Specific expression of ZmPRL, the maize homolog of MCM7, during early embryogenesis

Bastida, Miriam; Puigdomènech, Pere

doi:10.1016/s0168-9452(01)00537-4

Cited by 14 publications

(18 citation statements)

References 45 publications

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“…PRL functions in early embryogenesis (9) and cytokinesis during seed development (20), implicating MCM7 in rapid cell division. Consistent with the function of AtMCM7, a maize MCM7 homologue (ZmPRL) is preferentially expressed during early embryogenesis (21). Another member of this MCM gene family (MCM3) is also predominantly expressed in proliferating cells, similarly to MCM7, in both maize (8,22) and Arabidopsis (23).…”

Section: Introductionmentioning

confidence: 74%

Identification and characterization of a rice MCM2 homologue required for DNA replycation

Cho

Kim²,

Kim

et al. 2008

BMB Reports

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The pre-replication complex (pre-RC), including the core hexameric MCM2-7 complex, ensures that the eukaryotic genome is replicated only once per cell division cycle. In this study, we identified a rice minichromosome maintenance (MCM) homologue (OsMCM2) that functionally complemented fission yeast MCM2 (CDC19) mutants. We found OsMCM2 transcript expression in roots, leaves, and seeds, although expression levels differed slightly among the organs. Likewise, the OsMCM2 protein was ubiquitously expressed, but it was downregulated when nutritients were limiting, indicating that MCM2 expression (and therefore cell cycle progression) requires adequate nutrition.

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Section: Introductionmentioning

confidence: 74%

Identification and characterization of a rice MCM2 homologue required for DNA replycation

Cho

Kim²,

Kim

et al. 2008

BMB Reports

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“…Previous studies in Arabidopsis and maize showed that MCM3 (Sabelli et al, 1996;Stevens et al, 2002), MCM6 (Dresselhaus et al, 2006), and MCM7 (Springer et al, 1995(Springer et al, , 2000Bastida and Puigdomenech, 2002;Holding and Springer, 2002) are expressed in young tissues that contain actively replicating cells, but it has not been clear whether the entire complex is coordinately expressed. We showed that the relative mRNA abundance for each of the six MCM subunits was highly correlated with replication potential and that the entire complex was coordinately expressed across 11 tissue types representing various stages of Arabidopsis vegetative and floral development ( Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Consistent with a role in DNA replication, MCM genes from Arabidopsis (Springer et al, 1995(Springer et al, , 2000Holding and Springer, 2002;Stevens et al, 2002) and maize (Sabelli et al, 1996;Bastida and Puigdomenech, 2002;Dresselhaus et al, 2006) are preferentially expressed in young tissues that contain a high number of replicating cells.…”

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confidence: 91%

“…MCM subunits have been identified in diverse plant genomes, including Arabidopsis (Arabidopsis thaliana; Springer et al, 1995;Stevens et al, 2002;Masuda et al, 2004;Dresselhaus et al, 2006;Shultz et al, 2007), rice (Oryza sativa;Shultz et al, 2007), maize (Zea mays; Sabelli et al, 1996Sabelli et al, , 1999Bastida and Puigdomenech, 2002;Dresselhaus et al, 2006), and tobacco (Nicotiana tabacum; Dambrauskas et al, 2003). Consistent with a role in DNA replication, MCM genes from Arabidopsis (Springer et al, 1995(Springer et al, , 2000Holding and Springer, 2002;Stevens et al, 2002) and maize (Sabelli et al, 1996;Bastida and Puigdomenech, 2002;Dresselhaus et al, 2006) are preferentially expressed in young tissues that contain a high number of replicating cells.…”

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confidence: 99%

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Dynamic Localization of the DNA Replication Proteins MCM5 and MCM7 in Plants

et al. 2009

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Genome integrity in eukaryotes depends on licensing mechanisms that prevent loading of the minichromosome maintenance complex (MCM2-7) onto replicated DNA during S phase. Although the principle of licensing appears to be conserved across all eukaryotes, the mechanisms that control it vary, and it is not clear how licensing is regulated in plants. In this work, we demonstrate that subunits of the MCM2-7 complex are coordinately expressed during Arabidopsis (Arabidopsis thaliana) development and are abundant in proliferating and endocycling tissues, indicative of a role in DNA replication. We show that endogenous MCM5 and MCM7 proteins are localized in the nucleus during G1, S, and G2 phases of the cell cycle and are released into the cytoplasmic compartment during mitosis. We also show that MCM5 and MCM7 are topologically constrained on DNA and that the MCM complex is stable under high-salt conditions. Our results are consistent with a conserved replicative helicase function for the MCM complex in plants but not with the idea that plants resemble budding yeast by actively exporting the MCM complex from the nucleus to prevent unauthorized origin licensing and rereplication during S phase. Instead, our data show that, like other higher eukaryotes, the MCM complex in plants remains in the nucleus throughout most of the cell cycle and is only dispersed in mitotic cells.

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“…Genes expressed in the different regions are likely to be involved in the formation of different structures or organs. We have identified a gene, ZmPRL, which is more abundantly expressed in the embryo shoot region (Bastida et al, 2002). ZmPRL encodes a MCM7 protein required for DNA replication to ensure that only one round of replication takes place in each cell cycle.…”

Section: Gene Discovery In Maize Embryogenesismentioning

confidence: 99%

Genetic, molecular and cellular approaches to the analysis of maize embryo development

Josè-Estanyol

López-Ribera²,

Bastida³

et al. 2009

Int. J. Dev. Biol.

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The development of embryo structures in plants is essential for the formation of the adult plant organs. In cereals, this process has distinct features which have attracted attention from different points of view. Differential gene expression analyses have been used in order to identify genes useful as molecular markers of certain physiological, molecular or developmental processes. Several maize mutants affected in embryo development have been isolated, but only a fraction of them have been characterized at the molecular level. Molecular markers can be useful in the characterization of embryo defective mutants. Here, we describe the different techniques used in the identification of molecular marker genes for embryo development. We describe in more detail some groups of genes coding for cell wall proteins. We also describe the application of these molecular markers in the characterization of some embryo mutants.

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Specific expression of ZmPRL, the maize homolog of MCM7, during early embryogenesis

Cited by 14 publications

References 45 publications

Identification and characterization of a rice MCM2 homologue required for DNA replycation

Identification and characterization of a rice MCM2 homologue required for DNA replycation

Dynamic Localization of the DNA Replication Proteins MCM5 and MCM7 in Plants

Genetic, molecular and cellular approaches to the analysis of maize embryo development

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