Specific Capture and Release of Circulating Tumor Cells Using Aptamer‐Modified Nanosubstrates

Shen, Qinglin; Xu, Li; Zhao, Libo; Wu, Danni; Fan, Yunshan; Zhou, Yiliang; OuYang, Wei‐Han; Xu, Xiaochun; Zhang, Zhen; Song, Min; Lee, Tom; Garcia, Mitch A.; Xiong, Bin; Hou, Shuang; Tseng, Hsian‐Rong; Fang, Xiaohong

doi:10.1002/adma.201300082

Cited by 283 publications

(268 citation statements)

References 39 publications

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“…Während der letzten Jahre wurde eine Reihe von Systemen entwickelt, die die effiziente Gewinnung von Tu morzellen nach der Einfangreaktion erlauben. Die Ablçsung erfolgt über chemische [65] und enzymatische Mechanismen, [66,67] über Selbstorganisation, [68] mechanosensitiv [69] oder thermisch [70,71] (Abbildung 4). Dabei wurde ein hoher Grad an Lebensfä-higkeit der Tu morzellen bei gleichzeitig geringer Kontamination durch andere Zellen erreicht.…”

Section: Markerfreie Isolierung Von Ctcsunclassified

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Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Green

Safaei²,

Mepham

et al. 2015

Angewandte Chemie

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In den letzten zehn Jahren gab es wichtige Fortschritte bei der Erfassung seltener zirkulierender Tumorzellen (CTCs) aus dem Blut von Krebspatienten als eine entscheidende Voraussetzung für die nichtinvasive Tumorprofilierung. Diese Fortschritte haben zudem neue Einblicke in die Materialchemie und die Mikrofluidik erbracht und ermöglichten die Erfassung und Auszählung von CTCs mit beispielloser Empfindlichkeit. Allerdings wurde auch immer klarer, dass einfaches Isolieren und Auszählen von Tumorzellen, die in den Kreislauf gelangt waren, nicht die benötigten Informationen lieferte, um unser Verständnis der Biologie dieser seltenen Zellen zu vertiefen oder um sie sich in der Therapie besser zunutze zu machen. Mithilfe von Apparaturen und Materialien der nächsten Generation mit maßgeschneiderten physikalischen und chemischen Eigenschaften ist man inzwischen in der Lage, mehr Informationen aus CTCs zu gewinnen. In diesem Kurzaufsatz werden die Arbeiten der letzten zehn Jahre in diesem Gebiet diskutiert, wobei insbesondere die bahnbrechenden Studien der letzten fünf Jahre Berücksichtigung finden, die den Blick über das einfache Isolieren von CTCs hinaus auf Ansätze gerichtet haben, die eine tiefergehende Analyse ermöglichen.

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Section: Markerfreie Isolierung Von Ctcsunclassified

“…Aptamere,d ie in großen DNA-Netzwerken [66] oder auf Silicium-Nanodrähten (SiNWs) [67] immobilisiert sind, werden zum Einfangen von Zellen verwendet. Die Freisetzung der Zellen gelingt durch Nukleasebehandlung.M it aptamermodifizierten Nanodrähten gelang eine Einfangrate von 95 % und eine Gewinnung von 94 %b ei Lungenkrebszellen.…”

Section: Abfangen Und Freisetzen Von Ctcsunclassified

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Green

Safaei²,

Mepham

et al. 2015

Angewandte Chemie

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“…CTCs provide an opportunity to noninvasively monitor cancer patients' prognosis and response to therapy. Over the past few decades, although great endeavor has been devoted to developing CTC capture and recovery devices in which captured cells are released usually under certain special stimuli (such as light, electric current, enzymes, temperature, and pH), [7][8][9][10][11] these techniques are often limited by a capture and recovery performance tradeoff between high efficiency and high viability. Hence, there is a desperate need for techniques to capture and recover high viability CTCs.…”

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confidence: 99%

“…We also developed a platform that is capable of not only capturing CTCs with high efficiency but also recovering the CTCs with high purity and viability upon two rounds of nuclease treatment. 8 Although efficient and reproducible enumeration of CTCs has been achieved, the several heating/cooling process cycles or multiple rounds of enzyme treatment may affect the recovery cell morphology and viability; one of the remaining obstacles is to minimize damaged cells and improve cell viability performance.…”

mentioning

confidence: 99%

“…Therefore, based on our previous studies, 7,8 we anticipated that further improvement of cell viability performance could be achieved by decreasing the heating/cooling cycles and enzymatic treatment rounds. Thermoresponsive poly (N-isopropylacrylamide) (PNIPAM) [15][16][17][18][19][20] is one of typical thermosensitive polymers with a good biocompatible and a lower critical solution temperature behavior approximately 32°C in an aqueous solution, which is close to the physiological temperature, so PNIPAM is widely applied to the bioseparation.…”

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confidence: 99%

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Aptamer-polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing

Shen

Peng

Zhan

et al. 2016

IJN

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Selection of the optimal chemotherapy regimen for an individual cancer patient is challenging. The existing chemosensitivity tests are costly, time-consuming, and not amenable to wide utilization within a clinic. This limitation might be addressed by the recently proposed use of circulating tumor cells (CTCs), which provide an opportunity to noninvasively monitor response to therapy. Over the past few decades, various techniques were developed to capture and recover CTCs, but these techniques were often limited by a capture and recovery performance tradeoff between high viability and high efficiency. In this work, we used anti-epithelial cell adhesion molecule coated aptamer–poly (N-isopropylacrylamide) functionalized silicon nanowire substrates to capture and release epithelial cell adhesion molecule-positive CTCs at 32°C and 4°C, respectively. Then, we applied the nuclease to digest the aptamer to release the captured CTCs (near or at the end of the polymer brush), which cannot be released by heating/cooling process. High viability and purity CTCs could be achieved by decreasing the heating/cooling cycles and enzymatic treatment rounds. Furthermore, the time-saving process is helpful to maintain the morphology and enhance vitality of the recovered CTCs and is beneficial to the subsequent cell culture in vitro. We validated the feasibility of chemosensitivity testing based on the recovered HCC827 cells using an adenosine triphosphate–tumor chemosensitivity assay, and the results suggested that our method can determine which agent and what concentration have the best chemosensitivity for the culturing recovered CTCs. So, the novel method capable of a highly effective capture and recovery of high viability CTCs will pave the way for chemosensitivity testing.

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Nanovelcro Cell‐Affinity Assay for Detecting and Characterizing Circulating Tumor Cells

Lin

Fong

Chen

et al. 2016

Circulating Tumor Cells

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Specific Capture and Release of Circulating Tumor Cells Using Aptamer‐Modified Nanosubstrates

Cited by 283 publications

References 39 publications

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Profilierung zirkulierender Tumorzellen mit Apparaturen und Materialien der nächsten Generation

Aptamer-polymer functionalized silicon nanosubstrates for enhanced recovered circulating tumor cell viability and in vitro chemosensitivity testing

Nanovelcro Cell‐Affinity Assay for Detecting and Characterizing Circulating Tumor Cells

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