Specific features of the erythroid hemopoietic stem in CBA/CaLac mice with neuroses demonstrating good and poor learning capacities

Abstract

We studied changes in the erythroid hemopoietic stem in CBA/CaLac mice with experimental neuroses demonstrating good and poor learning capacities (conflict situation and paradoxical sleep deprivation followed by training in a 3-arm T-maze). The animals with different learning capacities exhibited pronounced hyperplasia of the erythroid hemopoietic stem in response to neurosis. Activation of the erythron in good learners was related to acceleration of division and maturation of erythroid precursors and enhanced formation of cell complexes containing central macrophage. In poor learners hyperplasia of the erythroid hemopoietic stem under conditions of conflict situation manifested in activation of proliferation and differentiation (against the background of decreased count of erythroid and mixed complexes in the bone marrow), while after paradoxical sleep deprivation followed by T-maze training this hyperplasia was associated with increased formation of additional hemopoietic islets (against the background of desynchronization of division and maturation of erythroid precursor cells).

Neurosis-Associated Changes in the Granulocytic Hemopoietic Stem in Mice with Different Learning Capacity

“…Our experiments demonstrated that good and poor learners are characterized by high and low locomotor activity in the open field, respectively. These data suggest that dif- (2,4) subjected to conflict situation (1,2) or paradoxical sleep deprivation and training in a 3-arm T-maze (3,4). Ordinate: number of hemopoietic islets in the bone marrow, ×10 3 .…”

“…These changes result from increased formation of granulocytic and erythrogranulocytic hemopoietic islets. Initial suppression of the bone marrow granulocytopoiesis in poor learners under conditions of CS is as- (2,4) subjected to conflict situation (1,2) or paradoxical sleep deprivation and training in a 3-arm T-maze (3,4). Ordinate: number of granulocyte-macrophage precursors (content of colonies and clusters, ×10 5 cells, a, b, f, g); ratio of precursor cells in S-phase of the mitotic cycle (%, c, d, h, i); index of maturation (clusters/colonies ratio in a well, e, j).…”

“…The division of animals by their open-field behavior is mainly based on nonassociative learning and, to a lesser extent, on individual characteristics of their higher nervous activity. Our previous studies revealed different changes in the erythroid hemopoietic stem during experimental neuroses in mice exhibiting good and poor learning in a 3-arm T-maze [4]. The observed intergroup differences were determined by functional activity of the local system of erythropoiesis regulation (hemopoiesis-inducing microenvironment).…”

Monoaminergic Regulation of the Pool of Erythropoietic Stem Cells in Active and Passive Mouse in Experimental Neuroses

“…The pool of erythroid precursor cells in CBA/CaLac mice of different behavioral types demonstrates different reaction to experimental neurotic sates [8]: the intensity of proliferation and differentiation of erythropoietic precursors increase in active animals, while in passive mice we observe dysregulation of these processes.…”

“…Ten days before neurosis modeling the animals were divided into passive and active by the type of their behavior in a 3-component T-maze [8]. The animals were sacrificed by cervical dislocation under ether narcosis on days 1, 2, 4, and 5 after neurosis modeling and the number of erythrokaryocytes in the bone marrow was determined [5].…”