Specific biological agent taggants

Kiel, Johnathan L.; Holwitt, Eric A.; Parker, Jill E.; Vivekananda, Jeeva; Franz, Veronica; Sloan, Mark A.; Miziolek, Andrzej W.; DeLucia, Frank C.; Munson, Chase A.; Mattley, Yvette

doi:10.1117/12.597640

Cited by 6 publications

(6 citation statements)

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“…Such emission features from the remnants of organic molecules and also from calcium and phosphorus are fairly ubiquitous in the LIBS spectra acquired from biological specimens. To overcome this, Kiel et al showed that by restricting analysis only to those metals endogenously present and added by preferentially "tagging" cells using custom specific binding agents tagged with exotic metals such as scandium and europium, the identification of Bacillus spores could be performed with a high degree of confidence [44]. Figure 1.…”

Section: Initial Studiesmentioning

confidence: 99%

“…In some of the early experiments previously described, it was initially believed that a univariate analysis of the intensities or ratios of intensities of certain lines in the LIBS spectrum (e.g. Ca or Mg) could provide sufficient information to discriminate the bacteria [28,39,40,43,44]. Other groups utilized modified linear correlation techniques [42] comparing the relative intensities of one or several elements [48].…”

Section: Chemometricsmentioning

confidence: 99%

“…More recently, we have utilized a highly convenient microbiological filter medium, which provides a highly stable, reproducible, but non-background-free (especially from carbon) mounting substrate [70]. Such filters, fabricated from nitrocellulose paper, nylon, or other material, have been used extensively by other groups due to their ubiquity and availability [44,47,49,75].…”

Section: Aerosolsmentioning

confidence: 99%

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A review of the use of laser-induced breakdown spectroscopy for bacterial classification, quantification, and identification

Rehse

2019

Spectrochimica Acta Part B: Atomic Spectroscopy

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The use of laser-induced breakdown spectroscopy to determine the elemental composition of bacterial cells has been described in the peer-reviewed literature since 2003. Fifteen years on, significant accomplishments have been reported that have served to clarify and underscore the areas of bacteriological investigation that LIBS is well-suited for as well as the challenges that yet remain to be faced. This review will attempt to summarize the state of the field by surveying the available body of knowledge. The early days of these experiments, roughly from 2003 to 2007, in which many of the most fundamental experiments were initially conducted will be described. The more in-depth investigations that followed in the subsequent decade will then be detailed. Many important aspects of performing LIBS on bacterial cells were reported on and are summarized here including: the use of chemometric algorithms for statistical classification of unknown spectra; the influence of the mounting substrate on classification; the effect of the testing gas atmosphere and the choice of bacterial cell growth nutrient medium on the measured LIBS spectrum; the efficacy of a LIBS-based test as a genus-level or strainlevel discrimination test; the ability of LIBS to determine the cell titer or concentration of cells in the initial sample; the effects that possible contaminations or interferents within the sample would have on the LIBS spectrum; the influence that environmental stresses the cells may be exposed to during growth and the state of reproductive health of the cells could have on the LIBS spectrum; the use of standoff or remote apparatus to minimize the risk to the operators during bacteriological identification of unknown specimens; and the combination of other optical modalities with LIBS to enhance the sensitivity or specificity of identification. Lastly, tables are provided which summarize both every species of bacteria ever tested with LIBS as well as the major lessons learned by the community through 15 years of careful investigation. laser-induced breakdown spectroscopy; pathogens; bacteria

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Section: Initial Studiesmentioning

confidence: 99%

Section: Chemometricsmentioning

confidence: 99%

Section: Aerosolsmentioning

confidence: 99%

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A review of the use of laser-induced breakdown spectroscopy for bacterial classification, quantification, and identification

Rehse

2019

Spectrochimica Acta Part B: Atomic Spectroscopy

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“…In a similar manner, Boyain-Goitia et al, 2003 andTelle 2005 reported the work done employing LIBS on different kinds of bioaerosols. In the beginning of 2003, and motivated by the Bacillus anthracis Banthrax^bioterrorism attacks, various breakthrough LIBS experiments were performed to indicate its potential usefulness for rapid detection of injurious Gram-negative and Gram-positive bacteria and spores (Samuels et al 2003;Morel et al, 2003;Hybl et al 2003;Kim et al 2004;Kiel et al 2005;Leone et al 2004;Delucia et al 2005).…”

Section: Analysis Of Microorganisms Causing Human Diseasementioning

confidence: 99%

Laser-induced breakdown spectroscopy (LIBS): a novel technology for identifying microbes causing infectious diseases

et al. 2018

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With the advent of improved experimental techniques and enhanced precision, laser-induced breakdown spectroscopy (LIBS) offers a robust tool for probing the chemical constituents of samples of interest in biological sciences. As the interest continues to grow rapidly, the domain of study encompasses a variety of applications vis-à-vis biological species and microbes. LIBS is basically an atomic emission spectroscopy of plasma produced by the high-power pulsed laser which is tightly focused on the surface of any kinds of target materials in any phase. Due to its experimental simplicity, and versatility, LIBS has achieved its high degree of interest particularly in the fields of agricultural science, environmental science, medical science, forensic sciences, and biology. It has become a strong and sensitive elemental analysis tool as compared to the traditional gold standard techniques. As such, it offers a handy, rapid, and flexible elemental measurement of the sample compositions, together with the added benefits of less cumbersome sample preparation requirements. This technique has extensively been used to detect various microorganisms, extending the horizon from bacteria, molds, to yeasts, and spores on surfaces, while also being successful in sensing diseasecausing viruses. LIBS-based probe has also enabled successful detection of bacteria in agriculture as well. In order for good quality processing of food, LIBS is also being used to detect and identify bacteria such as Salmonella enteric serovar typhimurium that causes food contamination. Differences in soil bacteria isolated from different mining sites are a very good indicator of relative environmental soil quality. In this connection, LIBS has effectively been employed to discriminate both the inter-and intra-site differences of the soil quality across varying mining sites. Therefore, this article summarizes the basic theory and use of LIBS for identifying microbes causing serious agricultural and environmental infectious diseases.

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“…A superior choice to all of these is the use of inexpensive, disposable filter media. Other groups have made extensive use of such filters, which are fabricated from nitrocellulose paper, nylon, or other material, due to their widespread availability and their familiarity to clinical microbiologists [7,26,27,28,29]. The ability to quickly separate bacterial cells in a liquid suspension from the liquid itself using the micropores of the filter in their intended role is an obvious advantage.…”

Section: Introductionmentioning

confidence: 99%

A simple and efficient centrifugation filtration method for bacterial concentration and isolation prior to testing liquid specimens with laser-induced breakdown spectroscopy

Malenfant

Paulick

Rehse

2019

Spectrochimica Acta Part B: Atomic Spectroscopy

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Specific biological agent taggants

Cited by 6 publications

References 0 publications

A review of the use of laser-induced breakdown spectroscopy for bacterial classification, quantification, and identification

A review of the use of laser-induced breakdown spectroscopy for bacterial classification, quantification, and identification

Laser-induced breakdown spectroscopy (LIBS): a novel technology for identifying microbes causing infectious diseases

A simple and efficient centrifugation filtration method for bacterial concentration and isolation prior to testing liquid specimens with laser-induced breakdown spectroscopy

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