Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses

Xiong, Hairong; Li, Q.; Chen, Wei; Liu, D.-Y.; Ling, Jiaxin; Liu, J.; Liu, Y.-J.; Zhang, Y.; Yang, Zunhua

doi:10.1007/s10096-010-1134-5

Cited by 7 publications

(6 citation statements)

References 28 publications

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“…Given the peak periods of HFRS identified in our seasonal analysis, we infer that HFRS was primarily caused by HTNV in the 1980's and 1990's. This is consistent with previous findings indicating that the majority of patients with HFRS are infected with HTNV [32]. However, during the last decade, the HFRS has begun to occur more frequently in the spring and summer, accounting for about 50% of all cases.…”

Section: Discussionsupporting

confidence: 93%

The Epidemic Characteristics and Changing Trend of Hemorrhagic Fever with Renal Syndrome in Hubei Province, China

Yihui

Liu

et al. 2014

PLoS ONE

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BackgroundHemorrhagic fever with renal syndrome (HFRS) is caused by different hantaviruses within the Bunyaviridae family. HFRS is a fulminant, infectious disease that occurs worldwide and is endemic in all 31 provinces of China. Since the first HFRS case in Hubei Province was reported in 1957, the disease has spread across the province and Hubei has become one of the seriously affected areas in China with the greatest number of reported HFRS cases in the 1980's. However, the epidemic characteristics of HFRS in Hubei are still not entirely clear and long-term, systematic investigations of this epidemic area have been very limited.MethodsThe spatiotemporal distribution of HFRS was investigated using data spanning the years 1980 to 2009. The annual HFRS incidence, fatality rate and seasonal incidence between 1980 and 2009 were calculated and plotted. GIS-based spatial analyses were conducted to detect the spatial distribution and seasonal pattern of HFRS. A spatial statistical analysis, using Kulldorff's spatial scan statistic, was performed to identify clustering of HFRS.ResultsA total of 104,467 HFRS cases were reported in Hubei Province between 1980 and 2009. Incidence of and mortality due to HFRS declined after the outbreak in 1980s and HFRS cases have been sporadic in recent years. The locations and scale of disease clusters have changed during the three decades. The seasonal epidemic pattern of HFRS was characterized by the shift from the unimodal type (autumn/winter peak) to the bimodal type.ConclusionsSocioeconomic development has great influence on the transmission of hantaviruses to humans and new epidemic characteristics have emerged in Hubei Province. It is necessary to reinforce preventative measures against HFRS according to the newly-presented seasonal variation and to intensify these efforts especially in the urban areas of Hubei Province.

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Section: Discussionsupporting

confidence: 93%

The Epidemic Characteristics and Changing Trend of Hemorrhagic Fever with Renal Syndrome in Hubei Province, China

Yihui

Liu

et al. 2014

PLoS ONE

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“…Numerous studies have identified the N protein as highly conserved among orthohantaviruses, making it a valuable target for diagnosing and developing vaccine candidates [11]. Studies support this assumption; the reactivity of recombinant PUUV N proteins was demonstrated in HFRS convalescent samples [13]. It was reported that anti-N protein IgM was detected in the serum at the acute phase of infection, and IgG was detected in convalescent sera at greater levels.…”

Section: Introductionmentioning

confidence: 95%

Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus

Sehgal,

Sharma,

Kaushal

et al. 2024

Viruses

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Puumala orthohantavirus (PUUV) is an emerging zoonotic virus endemic to Europe and Russia that causes nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome (HFRS). There are limited options for treatment and diagnosis of orthohantavirus infection, making the search for potential immunogenic candidates crucial. In the present work, various bioinformatics tools were employed to design conserved immunogenic peptides containing multiple epitopes of PUUV nucleocapsid protein. Eleven conserved peptides (90% conservancy) of the PUUV nucleocapsid protein were identified. Three conserved peptides containing multiple T and B cell epitopes were selected using a consensus epitope prediction algorithm. Molecular docking using the HPEP dock server demonstrated strong binding interactions between the epitopes and HLA molecules (ten alleles for each class I and II HLA). Moreover, an analysis of population coverage using the IEDB database revealed that the identified peptides have over 90% average population coverage across six continents. Molecular docking and simulation analysis reveal a stable interaction with peptide constructs of chosen immunogenic peptides and Toll-like receptor-4. These computational analyses demonstrate selected peptides’ immunogenic potential, which needs to be validated in different experimental systems.

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“…Patients were recruited from 12 districts in Hubei Province, China and were unrelated. All patients were diagnosed during hospitalization from 1986 to 1995 according to standard clinical criteria (Xiong et al, 2011), which mainly include: age ≥ 14 years, febrile time ≤ 4 days, typical HFRS symptoms and signs (e.g., fever, proteinuria, hematuria), and potential exposure history to wild rodents or other HFRS patients. Patient sera were subsequently confirmed to be positive by indirect IgM/IgG enzyme-linked immunosorbent assays (ELISAs) performed in our laboratory, using mixed antigens of HTNV and SEOV.…”

Section: Study Participantsmentioning

confidence: 99%

Lack of association between integrin αvβ3 gene polymorphisms and hemorrhagic fever with renal syndrome in Han Chinese from Hubei, China

et al. 2017

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Hantaviruses belong to the family Bunyaviridae and cause hemorrhagic fever with renal syndrome (HFRS) in humans. β integrins, including αβ and αβ integrins, act as receptors on endothelial cells and play key roles in cellular entry during the pathogenesis of hantaviruses. Previous study demonstrated that the polymorphisms of integrin αβ are associated with susceptibility to hantavirus infection and the disease severity of HFRS in Shaanxi Province of China, rather than in Finland. However, the polymorphisms of integrin αβ in patients with HFRS was incompletely understood. Here, we aimed to investigate the associations between polymorphisms in human integrin αβ and HFRS in Han Chinese individuals. Ninety patients with HFRS and 101 healthy controls were enrolled in this study. Analysis of five single nucleotide polymorphism (SNP) sites (rs3768777 and rs3738919 on ITGAV; rs13306487, rs5921, and rs5918 on ITGB3) was performed by TaqMan SNP genotyping assays and bi-directional PCR allele-specific amplification method. No significant differences were observed between the HFRS group and controls regarding the genotype and allele frequency distributions of any of the five SNP sites, and no associations were found between ITGAV polymorphisms/genotypes and disease severity. In conclusion, our results implied that these five SNPs in the integrin αβ gene were not associated with HFRS susceptibility or severity in Han Chinese individuals in Hubei Province.

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Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses

Cited by 7 publications

References 28 publications

The Epidemic Characteristics and Changing Trend of Hemorrhagic Fever with Renal Syndrome in Hubei Province, China

The Epidemic Characteristics and Changing Trend of Hemorrhagic Fever with Renal Syndrome in Hubei Province, China

Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of Puumala orthohantavirus

Lack of association between integrin αvβ3 gene polymorphisms and hemorrhagic fever with renal syndrome in Han Chinese from Hubei, China

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