Specific Detection and Localization of Microsporidian Parasites in Invertebrate Hosts by Using
            <i>In Situ</i>
            Hybridization

Dubuffet, Aurore; Smith, Judith E.; Solter, Leellen F.; Perotti, M. Alejandra; Braig, Henk R.; Dunn, Alison M.

doi:10.1128/aem.02699-12

Cited by 18 publications

(11 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The life cycle of numerous Dictyocoela species is not known. In two hosts, Gammarus duebeni and G. roeselii, Dictyocoela roeselum, D. duebenum and D. muelleri are known to be vertically transmitted: they infect oocytes, and, therefore, are transmitted to most embryos [30][31][32]. These species induce low virulence [33] as well as sexratio distortion in their host populations.…”

Section: Introductionmentioning

confidence: 99%

Dictyocoela microsporidia diversity and co-diversification with their host, a gammarid species complex (Crustacea, Amphipoda) with an old history of divergence and high endemic diversity

et al. 2020

View full text Add to dashboard Cite

Background Although the processes of co-evolution between parasites and their hosts are well known, evidence of co-speciation remains scarce. Microsporidian intracellular parasites, due to intimate relationships with their hosts and mixed mode of transmission (horizontal but also vertical, from mother to offspring), may represent an interesting biological model for investigating co-speciation. Amphipod crustaceans, especially gammarids, are regular hosts of microsporidian parasites, in particular the Dictyocoela spp., which have so far been found limited to these amphipods and are known to use a vertical mode of transmission. The amphipod genus Gammarus has a diversification history spanning the last 50–60 Mya and an extensive cryptic diversity in most of the nominal species. Here, we investigated the degree of co-diversification between Dictyocoela and Gammarus balcanicus, an amphipod with high degrees of ancient cryptic diversification and lineage endemism, by examining the genetic diversity of these parasites over the entire geographic range of the host. We hypothesised that the strong host diversification and vertical transmission of Dictyocoela would promote co-diversification. Results Using the parasite SSU rDNA as a molecular marker, analyzing 2225 host specimens from 88 sites covering whole host range, we found 31 haplogroups of Dictyocoela, 30 of which were novel, belonging to four Dictyocoela species already known to infect other Gammarus spp. The relationships between Dictyocoela and gammarids is therefore ancient, with the speciation in parasites preceding those of the hosts. Each novel haplogroup was nevertheless specific to G. balcanicus, leaving the possibility for subsequent co-diversification process during host diversification. A Procrustean Approach to Co-phylogeny (PACo) analysis revealed that diversification of Dictyocoela was not random with respect to that of the host. We found high degrees of congruence between the diversification of G. balcanicus and that of Dictyocoela roeselum and D. muelleri. However, we also found some incongruences between host and Dictyocoela phylogenies, e.g. in D. duebenum, probably due to host shifts between different G. balcanicus cryptic lineages. Conclusion The evolutionary history of Dictyocoela and Gammarus balcanicus represents an example of an overall host-parasite co-diversification, including cases of host shifts.

show abstract

Section: Introductionmentioning

confidence: 99%

Dictyocoela microsporidia diversity and co-diversification with their host, a gammarid species complex (Crustacea, Amphipoda) with an old history of divergence and high endemic diversity

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Pathogens of this group can remain latent without causing visible symptoms in hosts (Bjørnson 2008). This can be perpetuated for several generations through the possibility of vertical transmission, when infection is transmitted from parents to offspring and horizontal when it occurs through contact between individuals, which may be favored by the confinement environment of mass productions (Dubuffet et al 2013). Recent studies have shown a significant effect of the breeding laboratories' ambient temperature on the intensity of the endoparasites Nosema spp.…”

Section: Resultsmentioning

confidence: 99%

Infection by the microsporidium of Clado Nosema/Vairimorpha in pupal parasitoids

Paes

Carvalho

Souza

et al. 2019

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The sugarcane borer, Diatraea saccharalis is one of the hosts more used for parasitoid pupal multiplication in Brazil. The parasitoids pupal of Trichospilus diatraeae and Palmistichus elaeisis are generalist natural enemies with potential to suppress populations of diverse families of lepidopteran pests. The success in the utilization of these natural enemies in the field is directly related to the capacity of search of the host, this capacity might be affected by the presence of the pathogens. In this context, the aim of this essay was to detect the presence of intracellular parasites of Phylum Microsporidia. These pathogens may cause morphological and behavioral alterations. The presence of infection was verified by microscopy and was confirmed by amplification of region small subunit (SSU) of ribosomal rNA using universal primers for microsporidia of Nosema sp. The purified PCR products were submitted to sequencing, and the sequences that had been obtained were edited and aligned with the sequences in a genbank database. In this way, it was possible to verify the presence of intracellular parasites in T. diatraeae, P. elaeisis and D. saccharalis pertaining to Clade Nosema/Vairimorpha. However, this is the first one report about detection of the microsporidia in the parasitoids T. diatraeae and P. elaeisis.

show abstract

“…Dubuffet et al ., designed fluorescenct in situ hybridization probes for two distinct microsporidian clades and demonstrated their efficacy in detecting and studying the vertical transmission of two microsporidia [22]. These methods are ideal for microsporidian visualization but not applicable for display protein location during the development of the parasite.…”

Section: Discussionmentioning

confidence: 99%

Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells

et al. 2017

View full text Add to dashboard Cite

Microsporidia are eukaryotic, unicellular parasites that have been studied for more than 150 years. These organisms are extraordinary in their ability to invade a wide range of hosts including vertebrates and invertebrates, such as human and commercially important animals. A lack of appropriate labeling methods has limited the research of the cell cycle and protein locations in intracellular stages. In this report, an easy fluorescent labeling method has been developed to mark the proliferative and sporogonic phases of microsporidia Nosema bombycis in host cells. Based on the presence of chitin, Calcofluor White M2R was used to label the sporogonic phase, while β-tubulin antibody coupled with fluorescence secondary antibody were used to label the proliferative phase by immunofluorescence. This method is simple, efficient and can be used on both infected cells and tissue slices, providing a great potential application in microsporidia research.

show abstract

Specific Detection and Localization of Microsporidian Parasites in Invertebrate Hosts by Using In Situ Hybridization

Cited by 18 publications

References 40 publications

Dictyocoela microsporidia diversity and co-diversification with their host, a gammarid species complex (Crustacea, Amphipoda) with an old history of divergence and high endemic diversity

Dictyocoela microsporidia diversity and co-diversification with their host, a gammarid species complex (Crustacea, Amphipoda) with an old history of divergence and high endemic diversity

Infection by the microsporidium of Clado Nosema/Vairimorpha in pupal parasitoids

Easy labeling of proliferative phase and sporogonic phase of microsporidia Nosema bombycis in host cells

Contact Info

Product

Resources

About