Specific cleavage of ribosomal RNA and mRNA during victorin‐induced apoptotic cell death in oat

Hoat, Trinh Xuan; Nakayashiki, Hitoshi; Tosa, Yukio; Mayama, Shigeyuki

doi:10.1111/j.1365-313x.2006.02752.x

Cited by 20 publications

(25 citation statements)

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“…Circles denote total germination as in b. Asterisks in b and d indicate differences between non-aged control seeds and Aged or dead seeds at a level of P \ 0.01 (one-way ANOVA) (Bray and Chow 1976;Boubriak et al 2000;Brockelhurst and Fraser 1980;Cheah and Osborne 1978;Osborne 2000). The final, or execution, phase of PCD is characterised by inter-nucelosomal DNA fragmentation (Elbaz et al 2002;Hengartner 2000;Thomas and Franklin-Tong 2004) and can include RNA degradation (Hoat et al 2006;Perrin et al 2004). It has been reported occasionally for apoptotic animal cells that rRNA remained intact (Houge and Doskeland 1996) and conversely, rRNA fragmentation occurred without DNA laddering (Samali et al 1997).…”

Section: Discussionmentioning

confidence: 97%

Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing

et al. 2010

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The germination of viable seeds is the basis for new plant growth and development. Seeds lose viability during storage, but the biochemical mechanisms of seed death are not fully understood. This study aimed to investigate degradation patterns of nucleic acids during seed ageing and subsequent water uptake. Seeds of Pisum sativum L. were artificially aged at 50°C and 12% seed water content (WC). Nucleic acids degradation was studied during ageing and during imbibition of four seed lots with differential viability from highly viable to dead. As seeds lost viability during ageing, DNA was gradually degraded into internucleosomal fragments, resulting in 'DNA laddering', in conjunction with disintegration of 18S and 28S rRNA bands. During imbibition, non-aged controls had high levels of DNA and RNA integrity through to radicle protrusion. In an aged seed lot with 85% total germination (TG) DNA fragmentation decreased upon imbibition probably due to nucleosome degradation, while rRNA integrity did not improve. In an aged seed lot with 44% TG, neither DNA nor rRNA integrity improved upon imbibition. Dead seeds showed DNA degradation as laddering throughout imbibition along with extensive degradation of rRNA. We present a model in which interlinked programmed and nonprogrammed events contribute to seed ageing, and suggest that protection of nucleic acids during ageing is key to seed longevity.

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Section: Discussionmentioning

confidence: 97%

Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing

et al. 2010

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“…ZnCl 2 functions as a Ca 2+ antagonist preventing calcium metabolism, and inhibits victorin-induced apoptotic cell death in oat (Hoat et al 2006). To examine the effect of ZnCl 2 on necrotic cell death in wheat hybrids, we treated seedlings showing the three types of hybrid necrosis with ZnCl 2 .…”

Section: Alleviation Of Necrotic Symptoms Via Zinc Chloride Treatmentmentioning

confidence: 99%

Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives

et al. 2015

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Hybrid necrosis is a well-known reproductive isolation mechanism in plant species, and an autoimmune response is generally considered to trigger hybrid necrosis through epistatic interaction between disease resistance-related genes in hybrids. In common wheat, the complementary Ne1 and Ne2 genes control hybrid necrosis, defined as type I necrosis. Two other types of hybrid necrosis (type II and type III) have been observed in interspecific hybrids between tetraploid wheat and Aegilops tauschii. Another type of hybrid necrosis, defined here as type IV necrosis, has been reported in F1 hybrids between Triticum urartu and some accessions of Triticum monococcum ssp. aegilopoides. In types I, III and IV, cell death occurs gradually starting in older tissues, whereas type II necrosis symptoms occur only under low temperature. To compare comprehensive gene expression patterns of hybrids showing growth abnormalities, transcriptome analysis of type I and type IV necrosis was performed using a wheat 38k oligo-DNA microarray. Defense-related genes including many WRKY transcription factor genes were dramatically up-regulated in plants showing type I and type IV necrosis, similarly to other known hybrid abnormalities, suggesting an association with an autoimmune response. Reactive oxygen species generation and necrotic cell death were effectively inhibited by ZnCl2 treatment in types I, III and IV necrosis, suggesting a significant association of Ca(2+) influx in upstream signaling of necrotic cell death in wheat hybrid necrosis.

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“…In plants, ribosomal RNA is specifically cleaved during apoptotic cell death induced by victorin in oat cells [10]. However, rRNA cleavage does not appear as a general feature associated with apoptosis [21].…”

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confidence: 99%

“…Under these conditions, alamethicin induced specific rRNA cleavage. Interestingly, rRNA cleavage has previously been shown to occur in plant and animal cell lines during apoptosis [10] [11]. Apoptosis is a form of programmed cell death (PCD) 2 ) that plays fundamental roles in tissue homeostasis and stress responses [12].…”

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confidence: 99%

The Peptaibol Alamethicin Induces an rRNA‐Cleavage‐Associated Death in Arabidopsis thaliana

Rippa

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Michel

et al. 2007

Chemistry & Biodiversity

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The plant-metabolic response to amphipathic peptides produced by the soil fungi of the genus Trichoderma remains largely unknown. The present investigation was undertaken to examine the death process in alamethicin-treated Arabidopsis thaliana plantlets. The rapid death triggered by alamethicin (at 50 microM) was shown to be associated with protein-synthesis arrest and with specific cleavage of 18S and 25S ribosomal RNA. The use of an inhibitor of nitric oxide (NO) synthases and of an NO scavenger suggested that rRNA cleavage was suppressed by NO. Experiments conducted with a synthetic alamethicin analogue, in which all alpha-aminoisobutyric acid (Aib) residues have been replaced by leucine moieties, showed that the non-coded residues are essential for the ability of the peptaibol to induce rRNA cleavage in Arabidopsis. Our data indicate that further investigations on the mode of action of alamethicin in planta could be of great interest to study the death-signaling pathway associated with rRNA degradation in plants.

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Specific cleavage of ribosomal RNA and mRNA during victorin‐induced apoptotic cell death in oat

Cited by 20 publications

References 84 publications

Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing

Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing

Comparison of gene expression profiles and responses to zinc chloride among inter- and intraspecific hybrids with growth abnormalities in wheat and its relatives

The Peptaibol Alamethicin Induces an rRNA‐Cleavage‐Associated Death in Arabidopsis thaliana

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