Specific Characteristics of an Endoglucanase RCE1 from Rhizopus Oryzae in the Treatment of the Dyed Cotton Fabrics

Nakane, Akitaka; Koga, Jinichiro; Kubota, Hitomi; Kono, Toshiaki

doi:10.2115/fiber.61.229

Cited by 5 publications

(2 citation statements)

References 9 publications

(18 reference statements)

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“…Based on the results on May 9, 2018 by guest http://aem.asm.org/ described here, STCE1 is considered to be an all-around suitable enzyme that has all the properties required for laundry use. Although the catalytic domain of RCE1 had high defibrillation activities on dyed cotton and lyocell fabrics, this enzyme had low resistance to anionic surfactants and oxidizing agents (29,35). By contrast, the catalytic domain of EGL3 was highly resistant to anionic surfactants and oxidizing agents, whereas it had low defibrillation activity on dyed lyocell fabrics (35).…”

Section: Resultsmentioning

confidence: 98%

“…However, all-purpose enzymes that have all the properties mentioned above have not been obtained yet. For example, although family 45 endoglucanases from members of the Zygomycota, such as RCE1 and RCE2 from Rhizopus oryzae (23,25), MCE1 and MCE2 from Mucor circinelloides (2), and PCE1 from Phycomyces nitens (34), had high defibrillation activities on dyed lyocell and cotton fabrics, they had little resistance to an anionic surfactant and an oxidizing agent (29,35). By contrast, family 45 endoglucanases from members of the Ascomycota, such as EGL3 and EGL4 from Humicola grisea (38), were highly resistant to the anionic surfactant and oxidizing agent, whereas they had low defibrillation activity on the dyed lyocell fabric (35).…”

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Purification and Characterization of a New Family 45 Endoglucanase, STCE1, from Staphylotrichum coccosporum and Its Overproduction in Humicola insolens

Koga

Baba

Shimonaka

et al. 2008

Appl Environ Microbiol

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In the detergent industry, fungal endoglucanases have been used to release microfibrils (defibrillation) from the surface of dyed cellulosic fabrics to enhance color brightness. Although endoglucanases for laundry use must have various properties, such as a neutral or alkaline optimum pH, resistance to anionic surfactants and oxidizing agents (main components in detergents), and high defibrillation activity, all-purpose endoglucanases have not been obtained yet. As a result of screening of endoglucanases, a new family 45 endoglucanase (family 45 glycoside hydrolase), designated STCE1, was obtained and purified to apparent homogeneity from the culture supernatant of Staphylotrichum coccosporum NBRC 31817. The molecular mass of STCE1 was 49 kDa. The optimum pH for the carboxymethyl cellulase activity of STCE1 was 6.0, and the optimum temperature was 60°C. STCE1 was highly resistant to an anionic surfactant and an oxidizing agent. Furthermore, the defibrillation activities on dyed cotton and lyocell fabrics of STCE1 were higher than those of the other representative endoglucanases tested. These results indicate that STCE1 is an all-purpose enzyme for laundry use. A gene encoding STCE1, designated the stce1 gene, was cloned from S. coccosporum, and the complete sequence was determined. STCE1 consisted of three distinct domains: an N-terminal catalytic domain (family 45), a linker domain, and a C-terminal carbohydrate-binding module (family 1). The amino acid sequences of the catalytic domain of STCE1 were phylogenetically close to those of the family 45 endoglucanases EGL3, EGL4, and EGV from a Humicola sp. Hence, the stce1 gene was transferred into Humicola insolens and expressed. As a result, extremely high levels (0.90 mg protein per ml of culture supernatant, 27% of the total proteins) of the recombinant STCE1 were secreted as a mature form in the culture supernatant.

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confidence: 99%

Purification and Characterization of a New Family 45 Endoglucanase, STCE1, from Staphylotrichum coccosporum and Its Overproduction in Humicola insolens

Koga

Baba

Shimonaka

et al. 2008

Appl Environ Microbiol

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Construction of a promoter collection for genes co-expression in filamentous fungus Trichoderma reesei

Wang

Meng

Liu

et al. 2014

Journal of Industrial Microbiology and Biotechnology

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Trichoderma reesei is the preferred organism for producing industrial cellulases. However, cellulases derived from T. reesei have their highest activity at acidic pH. When the pH value increased above 7, the enzyme activities almost disappeared, thereby limiting the application of fungal cellulases under neutral or alkaline conditions. A lot of heterologous alkaline cellulases have been successfully expressed in T. reesei to improve its cellulolytic profile. To our knowledge, there are few reports describing the co-expression of two or more heterologous cellulases in T. reesei. We designed and constructed a promoter collection for gene expression and co-expression in T. reesei. Taking alkaline cellulase as a reporter gene, we assessed our promoters with strengths ranging from 4 to 106 % as compared to the pWEF31 expression vector (Lv D, Wang W, Wei D (2012) Construction of two vectors for gene expression in Trichoderma reesei. Plasmid 67(1):67-71). The promoter collection was used in a proof-of-principle approach to achieve the co-expression of an alkaline endoglucanase and an alkaline cellobiohydrolase. We observed higher activities of both cellulose degradation and biostoning by the co-expression of an endoglucanase and a cellobiohydrolase than the activities obtained by the expression of only endoglucanase or cellobiohydrolase. This study makes the process of engineering expression of multiple genes easier in T. reesei.

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Heterologous protein expression in Trichoderma reesei using the cbhII promoter

Meng

Wei

Wang

2013

Plasmid

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Specific Characteristics of an Endoglucanase RCE1 from Rhizopus Oryzae in the Treatment of the Dyed Cotton Fabrics

Cited by 5 publications

References 9 publications

Purification and Characterization of a New Family 45 Endoglucanase, STCE1, from Staphylotrichum coccosporum and Its Overproduction in Humicola insolens

Purification and Characterization of a New Family 45 Endoglucanase, STCE1, from Staphylotrichum coccosporum and Its Overproduction in Humicola insolens

Construction of a promoter collection for genes co-expression in filamentous fungus Trichoderma reesei

Heterologous protein expression in Trichoderma reesei using the cbhII promoter

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