Specific detection of carcinoembryonic antigen-expressing tumor cells in bone marrow aspirates by polymerase chain reaction.

Gerhard, Markus; Juhl, Hartmut; Kalthoff, Holger; Schreiber, Hans; Wagener, Christoph; Neumaier, Michael

doi:10.1200/jco.1994.12.4.725

Cited by 333 publications

(218 citation statements)

References 22 publications

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“…Usually, the limit of detection with such a technique is one carcinoma cell per 4-5 x 105 mononuclear cells. Data derived from several studies using RT-PCR techniques to identify carcinoma cells in peripheral blood samples show that the lower limit of detection is of the same order as found in this study (Smith et al, 1991;Matsumura and Tarin 1992;Mattano et al, 1992;Hardingham et al, 1993;Datta et British Journal of Cancer (1997) 76(1), 29-35 al, 1994;Gerhard et al, 1994;Israeli et al, 1994;Seiden et al, 1994;Burchill et al, 1995;Peter et al, 1995).…”

Section: Discussionsupporting

confidence: 74%

A quantitative reverse transcriptase polymerase chain reaction-based assay to detect carcinoma cells in peripheral blood

Helfrich¹,

tenPoele²,

Meersma³

et al. 1997

Br J Cancer

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Summary The presence of tumour cells in the circulation may predict disease recurrence and metastasis. To improve on existing methods of cytological or immunocytological detection, we have developed a sensitive and quantitative technique for the detection of carcinoma cells in blood, using the reverse transcriptase polymerase chain reaction (RT-PCR) identifying transcripts of the pancarcinoma-associated tumour marker EGP-2 (KSA or 17-lA antigen). The amount of EGP2 mRNA was quantified using an internal recombinant competitor RNA standard with known concentration and which is both reversely transcribed and co-amplified in the same reaction, allowing for a reliable assessment of the initial amount of EGP2 mRNA in the sample. Calibration studies, seeding blood with MCF-7 breast carcinoma cells, showed that the assay can detect ten tumour cells among 1.0 x 106 leucocytes. The PCR assay revealed that normal bone marrow expresses low levels of EGP2 mRNA, although immunocytochemistry with the anti-EGP2 MAb MOC31 could not identify any positively stained cell. Analyses using this RT-PCR assay may prove to have applications to the assessment of circulating tumour cells in clinical samples.

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Section: Discussionsupporting

confidence: 74%

A quantitative reverse transcriptase polymerase chain reaction-based assay to detect carcinoma cells in peripheral blood

Helfrich¹,

tenPoele²,

Meersma³

et al. 1997

Br J Cancer

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“…Among these markers are cytokeratin-19 (CK19), mammaglobin, maspin, and carcinoembryonic antigen (CEA) [2][3][4][5][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Detection of occult HER2 mRNA-positive tumor cells in the peripheral blood of patients with operable breast cancer: evaluation of their prognostic relevance

Apostolaki

Perraki

Kallergi

et al. 2008

Breast Cancer Res Treat

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“…Inc., Palo Alto, CA, USA) according to the manufacturer's protocol and then stored at À201C. A CEA-specific oligonucleotide primer was designed based on that described by Gerhard et al (1994) as follows: sense, 5 0 -TGTCGGCATCAT GATTGG-3 0 and antisense, 5 0 -GCAAATGCTTTAAGGAAGAAGC-3 0 . The donor and acceptor probe sequences for CEA identification were 5 0 -CCTGAAATGAAGAAACTACACCAGGGC-fluorescein and 5 0 -LC-Red640-GCTATATCAGAGCAACCCCAACCAGC-phosphorylation.…”

Section: Real-time Rt -Pcrmentioning

confidence: 99%

“…Some authors have proven that RT -PCR can detect lymph node micrometastasis in gastrointestinal tract or breast cancers (Mori et al, 1995;Matsumoto et al, 2002). Circulating cancer cells in the bone marrow or peripheral blood and free cancer cells in the peritoneal lavage fluid have been detected by RT -PCR (Gerhard et al, 1994;Burchill et al, 1995;Marutsuka et al, 2003). Thus, RT -PCR-based techniques can detect a minuscule number of occult cancer cells.…”

mentioning

confidence: 99%

Lymphatic invasion using D2-40 monoclonal antibody and its relationship to lymph node micrometastasis in pN0 gastric cancer

et al. 2005

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The monoclonal antibody D2-40 is a specific lymphatic endothelial markers and D2-40 staining have been applicable to evaluate lymphatic invasion in various malignant neoplasms. In the present study, we investigated lymph node micrometastasis determined by immunohistochemistry (IHC) and reverse transcription -polymerase chain reaction (RT -PCR) in all dissected lymph nodes obtained from 80 patients with node-negative gastric cancer, and analysed the relationship between micrometastasis and clinicopathological findings including lymphatic invasion of the resected primary tumour using D2-40 immunohistochemical staining. The incidence of micrometastasis determined by IHC and RT -PCR was 11.3% (nine out of 80) and 31.3% (25 out of 80), respectively. Although haematoxylin -eosin (HE) staining revealed lymphatic invasion in 11.3% (nine out of 80) of patients, D2-40 staining uncovered new invasion in 23.8% (19 out of 80) of patients. In the diagnosis of HE and D2-40 staining, the incidence of micrometastasis was significantly higher in patients with lymphatic invasion than in those without lymphatic invasion (P ¼ 0.0150 and Po0.0001, respectively). Micrometastasis correlated more closely with D2-40 than with HE staining. We demonstrated a high incidence of micrometastasis and lymphatic invasion and a correlation between them even in pN0 gastric cancer. When planning less invasive treatment, the presence of such occult cancer cells should be considered.

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Specific detection of carcinoembryonic antigen-expressing tumor cells in bone marrow aspirates by polymerase chain reaction.

Cited by 333 publications

References 22 publications

A quantitative reverse transcriptase polymerase chain reaction-based assay to detect carcinoma cells in peripheral blood

A quantitative reverse transcriptase polymerase chain reaction-based assay to detect carcinoma cells in peripheral blood

Detection of occult HER2 mRNA-positive tumor cells in the peripheral blood of patients with operable breast cancer: evaluation of their prognostic relevance

Lymphatic invasion using D2-40 monoclonal antibody and its relationship to lymph node micrometastasis in pN0 gastric cancer

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