Specific expression of the chicken δ-crystallin gene in the lens and the pyramidal neurons of the piriform cortex in transgenic mice

Kondoh, Hisato; Kato, Kazuto; Takahashi, Yoshiko; Fujisawa, Hajime; Yokoyama, Minesuke; Kimura, Shiari; Katsuki, Motoya; Saito, Muneo; Nomura, Tatsuji; Hiramoto, Yukio; Okada, Tamotsu

doi:10.1016/0012-1606(87)90116-3

Cited by 72 publications

(29 citation statements)

References 26 publications

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“…Since we confirmed that the 81-crystallin enhancer exerted its lens-specific effect also in mouse Cold Spring Harbor Laboratory Press on May 10, 2018 -Published by genesdev.cshlp.org Downloaded from cells (K. Goto, S. Hayashi, and H. Kondoh, unpubl. ), this enhancer effect would account for previous observations that the chicken 81-crystallin gene was correctly regulated in cultured mouse cells (Kondoh et al 1983;Hayashi et al 1985;) and in transgenic mice (Kondoh et al 1987).…”

Section: Discussionsupporting

confidence: 67%

“…As we have previously reported (Kondoh et al 1987), parts of subsegments B1 and B2 have a certain degree of homology to a middle-repetitive sequence found in an intron of the rat growth hormone gene {Barta et al 1981; Page et al 1981). However, ACAAT is not found in the repetitive sequence.…”

mentioning

confidence: 75%

“…Although the 81-crystallin promoter is nonspecific, the 81-crystallin gene is regulated in a lens-specific manner in cultured mouse cells (Kondoh et al 1983;Hayashi et al 1985;) and in transgenic mice (Kondoh et al 1987). Therefore, assuming that transcriptional regulation is the basis for lens-specificity, we suspected the presence of a control element that is separate from the promoter and functions in a way analogous to viral enhancers (Serfling et al 1985).…”

Section: Third Intron Of 61-crystallin Gene Bears a Lens-specific Enhmentioning

confidence: 99%

“…Introduction of the molecular-cloned 81-crystallin gene into mouse cells in primary culture (Kondoh et al 1983) and into transgenic mice (Kondoh et al 1987) resulted in proper regulation of the gene with respect to tissue specificity, indicating that DNA elements essential for lens-specific gene regulation are in fact located within the introduced DNA sequence. We have also shown that the region upstream of position -100 is not necessary for lens-specific expression (Hayashi et al 1985).…”

mentioning

confidence: 99%

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Lens-specific enhancer in the third intron regulates expression of the chicken delta 1-crystallin gene.

Hayashi

Goto²,

Okada³

et al. 1987

Genes Dev.

116

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We have previously shown that the tissue specificity determinant of the chicken ~l-crystallin gene lies 3' of position -100 (Hayashi et al. 1985). Since the promoter of the gene (81-crystallin promoter) did not show any tissue specificity, we examined various segments of the ~l-crystallin gene for a tissue-specific enhancer activity by placing each segment downstream of a heterologous transcriptional unit coding for chloramphenicol acetyltransferase (CAT) and by transfecting chicken tissues in primary culture. We found that a segment spanning the third intron bears a strong lens-specific enhancer activity. This "~l-crystallin enhancer" activates transcription from the 81-crystallin promoter 20-to 40-fold in lens cells and to various degrees with other promoters. Deletion analysis of the enhancer region indicated that it covered nearly 1 kb but did not indicate clear-cut boundaries. For its enhancer effect the core region of 120 bp and associations with certain adjoining regions were required. Removal of the enhancer from the gene totally abolished ~l-crystallin expression, and reinsertion of the enhancer in either upstream, internal, or downstream positions restored expression. We conclude that the ~l-crystallin enhancer is an essential and major determinant for lens-specificity of 81-crystallin expression.

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Section: Discussionsupporting

confidence: 67%

mentioning

confidence: 75%

Section: Third Intron Of 61-crystallin Gene Bears a Lens-specific Enhmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Lens-specific enhancer in the third intron regulates expression of the chicken delta 1-crystallin gene.

Hayashi

Goto²,

Okada³

et al. 1987

Genes Dev.

116

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show abstract

“…Transfection (Hayashi et al, 1987;Thomas et al, 1990;Goto et al, 1990) and transgenic mouse (Kondoh et al, 1987) experiments have established the importance of transcriptional events for controlling 6-crystallin gene expression in lens and non-lens tissues. For example, transgenic mice expressed a fusion gene composed of a 62-crystallin promoter fragmenthacterial chloramphenicol acetyltransferase reporter genel62-crystallin enhancer (from intron 3) in the lens, neuroretina and brain (Purkinje cells of the cerebellum), reminescent of the expression pattern of the ASLl62-crystallin gene in the chicken embryo (Li et al, 1993a(Li et al, , 1993b; see cover immunofluorescence photomicrograph of this issue).…”

Section: Gene Sharing and Differences In Evolutionary Pathways Among mentioning

confidence: 99%

Puzzle of crystallin diversity in eye lenses

Piatigorsky

1993

Developmental Dynamics

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Crystallins have evolved by various mechanisms that are associated with high expression of their genes in the eye lens. The diversity and pattern of crystallins among different species indicate that independent events have occurred at the molecular level during the evolution of the lens in different invertebrates (jellyfish, squid, and octopus) and vertebrates. Although it is possible that different crystallins are needed to fulfill the specific needs of individual species, the unexpectedly large array of proteins that function as crystallins and their abundance in the lens raise the possibility that selective pressures optimizing the function of certain transcription factors in the lens contribute to the recruitment of crystallins. 0 1993 Wiley-Liss, Inc.

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Lens-preferred activity of chicken δ1- and δ2-crystallin enhancers in transgenic mice and evidence for retinoic acid-responsive regulation of the δ1-crystallin gene

Li¹,

Cvekl²,

Bassnett³

et al. 1997

Dev. Genet.

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Specific expression of the chicken δ-crystallin gene in the lens and the pyramidal neurons of the piriform cortex in transgenic mice

Cited by 72 publications

References 26 publications

Lens-specific enhancer in the third intron regulates expression of the chicken delta 1-crystallin gene.

Lens-specific enhancer in the third intron regulates expression of the chicken delta 1-crystallin gene.

Puzzle of crystallin diversity in eye lenses

Lens-preferred activity of chicken δ1- and δ2-crystallin enhancers in transgenic mice and evidence for retinoic acid-responsive regulation of the δ1-crystallin gene

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