SPECIFIC AND SENSITIVE DETERMINATION OF PREGNANCY-SPECIFIC Β1-Glycoprotein BY RADIOIMMUNOASSAY

Grudzinskas, J. G.; Jeffrey, Diana; Gordon, Y. B.; Chard, Tim

doi:10.1016/s0140-6736(77)91136-9

Cited by 110 publications

(27 citation statements)

References 7 publications

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“…SP, readioimn maunoassay. Estinmations of SP, were performed using a polyethylene glycol precipitation radioimmunoassay described elsewhere (Grudzinskas et al, 1977). The minimuim detection limit was 20 ,tg/l.…”

mentioning

confidence: 99%

Serum levels of human placental lactogen and pregnancy-specific β1-glycoprotein in breast cancer

Monteiro¹,

Biswas²,

Al-Awqati³

et al. 1982

Br J Cancer

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mentioning

confidence: 99%

Serum levels of human placental lactogen and pregnancy-specific β1-glycoprotein in breast cancer

Monteiro¹,

Biswas²,

Al-Awqati³

et al. 1982

Br J Cancer

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“…1. Radioimmunoassay.-Essentially the same technique was used as for the radioimmunometric assay, except that a 20% solution of polyethylene glycol 6000 was substituted for the second antibody (Grudzinskas et al, 1977). After standing at 4°C for 72 h and the assay.…”

Section: Methodsmentioning

confidence: 99%

An improved antigenic marker of human lung carcinomas and its use in radioimmunoassays

Wolf¹,

Micksche²,

Bauer³

1981

Br J Cancer

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Summary.-An antigenic activity in pleural effusions of patients with squamous-cell carcinoma of the lung has been prepared in highly purified form by a 5-step fractionation scheme. The purified substance, designated LuCA (lung cancer antigen), was assessed during the course of the fractionation procedure by a radioimmunometric assay carried out with specific soluble reagents. Sensitive saturation-binding assays showed no or only weak uptake of the 1251-labelled antigen preparation by a panel of antisera specific for known bronchogenic tumour markers, and for normal human serum proteins. The preparation appeared to contain lung-tumour-associated antigens, one of them probably distinct for squamous-cell carcinomas. The antigen fraction consists of acid-soluble glycoproteins, and was demonstrated by SDSpolyacrylamide gel electrophoresis as a single band in the mol. wt region of 43,000. The gel-filtration elution volume appeared to indicate the occurrence of the antigenic activity in multiples of this smaller unit. Pilot radioimmunoassays performed with LuCA and an absorbed specific antiserum suggest the possible suitability of the marker preparation for screening lung-cancer patients.

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“…The evidence concerning the presence of S?i in the serum of healthy men and non-pregnant women is conflicting (6)(7)(8)(9).…”

Section: Levels Increase Rapidlymentioning

confidence: 99%

Evaluation and Modification of a Radioimmunoassay for Pregnancy-specific β₁-Glycoprotein

Bonte¹,

Veer²

1981

CCLM

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Summary: The radioimmunoassay available from Behringwerke for pregnancy-specific ft-glycoprotein (S?i) was tested for its ability to detect pregnancy prior to the first missed menstrual period. It was found that the equine serum, used as solvent for the standards, did not react like human serum.The standard solvent was replaced by bovine serum albumin 50 g/1 and pooled human serum respectively. Equilibrium and sequential incubation procedures were compared. The latter appeared to be more sensitive in the low value range and was therefore more suitable for the early detection of pregnancy.Also, with standards in albumin, the sequential assay was more specific.SPj could be detected in sera of men and non-pregnant women, using albumin as standard solvent. This could be due to different cross reacting material of the protein matrix, or to the presence of SP^like material in human sera.The choice of human male serum seemed to be the most practical. It has also been adopted by Behring, and a commercial kit has been prepared. Prüfung, Bewertung und Modifikation eines Radioimmunassay fur schwangerschaftsspezifisches ß^GlykoproteinZusammenfassung: Der bei den Behringwerken erhältliche Radioimmunassay für schwangerschaftsspezifisches ft-Glykoprotein wurde auf seine Eignung, eine Schwangerschaft bereits vor der ersten ausgebliebenen Menstrualblutung nachzuweisen, geprüft.

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SPECIFIC AND SENSITIVE DETERMINATION OF PREGNANCY-SPECIFIC Β1-Glycoprotein BY RADIOIMMUNOASSAY

Cited by 110 publications

References 7 publications

Serum levels of human placental lactogen and pregnancy-specific β1-glycoprotein in breast cancer

Serum levels of human placental lactogen and pregnancy-specific β1-glycoprotein in breast cancer

An improved antigenic marker of human lung carcinomas and its use in radioimmunoassays

Evaluation and Modification of a Radioimmunoassay for Pregnancy-specific β₁-Glycoprotein

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SPECIFIC AND SENSITIVE DETERMINATION OF PREGNANCY-SPECIFIC Β1-Glycoprotein BY RADIOIMMUNOASSAY

Cited by 110 publications

References 7 publications

Serum levels of human placental lactogen and pregnancy-specific β1-glycoprotein in breast cancer

Serum levels of human placental lactogen and pregnancy-specific β1-glycoprotein in breast cancer

An improved antigenic marker of human lung carcinomas and its use in radioimmunoassays

Evaluation and Modification of a Radioimmunoassay for Pregnancy-specific β1-Glycoprotein

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Product

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Evaluation and Modification of a Radioimmunoassay for Pregnancy-specific β₁-Glycoprotein