Specific Antibody Levels in the Intestinal Perfusates of &lt;i&gt;Heligosomoides polygyrus&lt;/i&gt;-lnfected Mice

Cypess, Raymond H.; Ebersole, J. L.; Molinari, John A.

doi:10.1159/000231963

Cited by 11 publications

(1 citation statement)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Only IgA antibodies were measured at significant levels in the intestinal lumen of mice 28 days after infection with E. caproni, whereas no IgM or IgG antibodies could be detected. The predominance of the IgA response in the intestinal lumen is consistent with other studies on immune responses to intestinal helminth infections (POULAIN et al, 1976;CYPESS et al, 1977;BEFUS, 1977;VAN DER VORST et al, 1989), and with the secretory nature of this antibody class (BEFUS & BIENENSTOCK, 1982;KAWANISHI, 1989). When secreted into the gut lumen, IgA is complexed to a secretory component, which protects it from proteolytic degradation.…”

Section: Discussionsupporting

confidence: 89%

The antibody response in serum, intestinal wall and intestinal lumen of NMRI mice infected with Echinostoma caproni

Agger¹,

Simonsen²,

Vennervald³

1993

J. Helminthol.

View full text Add to dashboard Cite

Groups of mice (NMRI) were infected with 0.6 and 25 metacercariae of the intestinal trematode Echinostoma caproni. An enzyme linked immunosorbent assay (ELISA) was developed for measuring the specific IgM, IgG and IgA antibody responses in serum, small intestinal tissue and small intestinal lumen of the mice, by using a crude adult E. caproni antigen. In infected mice, significant levels of IgM were measured in the sera from day 14 after infection and of IgG and IgA from day 28 after infection. Early in the infection, the mean level of serum IgM was higher in 6-worm infections than in 25-worm infections. Late in the infection, higher mean levels of IgA were reached in the serum of mice with high than in those with low dose infections. The onset of appearance of antibodies in the tissue of the small intestine reflected the picture seen in the serum. For IgM, and to a lesser degree for IgG, the highest mean antibody levels appeared in the posterior sections of the small intestinal wall, where also the parasites were located. The mean level of IgA, however, was uniform throughout the length of the small intestinal tissue. High levels of specific IgA were detected in the lumen of the small intestine on day 28 after infection, especially in the anterior sections, where only few parasites were located. No specific IgM or IgG could be detected in the intestinal lumen on this day. The results are related to the intestinal location and the pattern of expulsion of E. caproni in the mouse host.

show abstract

Section: Discussionsupporting

confidence: 89%

The antibody response in serum, intestinal wall and intestinal lumen of NMRI mice infected with Echinostoma caproni

Agger¹,

Simonsen²,

Vennervald³

1993

J. Helminthol.

View full text Add to dashboard Cite

show abstract

Irradiated larval vaccination and antibody responses evaluated in relation to the expression of immunity to Heligmosomoides polygyrus

Pleass

Bianco

1996

Parasitol Res

View full text Add to dashboard Cite

Infections induced in NIH mice by irradiated (300 Gy) larvae of Heligmosomoides polygyrus effectively stimulated immunity to challenge, whereas unirradiated larvae did not. Importantly, this difference was lost by the elimination of the adult worms arising from unirradiated sensitising infections by drug treatment prior to challenge. No difference in the level of parasite-specific serum and mucosal IgG, IgG1, IgG2a, or IgA was detected between immune mice sensitised either with drug-abbreviated unirradiated or irradiated larval infections and non-immune mice receiving two superimposed unirradiated infections. An enzyme-linked immunosorbent assay (ELISA) and immunoblotting data suggested that parasite-specific IgG1 was the predominant antibody class in both serum and intestinal perfusates. IgA exhibited differences in antigen specificity between the serum and the intestine. In serum, IgA responses were directed predominantly to L4 somatic antigens, whereas at the mucosal surface they were biased towards L4 excretory/secretory (ES) antigens. No correlation was found between the intensity of the serum or mucosal antibody responses and the mean worm burdens in groups of immune or non-immune mice. Moreover, no correlation was found between levels of parasite-specific serum or mucosal IgG, IgG1, IgG2a or IgA and the loss of worms in individual mice.

show abstract

Hymenolepis microstoma: Mouse strain differences in resistance to a challenge infection

Murray

Foster

Passmore

1984

Experimental Parasitology

View full text Add to dashboard Cite

Specific Antibody Levels in the Intestinal Perfusates of <i>Heligosomoides polygyrus</i>-lnfected Mice

Cited by 11 publications

References 7 publications

The antibody response in serum, intestinal wall and intestinal lumen of NMRI mice infected with Echinostoma caproni

The antibody response in serum, intestinal wall and intestinal lumen of NMRI mice infected with Echinostoma caproni

Irradiated larval vaccination and antibody responses evaluated in relation to the expression of immunity to Heligmosomoides polygyrus

Hymenolepis microstoma: Mouse strain differences in resistance to a challenge infection

Contact Info

Product

Resources

About