Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti

Carlisle, Francesca A.; Steel, Karen P.; Lewis, Morag A.

doi:10.1016/j.gep.2012.03.001

Cited by 12 publications

(11 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with previous observations (35,36), robust and extensive labeling for acetyl-tubulin and Odf2 were detected in control root cell processes. In contrast, the mutant cochlea displayed the expression of these two molecules largely restricted to the immature outer sulcus epithelium lining the scala media (Fig.…”

Section: Zbtb20 Deletion Causes Severe Defects In Root Cell Different...supporting

confidence: 92%

ZBTB20 is Essential for Cochlear Maturation and Hearing in Mice

Xie

Bai

et al. 2022

Preprint

View full text Add to dashboard Cite

The mammalian cochlear epithelium undergoes substantial remodeling and maturation before the onset of hearing. However, very little is known about the transcriptional network governing cochlear late-stage maturation and particularly the differentiation of its lateral non-sensory region. Here we establish ZBTB20 as an essential transcription factor required for cochlear terminal differentiation and maturation and hearing. ZBTB20 is abundantly expressed in the developing and mature cochlear non-sensory epithelial cells, with transient expression in immature hair cells and spiral ganglion neurons. Otocyst-specific deletion of Zbtb20 causes profound deafness with reduced endolymph potential in mice. The subtypes of cochlear epithelial cells are normally generated but their postnatal development is arrested in the absence of ZBTB20, as manifested by an immature appearance of the organ of Corti, malformation of tectorial membrane, a flattened spiral prominence, and a lack of identifiable Boettcher cells. Furthermore, these defects are related with a failure in the terminal differentiation of the non-sensory epithelium covering the outer border Claudius cells, outer sulcus root cells and spiral prominence epithelial cells. Transcriptome analysis shows ZBTB20 regulates genes coding for tectorial membrane proteins in the greater epithelial ridge, and those preferentially expressed in root cells and spiral prominence epithelium. Our results point to ZBTB20 as an essential regulator for postnatal cochlear maturation and particularly for the terminal differentiation of cochlear lateral non-sensory domain.

show abstract

Section: Zbtb20 Deletion Causes Severe Defects In Root Cell Different...supporting

confidence: 92%

ZBTB20 is Essential for Cochlear Maturation and Hearing in Mice

Xie

Bai

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…These data indicate that KCNA10 expression develops in the inner and outer hair cells in opposing directions with respect to the base and apex of the cochlea. No detectable beta-galactosidase signal corresponding to KCNA10 expression was observed in other cochlear compartments, including those reported by Carlisle et al (2012) having positive anti-KCNA10 antibody immunoreactivity, such as the stria vascularis, Reissner’s membrane, Hensen’s cells, Deiters’ cells, root cells, Kolliker’s organ, interdental cells, marginal cells, and spiral prominence.…”

Section: Resultsmentioning

confidence: 72%

“…Carlisle et al (2012) used an antibody to an epitope corresponding to amino acids 154 to 203 in human and mouse KCNA10 and observed labelling in many more cell types (Reissner’s membrane, neurons, stria vascularis, etc.) than indicated by our beta-galactosidase expression.…”

Section: Discussionmentioning

confidence: 99%

“…KCNA10 has been localized to rat glomerular endothelium and renal proximal tubule by immunohistochemistry, where it is hypothesized to play a role in stabilizing cell membrane voltage (Yao et al, 2002). Recently, KCNA10 was localized to the mouse organ of Corti and vestibular end-organs by immunohistochemistry (Carlisle et al 2012). Inspection of Kcna10 signature sequences in more than 90 tissue-specific Massively Parallel Signature Sequencing (MPSS) cDNA libraries (Peters et al, 2007) indicates selective, almost exclusive, expression of this gene in the inner ear.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A null mutation of mouse Kcna10 causes significant vestibular and mild hearing dysfunction

et al. 2013

View full text Add to dashboard Cite

KCNA10 is a voltage gated potassium channel that is expressed in the inner ear. The localization and function of KCNA10 was studied in a mutant mouse, B6-Kcna10TM45, in which the single protein coding exon of Kcna10 was replaced with a beta-galactosidase reporter cassette. Under the regulatory control of the endogenous Kcna10 promoter and enhancers, beta-galactosidase was expressed in hair cells of the vestibular organs and the organ of Corti. KCNA10 expression develops in opposite tonotopic gradients in the inner and outer hair cells. Kcna10TM45 homozygotes display only a mild elevation in pure tone hearing thresholds as measured by auditory brainstem response (ABR), while heterozygotes are normal. However, Kcna10TM45 homozygotes have absent vestibular evoked potentials (VsEPs) or elevated VsEP thresholds with prolonged peak latencies, indicating significant vestibular dysfunction despite the lack of any overt imbalance behaviors. Our results suggest that Kcna10 is expressed primarily in hair cells of the inner ear, with little evidence of expression in other organs. The Kcna10TM45 targeted allele may be a model of human nonsyndromic vestibulopathy.

show abstract

“…An interesting question is why a protein like Odf2, which is not exclusively expressed in RGPs but also in many other different cell types (Carlisle et al 2012, Ibi et al 2011, Kunimoto et al 2012, Schweizer & Hoyer-Fender 2009 is regulated by such a cell type specific TF as Pax6. One possibility may be that Odf2 expression in differentiated cells is regulated by a transcription factor, which is missing in stem cells.…”

Section: Iii3 Put the Things Togethermentioning

confidence: 99%

Transcription factor Pax6 controls structure and function of the centrosome in cortical progenitors

Tylkowski¹

View full text Add to dashboard Cite

show abstract

Specific expression of Kcna10, Pxn and Odf2 in the organ of Corti

Cited by 12 publications

References 52 publications

ZBTB20 is Essential for Cochlear Maturation and Hearing in Mice

ZBTB20 is Essential for Cochlear Maturation and Hearing in Mice

A null mutation of mouse Kcna10 causes significant vestibular and mild hearing dysfunction

Transcription factor Pax6 controls structure and function of the centrosome in cortical progenitors

Contact Info

Product

Resources

About