Specific degradation of hyaluronic acid and its rheological properties

Gura, E.; Hückel, M.; Müller, Peter

doi:10.1016/s0141-3910(97)00194-8

Cited by 46 publications

(34 citation statements)

References 12 publications

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“…In fact, the rheological profiles show that HPO 4 2− increases the viscosity of the SH solution to 77.68 Pa·s, while H 2 PO 4 − lowers (19.75) and PO 4 3− dramatically breaks down the flow curves. We recall that the rheological properties of hyaluronate strongly depend on the pH in a non-linear correlation (Gatej, Popa, & Rinaudo, 2005;Gibbs, Merrill, & Smith, 1968;Gura, Hückel, & Müller, 2003;Maleki, Kjøniksen, & Nyström, 2008). In particular for hyaluronic acid the complex viscosity reaches a maximum value at about pH = 2.5, sided by two remarkable drops in acid (pH = 1.6) and mid-acid conditions (pH = 3.34) (Gatej et al, 2005).…”

Section: Tablementioning

confidence: 94%

Specific ion effects in polysaccharide dispersions

Tatini

Sarri

Maltoni

et al. 2017

Carbohydrate Polymers

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The specific effects induced by some strong electrolytes or neutral cosolutes on aqueous mixtures of guar gum (GG), sodium alginate (SA) and sodium hyaluronate (SH) were studied through rheology and DSC experiments. The results are discussed in terms of changes in the polymer conformation, structure of the network and hydration properties. This study is also aimed at controlling the viscosity of the aqueous mixturesfor application in green formulations to be used as fracturing fluids for shale gas extraction plants.

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Section: Tablementioning

confidence: 94%

Specific ion effects in polysaccharide dispersions

Tatini

Sarri

Maltoni

et al. 2017

Carbohydrate Polymers

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“…37,38 For this approach, HA was dissolved in 200 mM NaCl solution and diluted to concentrations of 1, 0.75, 0.5, 0.25, and 0.125 mg/mL. In the first step, the Ubbelohde viscometer equipped with a CC-130A Visco 3 water bath (Huber SE, Berching, Germany) at 25°C was used to measure the run time (t c ) of the samples through the capillary (diameter 0.63 mm).…”

Section: Degradation Of Hamentioning

confidence: 99%

Development and characterization of magnetic iron oxide nanoparticles with a cisplatin-bearing polymer coating for targeted drug delivery

Unterweger¹,

Tietze²,

Janko³

et al. 2014

IJN

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A highly selective and efficient cancer therapy can be achieved using magnetically directed superparamagnetic iron oxide nanoparticles (SPIONs) bearing a sufficient amount of the therapeutic agent. In this project, SPIONs with a dextran and cisplatin-bearing hyaluronic acid coating were successfully synthesized as a novel cisplatin drug delivery system. Transmission electron microscopy images as well as X-ray diffraction analysis showed that the individual magnetite particles were around 4.5 nm in size and monocrystalline. The small crystallite sizes led to the superparamagnetic behavior of the particles, which was exemplified in their magnetization curves, acquired using superconducting quantum interference device measurements. Hyaluronic acid was bound to the initially dextran-coated SPIONs by esterification. The resulting amide bond linkage was verified using Fourier transform infrared spectroscopy. The additional polymer layer increased the vehicle size from 22 nm to 56 nm, with a hyaluronic acid to dextran to magnetite weight ratio of 51:29:20. A maximum payload of 330 μg cisplatin/mL nanoparticle suspension was achieved, thus the particle size was further increased to around 77 nm with a zeta potential of −45 mV. No signs of particle precipitation were observed over a period of at least 8 weeks. Analysis of drug-release kinetics using the dialysis tube method revealed that these were driven by inverse ligand substitution and diffusion through the polymer shell as well as enzymatic degradation of hyaluronic acid. The biological activity of the particles was investigated in a nonadherent Jurkat cell line using flow cytometry. Further, cell viability and proliferation was examined in an adherent PC-3 cell line using xCELLigence analysis. Both tests demonstrated that particles without cisplatin were biocompatible with these cells, whereas particles with the drug induced apoptosis in a dose-dependent manner, with secondary necrosis after prolonged incubation. In conclusion, combination of dextran-coated SPIONs with hyaluronic acid and cisplatin represents a promising approach for magnetic drug targeting in the treatment of cancer.

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“…Capillary viscometry-based Mark-Houwink equation was used (Gura et al 1998) to characterize hyaluronan fragments after being undergone to ultrasonical degradation.…”

Section: Hyaluronan Susceptibility To Peroxynitrite Oxidative Actionmentioning

confidence: 99%

Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation

Hrabárová¹,

Juránek²,

Šoltés³

2011

gpb

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Abstract. Current understanding on the role of peroxynitrite in etiology and pathogenesis of some human diseases, such as cardio-vascular diseases, stroke, cancer, inflammation, neurodegenerative disorders, diabetes mellitus and diabetic complications has recently led to intensive investigation of peroxynitrite involvement in physiology and pathophysiology.Mechanism of cytotoxic effects of peroxynitrite involve its reactions with lipids, DNA/RNA, proteins, and polysaccharides, thus triggering cellular responses ranging from subtle changes of cell functioning to severe oxidative damage of the affected macromolecules leading to necrosis or apoptosis. The present work is aimed at providing a brief overview of i) peroxynitrite biosynthesis and reaction pathways in vivo, ii) its synthetic preparation in vitro, and iii) to reveal its potential damaging role in vivo, on actions studied via monitoring in vitro hyaluronan degradation. The complex biochemical behavior of peroxynitrite is determined by a number of variables, such as chemistry of the reaction itself, depending mostly on the involvement of conformational structures of different energy states, concentration of the species involved, content of reactive intermediates and trace transition metal ions, contribution of carbon dioxide, presence of trace organics, and by the reaction kinetics. Recently, in vitro studies of oxidative cleavage of hyaluronan have, in fact, been the subject of growing interest. Here we also describe our experimental set-up for studying peroxynitrite-mediated degradation of hyaluronan, a system, which may be suitable for testing prospective pharmacological substances.

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Specific degradation of hyaluronic acid and its rheological properties

Cited by 46 publications

References 12 publications

Specific ion effects in polysaccharide dispersions

Specific ion effects in polysaccharide dispersions

Development and characterization of magnetic iron oxide nanoparticles with a cisplatin-bearing polymer coating for targeted drug delivery

Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation

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Specific degradation of hyaluronic acid and its rheological properties

Cited by 46 publications

References 12 publications

Specific ion effects in polysaccharide dispersions

Specific ion effects in polysaccharide dispersions

Development and characterization of magnetic iron oxide nanoparticles with a cisplatin-bearing polymer coating for targeted drug delivery

Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation

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Pro-oxidative effect of peroxynitrite regarding biological systems: a special focus on high-molar-mass hyaluronan degradation