Specific diagnosis of lymph node micrometastasis in breast cancer by targeting activatable near-infrared fluorescence imaging

Zhao, Dan; Xu, Menghong; Yang, Shiyuan; Ma, Huide; Li, Huiwen; Wu, Rong; He, Yu; Wang, Shumin; Liang, Xiaolong

doi:10.1016/j.biomaterials.2022.121388

Cited by 30 publications

(14 citation statements)

References 36 publications

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“…In the diagnosis of lymph node micrometastasis in breast cancer, a frequently used method (i.e., ultrasonic imaging) was confused by poor resolution and contrast agent spillover. To solve this problem, researchers [ 91 ] conjugated mannose with IR780, a near-infrared fluorescence molecule, through cystamine. Then, the coupling compound self-assembled into an M2 macrophage-targeted fluorescent probe (MR780 NPs).…”

Section: Principlesof Designing Tams Imaging Nanoprobesmentioning

confidence: 99%

“… ( a ) Schematic diagram for the fabrication and function of MR780 NPs: 4-aminophenyl α-d-pyranoside and IR780 was connected via cystamine to obtain MR780 conjugate, and then self-assembled with DSPE-PEG2000 to prepare MR780 NPs. ( b ) Fluorescence imaging of lymph nodes in normal mice and plantar 4T1 tumor-bearing mice every 5 days [ 91 ]. Copyright 2022, Elsevier.…”

Section: Figurementioning

confidence: 99%

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Targeting Tumor-Associated Macrophages for Imaging

2022

Pharmaceutics

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As an important component of the tumor immune microenvironment (TIME), tumor-associated macrophages (TAMs) occupy a significant niche in tumor margin aggregation and respond to changes in the TIME. Thus, targeting TAMs is important for tumor monitoring, surgical guidance and efficacy evaluation. Continuously developing nanoprobes and imaging agents paves the way toward targeting TAMs for precise imaging and diagnosis. This review summarizes the commonly used nanomaterials for TAM targeting imaging probes, including metal-based nanoprobes (iron, manganese, gold, silver), fluorine-19-based nanoprobes, radiolabeled agents, near-infrared fluorescence dyes and ultrasonic nanobubbles. Additionally, the prospects and challenges of designing nanomaterials for imaging and diagnosis (targeting efficiency, pharmacokinetics, and surgery guidance) are described in this review. Notwithstanding, TAM-targeting nanoplatforms provide great potential for imaging, diagnosis and therapy with a greater possibility of clinical transformation.

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Section: Principlesof Designing Tams Imaging Nanoprobesmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

Targeting Tumor-Associated Macrophages for Imaging

2022

Pharmaceutics

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“…Since early detection of metastatic cancer has been absolutely emphasized for treatment and prognosis, innovative diagnostic methods are being studied [9][10][11][12][13][14], such as detecting cancerspecific markers through liquid biopsy besides the common diagnosis, including imaging examinations and histopathologic examinations. A liquid biopsy is a biological fluid sample that can be obtained from the body, such as blood, saliva, urine, or spinal fluid [15].…”

Section: Introductionmentioning

confidence: 99%

NCK-associated protein 1 regulates metastasis and is a novel prognostic marker for colorectal cancer

et al. 2023

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Metastatic colorectal cancer (CRC) remains a substantial problem for mortality and requires screening and early detection efforts to increase survival. Epithelial-mesenchymal transition (EMT) and circulation of tumor cells in the blood play important roles in metastasis. To identify a novel target for metastasis of CRC, we conducted a gene microarray analysis using extracted RNA from the blood of preclinical models. We found that NCK-associated protein 1 (NCKAP1) was significantly increased in the blood RNA of patient-derived xenograft (PDX) models of colon cancer. In the NCKAP1 gene knockdown-induced human colon cancer cell lines HCT116 and HT29, there was a reduced wound healing area and significant inhibition of migration and invasion. As the result of marker screening for cytoskeleton and cellular interactions, CRC treated with siRNA of NCKAP1 exhibited significant induction of CDH1 and phalloidin expression, which indicates enhanced adherent cell junctions and cytoskeleton. In HCT116 cells with a mesenchymal state induced by TGFβ1, metastasis was inhibited by NCKAP1 gene knockdown through the inhibition of migration, and there was increased CTNNB1 expression and decreased FN expression. We established metastasis models for colon cancer to liver transition by intrasplenic injection shRNA of NCKAP1-transfected HCT116 cells or by implanting tumor tissue generated with the cells on cecal pouch. In metastasis xenograft models, tumor growth and liver metastasis were markedly reduced. Taken together, these data demonstrate that NCKAP1 is a novel gene regulating EMT that can contribute to developing a diagnostic marker for the progression of metastasis and new therapeutics for metastatic CRC treatment.

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“…Besides, the preservation of lymph nodes is essential, as lymph node-resident immune cells play important roles in cancer immunotherapy [ 11 , 12 ]. Although various imaging probes have been studied for use in SLN imaging and mapping [ 4 , 11 , 12 , 13 , 14 , 15 , 16 , 17 ], there are only a few reports on systems that allow the visualization of tumor cells in lymph nodes [ 18 , 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a Dual-Color Fluorescent Dendrimer for Diagnosis of Cancer Metastasis in Lymph Nodes

Kojima

Nagai

2022

Polymers

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Detection of cancer metastasis spread in lymph nodes is important in cancer diagnosis. In this study, a fluorescence imaging probe was designed for the detection of both lymph node and tumor cells using always-ON and activatable fluorescence probes with different colors. Rhodamine B (Rho), a matrix metalloproteinase−2 (MMP−2)-responsive green fluorescence probe, and a tumor-homing peptide were conjugated to a carboxy-terminal dendrimer that readily accumulates in lymph nodes. The activatable green fluorescence signal increased in the presence of MMP−2, which is secreted by tumor cells. Both the always-ON Rho signal and the activatable green fluorescence signal were observed from tumor cells, but only the weak always-ON Rho signal was from immune cells. Thus, this type of dendrimer may be useful for non-invasive imaging to diagnose cancer metastasis in lymph nodes.

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Specific diagnosis of lymph node micrometastasis in breast cancer by targeting activatable near-infrared fluorescence imaging

Cited by 30 publications

References 36 publications

Targeting Tumor-Associated Macrophages for Imaging

Targeting Tumor-Associated Macrophages for Imaging

NCK-associated protein 1 regulates metastasis and is a novel prognostic marker for colorectal cancer

Synthesis of a Dual-Color Fluorescent Dendrimer for Diagnosis of Cancer Metastasis in Lymph Nodes

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