Specific Contributions of Histone Tails and their Acetylation to the Mechanical Stability of Nucleosomes

Brower-Toland, Brent D.; Wacker, David A.; Fulbright, Robert M.; Lis, John T.; Kraus, W. Lee; Wang, Michelle D.

doi:10.1016/j.jmb.2004.11.056

Cited by 189 publications

(214 citation statements)

References 48 publications

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“…10(b)]. Fiber extension was monitored, with specific attention paid to sudden increases in overall extension (an indication of a possible nucleosome disruption event 34,35,[58][59][60]. Three consecutive extensions of the same fiber are shown in Fig.…”

Section: B Resultsmentioning

confidence: 99%

Thin-foil magnetic force system for high-numerical-aperture microscopy

Fisher

Cribb

Desai

et al. 2006

Review of Scientific Instruments

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Forces play a key role in a wide range of biological phenomena from single-protein conformational dynamics to transcription and cell division, to name a few. The majority of existing microbiological force application methods can be divided into two categories: those that can apply relatively high forces through the use of a physical connection to a probe and those that apply smaller forces with a detached probe. Existing magnetic manipulators utilizing high fields and high field gradients have been able to reduce this gap in maximum applicable force, but the size of such devices has limited their use in applications where high force and high-numerical-aperture (NA) microscopy must be combined. We have developed a magnetic manipulation system that is capable of applying forces in excess of 700 pN on a 1 μm paramagnetic particle and 13 nN on a 4.5 μm paramagnetic particle, forces over the full 4π sr, and a bandwidth in excess of 3 kHz while remaining compatible with a commercially available high-NA microscope objective. Our system design separates the pole tips from the flux coils so that the magnetic-field geometry at the sample is determined by removable thin-foil pole plates, allowing easy change from experiment to experiment. In addition, we have combined the magnetic manipulator with a feedback-enhanced, high-resolution (2.4 nm), highbandwidth (10 kHz), long-range (100 μm xyz range) laser tracking system. We demonstrate the usefulness of this system in a study of the role of forces in higher-order chromosome structure and function.

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Section: B Resultsmentioning

confidence: 99%

Thin-foil magnetic force system for high-numerical-aperture microscopy

Fisher

Cribb

Desai

et al. 2006

Review of Scientific Instruments

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“…Aliquots of purified histones were stored in À 80°C at a final concentration of 2.7 mM. Nucleosomes were assembled on the Widom 601 nucleosome-positioning element 49 by salt dialysis [10][11][12][13]15,16,20,[50][51][52][53] . For the mechanical displacement assay, nucleosomes were assembled on a 764 bp template at a molar ratio of 1.25:1.00 of histone octamer to DNA.…”

Section: Methodsmentioning

confidence: 99%

DNA Looping Mediates Nucleosome Transfer

Brennan

Forties²,

Patel

et al. 2017

Biophysical Journal

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Proper cell function requires preservation of the spatial organization of chromatin modifications. Maintenance of this epigenetic landscape necessitates the transfer of parental nucleosomes to newly replicated DNA, a process that is stringently regulated and intrinsically linked to replication fork dynamics. This creates a formidable setting from which to isolate the central mechanism of transfer. Here we utilized a minimal experimental system to track the fate of a single nucleosome following its displacement, and examined whether DNA mechanics itself, in the absence of any chaperones or assembly factors, may serve as a platform for the transfer process. We found that the nucleosome is passively transferred to available dsDNA as predicted by a simple physical model of DNA loop formation. These results demonstrate a fundamental role for DNA mechanics in mediating nucleosome transfer and preserving epigenetic integrity during replication.

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“…The analysis of nucleosome thermodynamics has been daunting, due to the complexity and high stability of the system under investigation. Developments in single molecule approaches appear the most promising [37] [38] [39].…”

Section: Chaperone -Histone Interactions and Implications For Nucleosmentioning

confidence: 99%