Influence of the oxygen stoichiometry on the phase diagram of clean YBa 2 Cu 3 O 7Ϫ␦ single crystals has been studied in the region of optimal doping (0.05Ͻ␦Ͻ0.09). Temperature and angular dependencies of magnetoresistance were analyzed for fields of up to 23 T. At low fields a sharp resistivity kink, which is a signature of the first order melting transition, was observed in all the samples studied. This kink, however, disappears when B exceeds the multicritical field B mc . It was found that B mc strongly increases with decreasing ␦. We have demonstrated that variations in the oxygen stoichiometry in the range of 0.05Ͻ␦Ͻ0.09 do not have a significant effect on intrinsic parameters ͑such as anisotropy, coherence length, and penetration depth͒ but lead to a strong increase in entanglement of the vortex lines. We relate the dependence of B mc on ␦ with entanglement of the vortex lines, which is promoted by their interaction with oxygen vacancies.