Specific features of changes in levels of endogenous respiration substrates in Saccharomyces cerevisiae cells at low temperature

Abstract

The rate of endogenous respiration of Saccharomyces cerevisiae cells incubated at 0 degrees C under aerobic conditions in the absence of exogenous substrates decreased exponentially with a half-period of about 5 h when measured at 30 degrees C. This was associated with an indirectly shown decrease in the level of oxaloacetate in the mitochondria in situ. The initial concentration of oxaloacetate significantly decreased the activity of succinate dehydrogenase. The rate of cell respiration in the presence of acetate and other exogenous substrates producing acetyl-CoA in mitochondria also decreased, whereas the respiration rate on succinate increased. These changes were accompanied by an at least threefold increase in the L-malate concentration in the cells within 24 h. It is suggested that the increase in the L-malate level in the cells and the concurrent decrease in the oxaloacetate level in the mitochondria should be associated with a deceleration at 0 degrees C of the transport of endogenous respiration substrates from the cytosol into the mitochondria. This deceleration is likely to be caused by a high Arrhenius activation energy specific for transporters. The physiological significance of L-malate in regulation of the S. cerevisiae cell respiration is discussed.

Industrial Systems Biology of Saccharomyces cerevisiae Enables Novel Succinic Acid Cell Factory

“…With this strategy, glycine and serine biomass requirements are directly coupled to succinate formation via the glyoxylate cycle. Substantial succinate accumulation (defined as >0.1 g L − 1 ) in the culture broth is not observed in wild-type S. cerevisiae , and deletion of sdh3 has not resulted in appreciable succinate accumulation [17]; a conclusion also found by chemical inhibition of the succinate dehydrogenase complex with titration of malonate (Figure S1), a chemical inhibtor of this complex [18].…”

Aerobic production and utilization of lactate satisfy increased energy demands upon neuronal activation in hippocampal slices and provide neuroprotection against oxidative stress

“…A newly discovered lactate dehydrogenase inhibitor, malonate, a known competitive inhibitor of succinate dehydrogenase, has been shown to inhibit the conversion of lactate to pyruvate by Saad et al (2006). This succinate analog is transported into neurons, astrocytes, and mitochondria via the succinate transporter (Aliverdieva et al, 2006; Yodoya et al, 2006). We used malonate in a set of experiments to test the hypothesis that lactate is the end-product of aerobic glycolysis in cerebral tissue (Schurr and Payne, 2007).…”

Different respiration metabolism between mycorrhizal and non-mycorrhizal rice under low-temperature stress: a cry for help from the host

“…The other physiological role of AOX is to prevent the excess generation of reactive oxygen species, which can damage a wide range of macromolecules, leading to cell death (Vanlerberghe et al 2009;Dinakar et al 2010a;Wang et al 2011). Moreover, several studies have demonstrated the existence of a close relationship between the AOX pathway and pyruvate (Pastore et al 2001;Dinakar et al 2010b), which is a key metabolite and can ensure the functioning of the TCA cycle in mitochondria (Aliverdieva et al 2006). Increases in AOX activity correlates well with the increase in intracellular pyruvate levels, and decreased pyruvate levels also lead to decreased AOX protein levels, signifying the importance of pyruvate in activating AOX (Oliver et al 2008;Dinakar et al 2010b).…”

“…2010 b ), which is a key metabolite and can ensure the functioning of the TCA cycle in mitochondria (Aliverdieva et al . 2006). Increases in AOX activity correlates well with the increase in intracellular pyruvate levels, and decreased pyruvate levels also lead to decreased AOX protein levels, signifying the importance of pyruvate in activating AOX (Oliver et al .…”