Specific binding of prostaglandin F2α to membranes of rat corpora lutea

Wright, K.; Luborsky-Moore, J.L.; Behrman, Harold R.

doi:10.1016/0303-7207(79)90073-x

Cited by 36 publications

(8 citation statements)

References 10 publications

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“…First, using our experimental conditions, we were able to measure PGF-2a binding in a membrane preparation from the ovaries of rats in late dioestrus or pro-oestrus. The PGF-2a binding level, affinity and specificity were similar to those described by others (Wright et al, 1979;Müller et al, 1981) in the same experimental model. Secondly, we successfully detected high-affinity PGE-2 binding sites in the same endometrial preparations that did not exhibit high affinity PGF-2a binding.…”

Section: Discussionsupporting

confidence: 79%

“…The receptor concentration (1000 fmol/mg protein) as well as the receptor specificity Text- fig. 4) were similar to those previously described for this receptor (Rao, 1976;Wright et al, 1979;Müller, Bauknecht & Siebers, 1981).…”

Section: Methodsmentioning

confidence: 65%

“…Membrane suspensions were prepared as described by Wright, Luborsky-Moore & Behrman (1979). In summary, the homogenate was centrifuged at 700 # for 10 min, and the 700 g supernatant was then centrifuged at 10 000 £ for 45 min.…”

Section: Methodsmentioning

confidence: 99%

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Failure to detect specific binding sites for prostaglandin F-2 in membrane preparations from rat endometrium

Martel¹,

Kennedy²,

Monier³

et al. 1985

Reproduction

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Membrane preparations from endometria of rats in different physiological states (e.g. pseudopregnancy, ovariectomized animals receiving progesterone + oestradiol or oestradiol alone) were studied for [3H]PGF-2 alpha binding by methods which detected PGF-2 alpha binding in ovary preparations and PGE binding in the same endometrial preparations. There was no evidence of high-affinity binding sites for [3H]PGF-2 alpha. Saturable [3H]PGF-2 alpha binding that increased with the onset of uterine sensitivity was detected but this binding does not fulfil all the criteria required for a PGF-2 alpha receptor and is probably due to binding to PG metabolizing enzymes in our preparations, or to binding of [3H]PGF-2 alpha to PGE binding sites. The failure to detect specific PGF-2 alpha binding sites seems to reflect a true absence of these sites in the rat endometrium.

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Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 65%

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Failure to detect specific binding sites for prostaglandin F-2 in membrane preparations from rat endometrium

Martel¹,

Kennedy²,

Monier³

et al. 1985

Reproduction

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“…Administration of PGF 2 induces abortion (Deis 1971), and induction of 20 -hydroxysteroid dehydrogenase (20 -HSD) activity (Pharriss & Wyngarden 1969, Strauss & Stambaugh 1974, Lamprecht et al 1975, Bussmann & Deis 1979, an enzyme that converts P 4 into a derivative devoid of progestational activity and considered a marker of luteolysis (Wiest 1968. The role of PGF 2 as an inducer of the physiological luteolysis in the rat is corroborated by the demonstration of specific receptors for PGF 2 on luteal membranes (Wright et al 1979, Bussmann 1989). Locally synthesised PGs have an important role in CL regression as indicated by an increase in the content of PGF 2 in the CL during spontaneous luteolysis (Olofsson & Selstam 1988).…”

Section: Introductionmentioning

confidence: 95%

Participation of intraluteal progesterone and prostaglandin F2 alpha in LH-induced luteolysis in pregnant rat

Stocco

Deis

1998

Journal of Endocrinology

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We examined the participation of the intraluteal levels of progesterone (P 4 ) and prostaglandin F 2 (PGF 2 ) in the induction of luteolysis by LH and its relationship with the induction of the 20 -hydroxysteroid dehydrogenase activity (20 -HSD). Subcutaneous administration of four doses of 10 µg ovine LH (oLH) at 0800, 0900, 1000 and 1100 h on day 19 of pregnancy induced a decrease in the activity of the enzyme 3 -HSD 24 and 48 h after treatment and an increase in luteal 20 -HSD activity 48 h after oLH treatment when compared with control rats. Intraluteal and serum P 4 levels were lower than control values 24 and 48 h after oLH treatment, with a significant increase in luteal PGF 2 content and a decrease in corpus luteum (CL) weight 48 h after oLH treatment. Intrabursal ovarian (IB) treatment with an inhibitor of PG's biosynthesis (diclofenac) (70 µg/ovary) or P 4 (3 µg/ovary) on day 20 of pregnancy, prevented the increase in 20 -HSD activity observed 48 h after oLH treatment, without any effect on 3 -HSD activity. The IB administration of P 4 prevented the increase in intraluteal PGF 2 content induced by oLH treatment and the increases in 20 -HSD activity and intraluteal PGF 2 content observed in control animals on day 21 of pregnancy. The inhibition of PG biosynthesis also prevents the decrease in intraluteal and serum P 4 level induced by oLH. These results provide good evidence of the important participation of intraluteal P 4 and PGF 2 on the oLH-induced luteolysis in pregnant rats. We also found that P 4 produced by the CL is involved, in part, in the regulation of luteal PG synthesis. Thus, the early decline in 3 -HSD activity and the consequent fall in intraluteal P 4 content, may trigger the synthesis of PGs and thereafter the increase in luteal 20 -HSD activity to establish luteolysis.

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“…Therefore the apparent cyclic variation of prostaglandin receptors must be ascribed to occupancy of the receptors by varying amounts of endogenous prostaglandin F2\g=a\. Specific receptors for prostaglandin Faa (PGF2a) are found in the ovaries of various species includ¬ ing human (Powell et al 1974;Rao et al 1977;Schillinger et al 1979), bovine (Rao 1974(Rao , 1975Kimball & Lauderdale 1975) and rat (Wright et al 1979;Schillinger et al 1979). The oscillation of receptors disappears after inhibition of prostaglandin synthesis by indomethacin.…”

mentioning

confidence: 99%

Development and regulation of the prostaglandin F2α receptor in the rat ovary

Müller¹,

Bauknecht²,

Siebers³

1981

Acta Endocrinologica

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Ovaries of adult rats specifically bind PGF2\g=a\ while those of immature animals do not. Induction of luteinization by hCG in juvenile animals, however, results in specific binding of PGF2\g=a\ It is suggested that luteal cells are the only cell type of the ovary, which is endowed with specific receptors for PGF2\g=a\The number of PGF2\g=a\ binding sites varies during the ovarian cycle. Most free

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Specific binding of prostaglandin F2α to membranes of rat corpora lutea

Cited by 36 publications

References 10 publications

Failure to detect specific binding sites for prostaglandin F-2 in membrane preparations from rat endometrium

Failure to detect specific binding sites for prostaglandin F-2 in membrane preparations from rat endometrium

Participation of intraluteal progesterone and prostaglandin F2 alpha in LH-induced luteolysis in pregnant rat

Development and regulation of the prostaglandin F2α receptor in the rat ovary

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