Specific Determination of Bromate in Bread by Ion Chromatography with ICP-MS.

Abstract

A sensitive method for detecting bromate in bread by ion chromatography with inductivelycoupled plasma mass spectrometry (IC/ICP-MS) was developed. Bromate was extracted from bread with water. The clean-up procedure included a 0.2 mm filter, a C 18 cartridge for defatting, a silver cartridge to remove halogen anions, a centrifugal ultrafiltration unit to remove proteins, and a cation-exchange cartridge to remove silver ions. A 500 mL sample solution was applied to IC/ ICP-MS. The detection limit and the quantitation limit of bromate in the solution were 0.3 ng/mL and 1.0 ng/mL, expressed as HBrO 3 , respectively, which corresponded to 2 ng/g and 5 ng/g, respectively, in bread. Recovery of bromate was about 90῍, and the CV was about 2῍. Based on the detection limit in solution and recovery from bread, the detection limit of bromate in bread was estimated to be 2 ng/g.

Development of Indicator for on-Site Detection of Bromate in Some Bread Samples in Abeokuta Metropolis

“…The mean content of KBrO 3 (Table 2) found in the samples collected in bakeries A, B, C, D, and E were 6.29±0. The concentration of KBrO 3 in bread samples obtained in this study is similar to the 3.60 and 9.20 μg/g for the lowest and highest level of KBrO 3 as reported in Gwagwalada, Nigeria [11].It is also similar to 3.70 and 12.10 μg/g as the lowest and highest level of KBrO 3 found in bread samples consumed in Kaduna metropolis respectively [23]. The concentration of KBrO 3 in bread samples obtained in this study was above the USFDA permissible limit, which is an indication that the brands of bread samples are unsafe for human consumption.…”

“…Several analytical techniques had been developed for the quantitative determination of KBrO 3 in bread which involve the use of sophisticated and expensive equipment. They include: Ion chromatography method with ICP-MS [10,11], Redox titrimetric method [12], High Performance Liquid Chromatography [13,14], and spectroscopic methods [15,16,17]. However, there are several limitations associated with many of these currently available methods, such as being time consuming and requiring complicated instrumentation.…”

Atomic spectrometry update. Clinical and biological materials, foods and beverages

“…An LOD of 0.4 mg g 21 was reported for a 30 mg dry sample and the method was validated by analysing TORT-1 CRM. To determine BrO 3 2 Akiyama et al 219 developed an unusually prolonged procedure that included extraction with water, a 0.2 mm filter and thenx removal of fat, halide anions and protein with a C 18 cartridge, a silver cartridge and centrifugal ultracentrifugation, Mg concentrations were determined by ICP-MS. 25 Mg enrichments in blood cells from Mg-deficient rats were greater than those from controls. In humans, 25 , SeMet, SeEt and TmSe) by gel-permeation chromatography and ICP-MS was developed.…”