been considered the most important attachment mechanism of A. coffaeformis.

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Specific and non-specific interactions in bacterial adhesion to solid substrata

Cited by 43 publications

References 29 publications

Bacterial cell attachment, the beginning of a biofilm

Bacterial cell attachment, the beginning of a biofilm

Environmental Factors Affecting the Adhesion of Probiotics to Rice Fiber Fractions

Exopolysaccharide production and attachment strength of bacteria and diatoms on substrates with different surface tensions

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