Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay

Liu, Chunyan; Song, Hui-Qun; Zhang, Renli; Chen, Mu-Xin; Xu, Min-Jun; Ai, Lin; Chen, Xiaoguang; Zhan, Xi; Liang, Shuli; Yuan, Zihao; Lin, Rui-Qing; Zhu, Xingquan

doi:10.1016/j.mcp.2011.04.002

Cited by 23 publications

(26 citation statements)

References 22 publications

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“…Research has not, however, focused solely on these major diseases. Of the 17 neglected tropical diseases (NTDs) recognized by WHO [21], 14 have been studied using LAMP, these being dengue [14,22], rabies [23], buruli ulcer [24,25], leprosy [26], Chagas disease [27,28], human African trypanosomiasis [29], leishmaniasis [30,31], cysticercosis [32], echinococcosis [33], foodborne trematode infections [34], lymphatic filariasis [35], schistosomiasis [36], soil-transmitted helminthiases [37], and yaws [38]. The three exceptions are trachoma, onchocerciasis, and dracunculiasis.…”

Section: Developmental Prospects Of New Lamp Reagentsmentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Mori¹,

Kanda²,

Notomi³

2013

Journal of Infection and Chemotherapy

237

175

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Loop-mediated isothermal amplification (LAMP) is an established technology that continues to attract the attention of researchers in many fields. Research and development efforts on LAMP technology in recent years have focused on two major areas; first, the study of its clinical application as an approved in vitro diagnostics tool in Japan and certain other countries; and second, research aimed at further simplifying the LAMP test process. This review provides an overview of the status of LAMP on these two topics by summarizing research work conducted, in the main, after our previous review article.

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Section: Developmental Prospects Of New Lamp Reagentsmentioning

confidence: 99%

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Mori¹,

Kanda²,

Notomi³

2013

Journal of Infection and Chemotherapy

237

175

View full text Add to dashboard Cite

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“…A loop-mediated isothermal DNA amplification (LAMP) technique has also been used to detect A . cantonensis in invasive snail species [ 12 , 13 ] and presents several advantages when compared to PCR. For instance, the LAMP technique does not require special equipment; amplification of the target DNA can be performed in a water bath or heat block and the end-point analysis can be achieved by visual inspection directly in the reaction tube.…”

Section: Introductionmentioning

confidence: 99%

“…For instance, the LAMP technique does not require special equipment; amplification of the target DNA can be performed in a water bath or heat block and the end-point analysis can be achieved by visual inspection directly in the reaction tube. The LAMP assay has already been applied to the detection and identification of other parasites of humans and animals [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Intermediate Hosts of Angiostrongylus cantonensis in Tenerife, Spain

et al. 2015

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The nematode Angiostrongylus cantonensis is the causative agent of human angiostrongyliasis, the main clinical manifestation of which is eosinophilic meningitis. Although this parasite has been found recently in its definitive rat host in Tenerife (Canary Islands, Spain), showing a widespread distribution over the north-east part of the island, there are no available data regarding which snail and/or slug species are acting as intermediate hosts on this island. Consequently, the objective of this work was to determine the possible role of three mollusc species, Plutonia lamarckii, Cornu aspersum and Theba pisana, as intermediate hosts of A. cantonensis in Tenerife. Between 2011 and 2014, 233 molluscs were collected from five biotopes where rats had been found previously to harbor either adult worms or antibodies against A. cantonensis, and the identification was carried out on the basis of morphological features and a LAMP technique. The prevalence of A. cantonensis larvae in the mollusc samples, based on morphological identification, was 19.3%, whereas 59 out of the 98 individuals (60.2%) analyzed by LAMP were positive. Positive results were obtained for the three mollusc species analyzed and two of the positive samples, both obtained from P. lamarckii, were confirmed as positive by 18S rRNA and ITS1 PCR. Sequence analysis of 18S rRNA PCR products showed 100% similarity with previously published A. cantonensis sequences. These results may be relevant from a public health point of view, since all the biotopes from which the samples were obtained were in inhabited areas or areas with human activity, but it is also important from the perspective of a possible transmission to other accidental hosts, such as dogs and horses, animals that are present in some of the areas analyzed.

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“…In America, this association has been demonstrated in the United States, Cuba, Jamaica, Bahamas, Puerto Rico, Dominican Republic, Haiti, Costa Rica, Brazil, Ecuador, Venezuela, and Argentina (5,12,13). Despite this, there is a lack of information on the real impact of this invasive mollusk on nematode dynamics, more so when there are no practical and effective diagnostic methods in countries where the snail plague is recent or the epidemiological and clinical impact of the nematodes, especially the genera Angiostrongylus, is unknown, as is the case in Colombia (5,14).Therefore, the objective of this study was to determine the presence and prevalence of Strongylida nematodes in Achatina fulica of the Valle del Cauca, Colombia, mainly of those nematodes potentially pathogenic for humans.…”

Section: Introductionmentioning

confidence: 94%

Prevalencia de parásitos nematodos Strongylidos asociados al Caracol africano, Achatina fulica, en el Valle del Cauca, Colombia

Patiño‐Montoya

Giraldo

2017

Rev MVZ Córdoba

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Prevalencia de parásitos nematodos Strongylidos asociados al ABSTRACTObjectives. To establish the presence and prevalence of Strongylida nematode parasites in Achatina fulica in the Valle del Cauca, especially of nematodes that are potentially pathogenic for humans. Materials and methods. A. fulica individuals were collected in nine cities of the Valle del Cauca, Colombia. Direct visual examination was used to identify A. fulica parasites. Nematodes were separated from tissue or collected from mucus, washed in saline solution, and fixed in a hot AFA solution. Samples were mounted in glycerine and observed under the microscope. Results. The general nematode parasite prevalence was 35% in 2013. The city with highest prevalence during 2013 was Cartago (60%), following by Buenaventura (42.9%) and Cali (33%), while during 2014 were Cali (30%) and Buenaventura (30%). The Strongylida nematodes registered were classified in three genera: Angiostrongylus (14.7% prevalence), Aelurostrongylus (2.6%),and Strongyluris (2.6%). The city with highest positive records of Angiostrongylus was Cali during 2014 and Aelurostrongylus was Buenaventura during 2013. Strongyluris genus was recorded only in Cali during 2013, with a prevalence of 11%. Of the nine evaluated cities, five has presence of Angiostrongylus. Conclusions. Three genera of Strongylida nematode were recorded associated with A. fulicas specimens in the Valle del Cauca during 2013 and 2014. Therefore, the role that A. fulica and native mollusk species could be playing in the life cycle of these parasites at the local level should not underestimated.

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Specific detection of Angiostrongylus cantonensis in the snail Achatina fulica using a loop-mediated isothermal amplification (LAMP) assay

Cited by 23 publications

References 22 publications

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Loop-mediated isothermal amplification (LAMP): recent progress in research and development

Intermediate Hosts of Angiostrongylus cantonensis in Tenerife, Spain

Prevalencia de parásitos nematodos Strongylidos asociados al Caracol africano, Achatina fulica, en el Valle del Cauca, Colombia

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